奶牛微生物发酵饲料组方及其配套翻料工艺的研究

doi:10.16431/j.cnki.1671-7236.2016.10.013

Abstract

Abstract:

This experiment was conducted to study the effects of eight microbial fermentation feed formulas and the different turning processes on the yeast viable bacterial number and three kinds of nutritional active substance contents to determine the optimal formula and its turning process.Nine kinds of local agricultural products,such as wheat bran,rice bran,cottonseed meal and corn flour,were used as fermentation materials and eight different formulas were designed by mixture design of DPS.Saccharomyces cerevisiae BC,XR4 and Bacillus subtilis A15 were mixed by 2:2:1 and inoculated in the eight formulas to conduct solid state fermentation.The yeast viable bacterial number and the content of mannan,β-glucan and polypeptide were measured to screen the optimal formula.Basing on the optimal formula,the turning process experiment was conducted.There were three groups:Control group,experimental group 1 and 2.The control group was without turning process,while the feed in the experimental group 1 was turned once and the feed in the experimental group 2 was turned twice.The material temperature,yeast viable bacterial number and three kinds of nutritional active substance contents were measured to determine the optimal turning process.The results showed that:① Formula 8 was the optimized formula which consisted of 8.62% wheat bran,5.85% vinegar residue,20.89% rice bran,12.55% cottonseed meal,11.35% corn flour,7.31% corn bran,1.10% corn residue,25.36% sugar residue,5.35% corn germ meal,1.02% (NH₄)₂SO₄,0.50% KH₂PO₄ and 0.10% MgSO₄.② The best turning process of formula 8 was turning twice during the solid state fermentation.When the material temperature reached 35℃,the first turning was conducted and then when the temperature reached 42℃,the material would be turned again.After that the material continued fermenting until the end.Its optimal indexes were viable bacterial number was 4×10⁵ CFU/g,and the content of mannan,β-glucan and polypeptides were 41.28 mg/100 mg,87.06 mg/100 mg and 10.32 μg/100 mg,respectively.Its improved by 100.00%,3.90%,4.89% and 1.67% separately than the control group and improved by 33.33%,3.07%,3.31% and 0.88% comparing with the experimental group 1.

Key words: microbial fermentation feed; turning process; yeast viable bacterial number; nutritional active substance

CLC Number:

S816

GUO Peng, ZHANG Li-ming, ZHANG Zheng, ZHANG Qi-wei, LIU Da-cheng. Study on the Microbial Fermentation Feed Formula and Its Turning Process of Dairy Cows[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(10): 2598-2607.

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Study on the Microbial Fermentation Feed Formula and Its Turning Process of Dairy Cows

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