海洋源β-内酰胺类抗生素降解菌的分离鉴定及全基因组测序分析

doi:10.16431/j.cnki.1671-7236.2026.02.042

Abstract

Abstract:

Objective This study aimed to isolate and screen β-lactam antibiotic-degrading bacteria，analyze their degradation activity and whole genome， and provide new ideas for repairing β-lactam antibiotic pollution in the environment. Method In this study， selective media containing penicillin potassium， amoxicillin， and ceftriaxone sodium were used to screen bacteria from seawater in Zhanjiang city in Guangdong province. The degradation rates were determined through rapid biodegradation tests. Molecular biological identification was performed on strains with degradation activity， and time-degradation curves were drawn. Degrading bacteria that degraded more than 90% of antibiotics within 120 h were selected to analyze the degradation effect at higher initial antibiotic concentrations， and the strain with the best degradation effect was screened for whole genome sequencing analysis. Result 18 strains of β-lactam antibiotic-resistant bacteria were screened from seawater in Zhanjiang， among which 5 strains could rapidly degrade β-lactam antibiotics. By drawing time-degradation curves， it was found that strains DA02 and DA03 could degrade 99.12% and 91.21% of ceftriaxone sodium with an initial concentration of 100 mg/L within 120 h， and DA02 could rapidly degrade ceftriaxone sodium with an initial concentration of 300 mg/L. The 16S rRNA gene sequencing analysis results showed that strain DA02 was Stenotrophomonas maltophilia. Whole genome sequencing identified strain DA02 with a genome size of 4 786 356 bp， a GC content of 66.58%， and a total of 4 253 predicted coding genes， 73 tRNAs， and 7 rRNAs. Multiple genes encoding β-lactamases were found， including L1_BLA， LRA-18， NmcR， PSV-1 and SRT-1. The bacterium also contained various antibiotic resistance genes， such as AAC（6'）， APH（3'）， cmlv and arr-2. It also possessed abundant carbohydrate metabolic pathways， with 91， 12 and 13 genes involved in carbon metabolism， sulfur metabolism， and nitrogen metabolism， respectively. Conclusion Highly efficient β-lactam antibiotic-degrading bacteria existed in seawater. Whole genome analysis revealed the possible degradation mechanism and bioremediation potential of ceftriaxone sodium-degrading bacterium DA02， providing a potential effective method for repairing β-lactam antibiotic pollution in the environment.

Key words: biodegradation; seawater; β-lactam antibiotics; whole genome

CLC Number:

S963.21^＋1

ZHAO Yining, PAN Ruixue, LONG Yuner, LIN Qianqian, WU Shiyun, PENG Jinju, MA Yi. Isolation， Identification and Whole Genome Sequencing Analysis of Marine-derived β-lactam Antibiotic-degrading Bacteria[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 984-994.

Figures/Tables 8

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References 42

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菌株 Strains	青霉素钾 Penicillin potassium	阿莫西林 Amoxicillin	头孢曲松钠 Ceftriaxone sodium
海水细菌Seawater bacteria	29.77±5.70	21.32±1.53	56.56±5.86
DP01	15.90±1.27	1.40±0.66	1.02±1.23
DP02	43.21±4.97	23.36±0.64	3.39±0.71
DP03	7.51±1.12	2.00±0.67	100.00±0.00
DP04	10.73±1.01	0.53±0.02	1.27±0.74
DP05	32.22±1.14	0.43±0.60	1.86±1.45
DP06	4.83±1.32	0.68±0.45	2.50±1.26
DP07	56.37±4.81	25.54±0.67	0.20±0.25
DA01	32.35±0.57	6.24±0.61	53.17±2.79
DA02	3.13±1.26	11.35±2.13	100.00±0.00
DA03	90.89±2.05	21.78±1.17	98.83±2.02
DA04	10.97±0.97	4.59±0.40	1.11±0.31
DA05	7.12±0.96	2.00±1.15	2.57±2.47
DA06	99.17±0.73	13.53±0.61	12.47±0.82
DT01	77.90±4.38	20.56±2.61	52.76±3.01
DT02	57.63±5.15	20.84±2.92	49.93±4.09
DT03	50.32±6.13	15.98±0.63	48.83±3.73
DT04	10.05±2.56	18.55±1.05	51.33±2.26
DT05	5.01±1.11	5.08±0.34	13.63±0.64

Isolation， Identification and Whole Genome Sequencing Analysis of Marine-derived β-lactam Antibiotic-degrading Bacteria

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