China Animal Husbandry & Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (8): 2273-2280.doi: 10.16431/j.cnki.1671-7236.2019.08.010

• Physiology and Biochemistry • Previous Articles     Next Articles

Analysis of Differences in Hindgut Microbiota of Holstein Dairy Cows During Heat Stress

CHEN Shaokan1,2, LUO Hanpeng2, ZHANG Guoxing2, QIU Wenqing1,2, DONG Yixin2, WANG Yan3, HUANG Xixia1, WANG Yachun1,2   

  1. 1. College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China;
    2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    3. Beijing Sunlon Livestock Development Co., Ltd., Beijing 100176, China
  • Revised:2019-05-09 Online:2019-08-20 Published:2019-08-17

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Abstract:

The objective of this study was to analyze the differences of hindgut microbiota composition between heat stress sensitive cows and heat stress tolerant cows under heat stress condition, to find specific microbiota related to heat stress, and to provide the theoretical basis for improving thermotolerance of dairy cows via improving feeding management and genetic selection.Nineteen Chinese Holstein dairy cows which were in 1st lactation and with the similar body conditions were divided into two groups by the difference of morning and afternoon rectal temperature:Heat stress sensitive group (group H, n=10) and heat stress tolerant group (group L, n=9).The sequences of V3 and V4 region for 16S rDNA were sequenced by Illumina PE250 sequencing platform and bioinformatic methods were used to detect the differential microbiota.The results showed that:①The Shannon and Chao1 indexes in group L were higher than that in group H, but there was no significant diffference (P>0.05).②For phylum level, over 95% of the sequences in both groups of cows were assigned to Firmicutes, Bacteroidetes and Proteobacteria.For genus level, the relative abundances of YRC22 and Prevotella were significantly higher than that in group H (P<0.05), and Prevotella and YRC22 were negatively correlated to the heat stress related indexes:Afternoon rectal temperature (ART), rectal temperature difference (RTD), respiratory score (RS), rectal temperature range (RTR) and drooling score (DS).③Among the heat stress related indexes (morning rectal temperature (MRT, ART, RTD, RS, DS, RTR), RS, DS and MRT were highly correlated with the diversity of hindgut microbiota, as R2 values were top three of these six indexes in the RDA results.In conclusion, the abundance of hindgut bacteria were influenced by heat stress.Prevotella could be the hindgut microbiota related to heat stress in dairy cows.

Key words: Holstein dairy cows; heat stress; hindgut microbiota; Prevotella

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