宏基因组分箱技术及其在猪肠道微生物组研究中的应用

doi:10.16431/j.cnki.1671-7236.2025.10.017

Abstract

Abstract: With the rapid development of metagenomics,binning technology has gradually become an indispensable tool for quantifying microbial communities.This technology clusters sequences from the same microorganism based on shared features using clustering algorithms,thereby separating the genomes of different microorganisms in high-throughput sequencing data,enabing single-strain genome reconstruction and species-specific association analysis.Due to its ability to provide high-resolution species annotation at the strain level,binning technology is gradually replacing traditional assemble-free metagenomic analysis methods.Currently,binning technology has been increasingly applied in veterinary and animal science,particularly in studies of the composition and dynamics of gut microbiota.As pigs are one of the most economically important livestock animals,and their gut microbiota significantly influences nutrition,health,disease resistance,and immune capacity,the authors introduce recent advances in the application of metagenomic binning technology in pig gut microbiome research,including its contributions to constructing reference catalogs for the pig gut microbiome and capturing microbial community shifts driven by breed,growth stage,diet,and environmental factors.These advancements not only enhance our understanding of the composition and function of the pig gut microbiota but also provide valuable technical references and theoretical support for future microbiome research in the livestock industry.

Key words: binning; metagenomic; pig; gut microbiota

CLC Number:

S828.9

ZHAO Jin, HE Zhaoyuan, MENG Shuling, LUO Guangwen, CHEN Hailan, JIA Gengjie. Study Progress on the Application of Metagenomic Binning Technology in the Pig Gut Microbiome[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4717-4724.

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Study Progress on the Application of Metagenomic Binning Technology in the Pig Gut Microbiome

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