植物乳杆菌发酵培养物对单增李斯特菌感染肉鸡的治疗效果及机制

doi:10.16431/j.cnki.1671-7236.2020.08.037

Abstract

Abstract: The effects of Lactobacillus plantarum (L.plantarum) culture on the growth performance,oxidative reaction and MAPK signaling pathway of broilers infected with Listeria monocytogenes (L.monocytogenes) were investigated in this study for exploring the efficacy of L.plantarum culture against listeriosis and possible mechanism.A total of 480 one day old AA male broiler chicks were randomly allocated into 4 groups including 6 replicates per group of 20 chicks each replicate.Chicks in control and infection groups were offered a basal diet,whereas other two groups were supplied antibiotic enrofloxacin hydrochloride at 40 mg/kg or inactivated L.plantarum culture at 1.6 g/kg.Feeding trial lasted for 28 days.At 5 days old,chicks in infection,antibiotic and L.plantarum culture groups were orally administrated 1 mL L.monocytogenes (10⁵ CFU),whereas chicks in control group were offered the same liquid without the pathogen.The results showed that L.plantarum culure significantly improved ADWG,ADFI and final body weight of 7 and 28 days old broilers (P<0.05),significantly decreased feed/gain ratio (P<0.05),significantly decreased serum and duodenal mucosa concentrations of diamine oxidase,malondialdehyde and protein carbonyls (except for 14 d in duodenal mucosa) of 7 and 28 days old broilers (P<0.05),and significantly decreased mRNA levels of MAPK3,MAPK10 and DUSP6 (except for 7 d) genes of 7 and 28 days old broilers (P<0.05).The results suggested that dietary L.plantarum culture could inhibit L.monocytogenes infection,and improve antibacterial,antioxidative ability and anti-inflammatory ability through MAPK signaling.L.plantarum culture had a good therapeutic effect on L.monocytogenes infected broilers,and could be used as an alternative for antibiotics.

Key words: Lactobacillus plantarum culture; Listeria monocytogenes; growth performance; oxidative action; MAPK signaling

CLC Number:

S852.61

ZHENG Jiangping, LIU Ning, BAI Xuerui, SHANG Yueli. Effects and Mechanism of Lactobacillus plantarum Culture Against Listeria monocytogenes Infection in Broilers[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(8): 2666-2676.

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Effects and Mechanism of Lactobacillus plantarum Culture Against Listeria monocytogenes Infection in Broilers

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