基因编辑技术及其在家禽抗病育种中的应用

doi:10.16431/j.cnki.1671-7236.2025.10.022

Abstract

Abstract: Gene editing,as an emerging genetic engineering technology,can precisely modify the specific gene sequences of an organism’s genome.In recent years,gene editing technology has evolved from the first-generation zinc finger nuclease (ZFN),the second-generation transcriptional activator-like effector nuclease (TALEN),to the current third-generation CRISPR-Cas9 technology,and has been successfully applied in the fields of crops and livestock.With the rapid development of science and technology and the poultry industry,the application of gene editing technology in the field of poultry disease-resistant breeding has become the focus of attention for breeders.This article systematically introduces gene editing technology and its development and application in poultry disease-resistant breeding,briefly describes the development process of three generations of gene editing technology,and expands other types of CRISPR/Cas systems.The application of new gene editing technologies represented by CRISPR-Cas9 technology in disease-resistant breeding of poultry such as chickens and ducks was analyzed,aiming to provide references for the research and application of disease-resistant breeding of poultry.Although gene editing technology shows great potential,it still faces challenges such as off-target effects,the safety of delivery systems and public acceptance.In the future,the precision of the technology can be further enhanced by developing high-fidelity Cas variants,optimizing delivery vectors (such as nanomaterials),and establishing multi-gene collaborative editing strategies.Gene editing technology will achieve industrial breakthroughs in areas such as disease-resistant breeding and medicinal protein production,and promote the dual improvement of independent innovation in the poultry breeding industry and food safety guarantee.

Key words: gene editing; CRISPR-Cas9; poultry; disease-resistant breeding

CLC Number:

WANG Chenyu, CHEN Shihao, BI Yulin, CHEN Guohong, CHANG Guobin, BAI Hao. Gene Editing Technology and Its Application in Poultry Disease-resistant Breeding[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4765-4775.

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Gene Editing Technology and Its Application in Poultry Disease-resistant Breeding

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