犬细小病毒VP2蛋白特点及病毒样颗粒疫苗制备研究进展

doi:10.16431/j.cnki.1671-7236.2023.08.026

Abstract

Abstract: Canine parvovirus disease endangers the health of the dog,the traditional vaccine can’t effectively protect dogs from viruses,looking for a new vaccine is a hotspot of current research and attention.VP2 protein is the main protective antigenic protein of Canine parvovirus (CPV),which plays an important role in determining host range and invasion process.In addition,the VP2 protein contains several important B-cell epitopes in the N and ring domains that induce the production of effective neutralizing antibodies during CPV infection.Therefore,VP2 protein is considered as the first choice of candidate antigens in the development of CPV genomic subunit vaccines.Viral-like particles (VLPs) are similar to natural viruses,but they do not have the replication function of natural viruses to cause infection in the body.Therefore,in recent years,the assembly of VP2 proteins using different expression systems has been a focus in the study of the researchers.The author focuses on the analysis of the characteristics and conformation of CPV VP2 protein.Through the analysis of the characteristics,it is known that the main component of CPV capsid contains VP2,which not only has hemagglutination activity,but also is closely related to the host range and antigenicity of CPV.Therefore,active VP2 protein is extremely important in the development of CPV genetic engineering subunit vaccine.According to conformational analysis,it is found that the Flexible loop of CPV VP2 protein has greater flexibility,which may be the molecular basis for the continuous expansion of the host range of CPV infection.At the same time,the author discusses the preparation methods and advantages and disadvantages of VLPs vaccines,in order to provide favorable references and scientific basis for the research and preparation of CPV vaccines.

Key words: Canine parvovirus (CPV); virus-like particles (VLPs); vaccine; VP2 protein

CLC Number:

S852.65⁺5

YANG Yuanru, JIAO Xue, SONG Weilin, LIU Xufeng, WANG Huiqing, LI Yuehong. Research Progress on the Characterization of Canine Parvovirus VP2 Protein and Preparation of Virus-like Particles Vaccine[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(8): 3286-3293.

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Research Progress on the Characterization of Canine Parvovirus VP2 Protein and Preparation of Virus-like Particles Vaccine

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