Abstract: The total RNA was abstracted from the concanavalin A(Con A) -activated spleen lymphocytes, a pair of specific primers was designed by Oligo software based on the sequence of chicken interferon-gamma submitted by digby in GenBank(accession No.U27465). A 500 bp DNA fragment was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and then ligated into T-easy vector for sequencing. The results showed that the fragment contained the complete open reading frame (ORF) of CHIFN-γ gene. In comparison with GenBank data, the homology of the nucleotide sequence was 100%.The sequence was inserted into pGEX-4T-1 and the recombined expressing vector was constructed, then transformed into Escherichia coli BL21, which were further induced by IPTG and cultured,and a fusion protein was obtained with about 43 ku of molecular weight in SDS-PAGE.This results showed that the cloned CHIFN-γ gene was expressed in prokaryotic cells.

Key words: interferon-γ; chicken; cloning; expression