China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (8): 45-48.

• 遗传繁育 • Previous Articles     Next Articles

Effects of Neuroendocrine Factors on Expression of GLNR mRNA in MonolayerPrimary Cultured Neonatal Calf Hepatocytes in Vitro

ZHANG Yonghong1, SUN Yucheng1, LIU Guowen1, GAO Yan2,
WANG Xueying3, LI Xiaobing1, WANG Zhe1   

  1. 1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2.College of Animal Science and Veterinary Medicine, Inner Mongolia Nation University, Tongliao 028000,China; 3.College of Animal Science and Veterinary Medicine, Jilin Agricultural University ,Changchun 130118, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-20 Published:2007-08-20

Abstract: In order to investigate the regulation role of neuroendocrine factors Insulin(In), glucagon(GLN) and leptin(LEP) in hepatocyte glycometabolism and lipometabolism of dairy cows, effects of In, GLN and LEP on expression of neonatal calf glucagon receptor（GLNR）mRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.In results, the production of GLNR mRNA increased(P<0.01) along with increase of levels of In;and when glucagon in the culture media increased gradually, the production of GLNR mRNA decreased (P<0.01). Along with increase of leptin in the medium, the production of GLNR increased firstly and then decreased (P<0.01). These results demonstrated that In, GLN and LEP in the medium can directly regulate the expression of GLNR mRNA in the neonatal calf hepatocytes in vitro.

Key words: metabolite; hepatocyte; GLNR mRNA

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