Abstract: Applying reverse transcription LAMP (RT-LAMP), according to the N protein conserved sequence of PRRSV ORF5 genes from six regions, two sets of primers were designed. By optimizing reaction system and conditions, the RT-LAMP method for detecting PRRSV was developed after sensitivity and specificity tests. The research results indicate that PRRSV nucleic acids amplification can be completed within 1 h in the isothermy condition of 63 ℃ only using a common constant temperature water-bath, test results can be judged with naked eyes after adding nucleic acids dye SYBR GreenⅠ. It provided a kind of cheap, simple, speedy, high sensitivity and specificity PRRSV detecting method for work field and grass-roots units.

Key words: porcine reproductive and respiratory syndrome virus; RT-LAMP; detection method; research