Abstract: This experiment was conducted to explore optimized conditions to make more Cashmere goat lymphocyte which had been treated in G2/M. Phytohaemagglutinin (PHA) and concanavalin A (ConA) reagents were used to stimulate the proliferation of lymphocytes,the colchicine reagent were used at specific times of lymphocytes cell cycle synchronization and the number of the cell in G/M was measured by flow cytometry to observe the optimal concentration and action time. The results showed that the PHA and ConA optimal concentration to stimulate the proliferation of lymphocytes were 60 and 5 μg/mL. In addition,lymphocytes with ConA stimulation culture 45 h,then colchicines was used to treat 5 h.The percentage of lymphocytes in G2/M accounted for 58.38%. The results suggested that the treatment effect of ConA worked better than PHA in proliferation of lymphocytes.

Key words: Cashmere goat; phytohaemagglutinin; concanavalin A; lymphocytes; colchicine; cell synchronization; flow cytometry