动物血液转录组测序的应用研究进展

doi:10.16431/j.cnki.1671-7236.2023.02.004

Abstract

Abstract: In recent years,with the popularity and cost reduction of RNA-sequencing (RNA-Seq) technology,it is increasingly used in animal science research.Several genes in blood are closely related to diseases and involved in immune,stress,metabolism and disease-related functions.For example,syntaxin 1A (STX1A) gene may be a molecular target for predicting ketosis.Blood RNA-Seq can predict blood marker related to the diagnosis of early pregnancy,such as preterm birth,recurrent fertilization failure and abortion (for example,lysophosphatidic acid receptor 3,LPAR3).The use of blood RNA-Seq to identify reliable predictors of feed efficiency is a strategy to reduce the collection of phenotypic data,which could help the field of nutritional regulatory genomics in livestock.There are markers related to growth traits and performance of livestock in blood.For example, CD48 gene is a key gene regulating the growth of beef cattle.Blood is an important line of defense of the immune system,which can transport various immune active substances and immune cells.The analysis of blood RNA-Seq provides an overview of systemic immunity. Interleukin 1 receptor type Ⅱ (IL1R2) is a major gene of immunomodulation in the wild state.Studies on the application of RNA-Seq in mammalian blood,especially in livestock,show that blood is rich in information about health status,which is expected to replace pathological tissue sampling to study the molecular characteristics of different physiological conditions and predict diseases.The genes expressed in the blood of animals with different dietary nutrition levels are different,which is helpful for the development of the field of nutritional regulatory genomics in livestock.In conclusion,the minimally invasive diagnosis of blood RNA-Seq is not only helpful to solve the molecular regulatory mechanism of different physiological states of animals,but also helpful to the application of molecular marker-assisted management of livestock in the future above these studies,which has the possibility of becoming a candidate practical biological sample.

Key words: blood RNA-Seq; immunomodulation; predict diseases; livestock; molecular marker assisted management

CLC Number:

SUN Yanyong, MA Fengying, GUO Lili, LIU Zaixia, LIU Bin, ZHANG Wenguang, LIU Yongbin. Advances on the Application of Animal Blood Transcriptome[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(2): 460-468.

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Advances on the Application of Animal Blood Transcriptome

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