RPA技术及其在动物病原检测中的应用

doi:10.16431/j.cnki.1671-7236.2021.02.008

Abstract

Abstract: Molecular detection technology is an important way of pathogen detection,identification and disease diagnosis.Recombinase polymerase amplification (RPA) is a new type of nucleic acid amplification technology.It can amplify DNA or RNA at a constant temperature with a recombinase that is capable of pairing oligonucleotide primers, single-stranded DNA binding (SSB) protein and strand-displacing polymerase.It has advantages of convenient operation,strong specificity,high sensitivity and rapid reaction,which can realize the field diagnosis of diseases.In this paper,the composition of reaction system,reaction principle,the design of primers and probes,and the detection methods of amplified products of RPA technology were introduced.The application of RPA technology in the detection of important viral,bacterial and parasitic pathogens in animals was introduced,the limitations of RPA technology were analyzed,and its development prospect was prospected.

Key words: recombinase polymerase amplification; animal pathogen; detection; application

CLC Number:

S852.6

SHI Meiyi, YANG Falong. RPA and Its Application in Detection of Animal Pathogens[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(2): 467-476.

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RPA and Its Application in Detection of Animal Pathogens

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