德州驴与泌阳驴睾丸组织mRNA与microRNA表达鉴定与分析

doi:10.16431/j.cnki.1671-7236.2019.08.022

Abstract

Abstract:

This study was aimed to explore the expression patterns of mRNA and microRNA in donkey testis, and provide genetic data for transcriptome studies of Chinese local donkeys.The mRNA and microRNA expressed in testis of Chinese domestic donkeys were analyzed by RNA-Seq and bioinformatics methods, and the differentially expressed genes and microRNA between Biyang and Dezhou donkeys were identified.The results showed that 57 837 506 (accounted for 96.34%) and 9 952 015 (accounted for 88.86%) clean reads of mRNA and Small RNA were obtained.A total of 24 751 genes and 1 074 microRNAs expressed in testis were identified.The top five expressed genes were LOC106836782, XLOC_066401, HSP90AA1, TNP1 and COF2, the top five expressed microRNAs were eca-miR-143, eca-miR-21, eca-miR-99a, eca-miR-148a and eca-miR-26a.The results revealed 102 differentially expressed genes and 2 microRNAs between Biyang and Dezhou donkeys.However, no significant function term was annotated, which suggested that there was no obvious difference of genetic expression pattern between Biyang and Dezhou donkeys.This study provided gene expression pattern and transcriptome data for donkey testis tissue and identified differentially expressed mRNA and microRNA between Biyang and Dezhou donkeys, which had scientific significance for studying genetic sources of donkeys in the future.

Key words: donkey; testis; transcriptome; differentially expressed genes; microRNA

CLC Number:

S822

HAN Haoyuan, LI Wantao, LEI Chuzhao. Identification and Analysis of mRNA and microRNA Expression of Testis Tissue in Dezhou and Biyang Donkeys[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(8): 2371-2380.

References 40

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Identification and Analysis of mRNA and microRNA Expression of Testis Tissue in Dezhou and Biyang Donkeys

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