富血小板血浆对驴感染性子宫内膜炎的体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2025.07.047

Abstract

Abstract: 【Objective】 This study aimed to investigate the anti-inflammatory and antibacterial effects of platelet rich plasma (PRP) on infectious endometritis in donkeys. 【Method】 Healthy donkey endometrium was selected as explants for in vitro culture,and 1.82×10⁷ CFU/mL Streptococcus equi subsp. zooepidemicus (S. zooepidimicus) was added to induce an infection-based endometritis explant model.The experiment was divided into 7 groups,which were mock control group (MCG),model group (MG),positive drug control group (PDCG),25% PRP and 50% PRP groups,MIX1 and MIX2 group.After the experiment,the culture medium and explants of each group were collected,and the concentration of interleukin-1 (IL-1) and IL-6 were detected in the culture medium,and the explants were examined by histology.The antibacterial and anti-inflammatory effects of PRP were evaluated by comparing bacterial concentrations in culture media,histopathological inflammatory changes in explants,and the levels of inflammatory factors. 【Result】 The PRP platelet concentration was 4.69×10¹¹/L,the platelet-derived growth factor (PDGF) concentration was 32.01 ng/mL,and the transforming growth factor β1 (TGF-β1) concentration was 49.37 ng/mL.The bacterial concentration in culture medium of MG group was extremely significantly higher than that of MCG group (P<0.01),and the bacterial concentrations in culture medium of 25% PRP,50% PRP,MIX1 and MIX2 groups were extremely significantly lower than that of MG group (P<0.01).In MCG group,the endometrium structure of the explants was intact,and few polymorphonuclear leukocytes were distributed.In MG group,the endometrial structure was severely damaged and polymorphonuclear leukocytes were infiltrated.Compared with MG group,PRP treated groups had more complete epithelial and glandular structures and less infiltration area of polymorphonuclear leukocytes.The concentration of IL-1 and IL-6 in culture medium of MG group was extremely significantly higher than that of MCG group (P<0.01).The concentrations of IL-1 and IL-6 in culture medium treated with PRP were extremely significantly lower than those in MG and PDCG groups (P<0.01). 【Conclusion】 In this study,an explants model of donkey infective endometritis induced by S. zooepidimicus was successfully constructed.PRP could significantly inhibit the growth of bacteria in explants,down-regulate the expression of inflammatory factors IL-1 and IL-6,and alleviate tissue inflammation in the explants.

Key words: platelet rich plasma (PRP); donkey; infectious endometritis; explant

CLC Number:

S852.2

LAN Xiner, LIU Sirui, CHEN Qinglin, JIANG Hui, PENG Cong, WANG Zixuan, ZHU Yiping, LI Jing. Inhibitory Effect of Platelet Rich Plasma on Infectious Endometritis in Donkeys in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3489-3495.

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Inhibitory Effect of Platelet Rich Plasma on Infectious Endometritis in Donkeys in vitro

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