不同断奶日龄对仔猪脾脏生长相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2016.02.014

Abstract

Abstract: In order to study the effect of different weaning age on related-growth gene expressions of spleen in piglets,24 similar body weight and healthy piglets were selected (Duroc×Landrace×Yorkshine).All piglets were randomly divided into 4 groups (14,21,28 and 35 days of age weaning groups),and each group had 6 replications including 1 piglet separately.They were slaughtered at 24 days of age and spleen samples were acquired.The relative expression of growth hormone receptor (GHR),insulin like growth factor-1 (IGF-1),insulin like growth factor-1 receptor (IGF-1R) mRNA in spleen were measured by RT-PCR.The results showed that the relative expression of GHR mRNA in 14 and 21 days of age weaning groups was significantly higher than that in 35 days of age weaning group (P<0.05);The relative expression of IGF-1 mRNA in spleen at different weaning age groups was no significant difference (P>0.05);The relative expression of IGF-1R mRNA in 35 days of age weaning group was significantly higher than that in 28 days of age weaning group (P<0.05).The results indicated that the patterns of weaning age on related growth gene expressions of spleen are different;The expressions of spleen IGF-1R mRNA in different weaning age groups were possibly effected by the spleen self regulation of IGF-1.

Key words: weaning age; piglet; spleen; gene expression; GHR; IGF-1; IGF-1R

CLC Number:

Q786

YANG Jing-jing, LI Yuan, LI Liu-an, JIN Tian-ming, JIAO Xiao-li. Effect of Different Weaning Age on Related-growth Gene Expressions of Spleen in Piglets[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(2): 383-388.

References

[1] Levast B,de Monte M,Chevaleyre C,et al.Ultra-early weaning in piglets results in low serum IgA concentration and IL17 mRNA expression[J]. Vet Immunol Immunopathol,2010,137(3-4):261-268.
[2] 周世良.浅谈断奶日龄对母猪和仔猪生产性能的影响[J].中国猪业,2014,9(1):34-36.
[3] 张影超,王希彪,崔世泉,等.肠上皮紧密连接蛋白mRNA的表达与仔猪断奶腹泻关系的研究[J].中国畜牧兽医,2014,41(9):221-224.
[4] 滕勇.早期断奶仔猪营养研究进展[J].中国畜牧兽医,2002,29(6):11-14.
[5] 冷静,朱仁俊.不同日龄断奶应激对仔猪血液生化和免疫指标的影响[J].安徽农业科学,2010,38(4):1853-1854.
[6] Poletto R,Steibel J P,Siegford J M,et al.Effects of early weaning and social isolation on the expression of glucocorticoid and mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase 1 and 2 mRNAs in the frontal cortex and hippocampus of piglets[J].Brain Res,2006,1067(1):36-42.
[7] Kanitz E,Puppe B,Tuchscherer M,et al.A single exposure to social isolation in domestic piglets activates behavioural arousal,neuroendocrine stress hormones,and stress-related gene expression in the brain[J].Physiol Behav,2009,98(1-2):176-185.
[8] Matteri R L,Dyer C J,Touchette K J,et al.Effects of weaning on somatotrophic gene expression and circulating levels of insulin-like growth factor-1 (IGF-1) and IGF-2 in pigs[J].Domest Anim Endocrinol,2000,19(4):247-259.
[9] Mathews L S,Enberg B,Norsredt G.Regulation of rat growth hormone receptor gene expression[J].J Biol Chem,1989,264(17):9905-9910.
[10] Mertani H C,Morel G.In situ gene expression of growth hormone (GH) receptor and GH binding protein in adult male rat tissues[J].Mol Cell Endocrinol,1995,109(1):47-61.
[11] Herrington J,Carter-Su C.Signaling pathways activated by the growth hormone receptor[J].Trends Endocrinol Metab,2001,12(6):252-257.
[12] 刘莉,郝福星,冒海银.四君子汤对仔猪胸腺及脾脏生长激素受体mRNA表达的影响[J].动物医学进展,2009,30(6):66-69.
[13] Zhang S J,Li X,Li L,et al.Autophagy up-regulation by early weaning in the liver,spleen and skeletal muscle of piglets[J].Br J Nutr,2011,106(2):213-217.
[14] 冷静,朱仁俊.不同日龄断奶应激对仔猪血液生化和免疫指标的影响[J].安徽农业科学,2010,38(4):1853-1854.
[15] 李留安,王凤云,杨晶晶,等.断奶日龄对仔猪脾脏、胸腺和胰腺抗氧化功能的影响[J].动物营养学报,2014,26(1):74-80.
[16] 王锐,赵跃,张永云,等.猪胰岛素样生长因子-Ⅰ的克隆及生物信息学分析[J].西南农业学报,2014,27(2):864-869.
[17] 石子刚.生长轴激素在猪脾脏中表达及其发育性变化[D].南京:南京农业大学,2002.

