抗氧化剂对反刍动物精液冷冻效果的影响

doi:10.16431/j.cnki.1671-7236.2025.07.025

Abstract

Abstract: Artificial insemination technology plays a significant role in livestock genetic improvement and reproductive management,while semen cryopreservation facilitates the full utilization of superior sires’ reproductive potential and reduces production costs in the breeding industry.With the popularization of artificial insemination technology,the requirements for the quality and time of cryopreservation of ruminant semen have been also increasing.However,sperms in semen are susceptible to ice crystal damage and oxidative stress damage during the freezing-thawing process,which affects sperm motility and fertilization ability.As an important substance to reduce oxidative damage and improve semen quality,exogenous antioxidants have attracted extensive attention in the cryopreservation of animal semen.At present,numerous studies have demonstrated the beneficial effects of exogenous antioxidants in ruminant semen cryopreservation,highlighting their substantial potential for improving frozen semen quality.To this end,this article systematically reviews the effects of ultra-low temperature on oxidative damage in sperm and how antioxidants protect sperm from oxidative stress by scavenging reactive oxygen species (ROS).It comprehensively discusses the application of both enzymatic and non-enzymatic antioxidants in ruminant semen cryopreservation,including specific antioxidant types,optimal concentrations,and their precise effects on sperm motility parameters.In addition,the potential application value of novel antioxidants (e.g.,kaempferol,rutin,astaxanthin) in the freezing of semen from ruminants are also explored.However,the optimal concentration,application mode and mechanism of action of antioxidants still require further investigation and refinement.By systematically analyzing the efficacy of various antioxidant types and concentrations in ruminant semen cryopreservation,this review aims to provide theoretical foundations and practical references for advancing and optimizing ruminant frozen semen technologies.

Key words: antioxidant; oxidative stress; sperm; semen freezing; ruminant

CLC Number:

S826

WANG Ru, WANG Jiahao, OU Jingyu, TANG Wenhui, CHENG Xiao, WANG Qiangjun, CHEN Jiahong, ZHANG Zijun, REN Chunhuan. Effect of Antioxidants on Freezing Effect of Semen in Ruminants[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3242-3255.

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Effect of Antioxidants on Freezing Effect of Semen in Ruminants

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