China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (7): 58-60.

• 生物技术 • Previous Articles     Next Articles

Cloning, Sequencing and Analysis of IGF-1 Gene in Two Strains of Quial

FU Jing, LIU Zhao-yang, BAI Xiu-juan   

  1. (College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

Abstract: According to the sequence of chicken insulin-like growth factor (IGF-1) gene which submitted to GenBank, designing a pair of primers. The partial fragment of IGF-1 gene was cloned and sequenced on layer and meat quails. The length of the fragments were 806 bp and 804 bp. Now the two sequences had been sent to GenBank and registered comparison of the fragment sequence with that Gallus gallus IGF-1 gene was carried out through BLAST in GenBank, the result showed that the homologies of the nucleotide sequence was 93% with Gallus gallus. And two strains of quail had the homology of 99.38% in the nucleotide level.

Key words: quail; IGF-1 gene; cloning; sequencing

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