鸭疫里默氏杆菌血清2型的分离鉴定及商品化疫苗免疫保护效果研究

doi:10.16431/j.cnki.1671-7236.2025.09.025

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the biological characteristics of Riemerella anatipestifer (RA),which was isolated from diseased ducks,and evaluate the protective effect of 3 commercially available vaccines for infectious serositis in ducks against the newly isolated strain through animal challenge tests,in order to provide a reference for the prevention and control of infectious serositis in ducks. 【Method】 The liver and brain tissues of diseased ducklings were collected to isolate pathogenic bacteria.The isolated strains were identified by morphological observation,16S rRNA gene PCR identification and sequencing,phylogenetic tree construction,and serotype typing.The growth curves of the isolated strains were determined by spectrophotometry and plate counting.The drug sensitivity was analyzed by disk diffusion method.The median lethal dose (LD₅₀) and tissue bacterial load were determined through animal experiments.Finally,the immune protective effects of 3 commercial inactivated vaccines on the isolated strains were evaluated through challenge tests. 【Result】 One strain of RA was isolated in this experiment,and it was serotype 2.The results of the drug sensitivity test showed that the isolate was resistant to 10 drugs such as ampicillin,ceftazidime and kanamycin,and was sensitive to amoxicillin,cefotaxime,florfenicol and streptomycin.The determination results of the growth curve showed that after 6-10 h of culture,the isolate entered the logarithmic growth phase,and the viable bacteria count could reach 10⁹ CFU/mL after 8 h of culture.The LD₅₀ for ducklings was 7.65×10^4.7 CFU,and the bacterial load in heart of ducklings was the highest after infection.2 weeks after immunizing 7-day-old duckings with 3 commercial inactivated vaccines,the isolate was used for challenge.The results showed that the multivalent RA inactivated vaccines of types 1+2+7 had the best protective effect,with a protection rate of up to 70%,while the protection rates of the other two vaccines were less than 50%. 【Conclusion】 In this study,one strain of multi-drug resistant and highly pathogenic RA serotype 2 was isolated，the immune protective effect of commercially available RA vaccines on the isolated was limited.This result provided a theoretical basis for the current prevention and control of infectious serositis in ducks.

Key words: Riemerella anatipestifer; serotype 2; biological characteristics; commercial vaccine

CLC Number:

S852.61

ZHANG Rongrong, WANG Zui, GUO Yunqing, LI Guohong, CHENG Yiluo, LU Qin, LUO Cong, ZHANG Tengfei, ZHANG Wenting, HU Qiao, LUO Qinping. Isolation and Identification of Riemerella anatipestifer Serotype 2 and the Evalution of Protective Efficacy of Commercial Vaccine[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4273-4283.

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Isolation and Identification of Riemerella anatipestifer Serotype 2 and the Evalution of Protective Efficacy of Commercial Vaccine

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