抗奶牛乳腺炎多价卵黄抗体的制备及含量测定

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (3): 134-136.

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Production and Content Detection of Multi-valent IgY against Cow Mastitis

ZHEN Yu-hong1，2, DIAO Yun-peng1, SHU Xiao-hong1, ZHANG Hou-li1, DENG Sa1, LI Xiao-yu2, FANG Rui1, CHEN Jie2, XU Yong-ping2

(1.College of Pharmacy, Dalian Medical University, Dalian 116044, China; 2.Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
Contact: XU Yong-ping

Abstract

Abstract: The objective of this study was to produce multi-valent egg yolk immunoglobulin(IgY) against mastitis and detect the content of specific IgY. IgY was produced by immunizing laying hens with the mixture of formaldehyde killed Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli, and was isolated from yolk by water dilution. Titer and content of specific IgY was detected by ELISA. The highest titer of the multi-valent antibody against Escherichia coli was 25600, that against Staphylococcus aureus and Streptococcus agalactiae was both 12800. The content of specific IgY in yolk against Escherichia coli, Staphylococcus aureus and Streptococcus agalactiae was 2.13，2.01 and 1.92 mg/mL, respectively. The varying trend of specific IgY content accorded to that of IgY titer.

Key words: egg yolk immunoglobulin; mastitis; Staphylococcus aureus; Streptococcus agalactiae; Escherichia coli

CLC Number:

S852.4⁺3

ZHEN Yu-hong;;DIAO Yun-peng;SHU Xiao-hong;ZHANG Hou-li;DENG Sa;LI Xiao-yu;FANG Rui;CHEN Jie;XU Yong-ping. Production and Content Detection of Multi-valent IgY against Cow Mastitis[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(3): 134-136.

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Production and Content Detection of Multi-valent IgY against Cow Mastitis

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