Effect of Different Weaning Age on Related-growth Gene Expressions of Spleen in Piglets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	RUAN Shihui, LIU Chunyan, WEI Yangyang, HE Yiyi, WU Qiwen, XIONG Yunxia, YANG Xuefen, WANG Li, YI Hongbo. Effect of PDCoV on the Extracellular Matrix of the Intestine and Its Dynamic Changes in Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3297-3307.
[2]	YU Miao, LI Yanjiao, ZHAO Yanling, CHAO Zhe, WANG Feng, LI Menghan, JIANG Zhiqiang, ZHANG Ting, XUE Xinyu, REN Zili, SUN Ruiping. Effects of Weaning Stress on Intestinal Morphology, Antioxidant Capacity and Nrf2 Signaling Pathway of Wuzhishan Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2045-2055.
[3]	WANG Yong, NA Risu, LIANG Jianyong, LIU Yang, LI Jiuyue, GAO Yuan, TIAN Feng, XUE Shuyuan. Effects of Quercetin on Follicular Development,Follicular Fluid Microenvironment and Ovarian Steroidogenesis Related Gene Expression in Mongolian Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1150-1158.
[4]	LIU Shiyang, ZHANG Ziqiang, WANG Shishi, NIU Bingyu, KONG Dejing, ZHANG Zhikai, LIU Yumei. Protective Effect of Selenomethionine on Spleen Injury Induced by Aflatoxin B₁ in Rabbits [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3828-3836.
[5]	WU Yuxing, ZOU Weili, CHENG Zixin, MO Yupeng, ZHANG Lingyuan, JI Wantong, CHEN Siyu, ZHENG Haodong, MA Runwen, LI Xun, LI Maoning, WANG Xiaoye. Isolation, Identification and Pathogenicity Analysis of a Strain of Swine-derived Clostridium perfringens Type A [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2697-2706.
[6]	LIU Dingyu, LIU Baoling, HE Zhenwen, LUO Qin, QIAO Changhong, CHEN Xiangyu, WANG Xiaohu, XIANG Hua, WANG Gang, TAN Chen, CAI Rujian. Isolation,Identification and Pathogenicity Analysis of a Strain of Streptococcus suis Causing Meningitis in Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2143-2153.
[7]	HUANG Yongpeng, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Gene Expression Change and Its Correlation Analysis with Meat Quality During Postmortem Pork Aging [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1573-1581.
[8]	YANG Zhikang, YIN Yunju, XU Weiwei, CHEN Guang, ZHENG Mengli, YAN Zhaoming, LIU Yating, ZHANG Guanfeng, PENG Jie, JIANG Huiqiong, HU Youjun, ZHAO Xiaonan, CHEN Qinghua. Effects of Organic Acid Combined with Essential Oil,Tannic Acid and Compound Enzyme Preparation on Growth Performance,Serum Biochemical Indexes and Intestinal Heath of Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 978-989.
[9]	ZHAO Xiaonan, HU Youjun, CHENG Huangzuo, WEN Wei, LU Xinfang, HE Zishi, CHEN Jiemei, E Xiaodi, HE Qiantai, ZOU Tiande. Effects of Different Types of Benzoic Acid on Growth Performance, Serum Biochemical Indices and Intestinal Flora of Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 1031-1040.
[10]	JIANG Bingyu, CHEN Ning, CUI Yanan, ZHU Kaiqing, JIANG Pengxin, WANG Xutao, LI Yan. Investigation of the Mechanism of Sihuang Antidiarrheal Granules in the Treatment of Piglets Diarrhea Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 1308-1319.
[11]	WEI Haibo, LIU Yingchun, SUN Shuguang, LIU Xiwu, ZHU Guihua, DAI Zhenghao, LIU Zongzheng, WEN Fengyun. Effects of Dietary Probiotics and Acid Preparations on Growth Performance and Intestinal Flora of Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(12): 5302-5316.
[12]	PAN Chenglin, SONG Tongxing, ZHANG Xiaojun, LOU Fangfang, HU Xujin, TU Pingguang, XIAO Yingping, LYU Wentao. Study on the Structure and Function of the Archaea Community and Its Correlation with Diarrhea of Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4755-4764.
[13]	LI Hongyi, YANG Xuefen, LIN Zesen, WU Xiaofei, LYU Qixin, YU Jiali, ZHANG Mao. Effect of Lactobacillus reuteri Replacing Antibiotics on the Expression of Intestinal miRNA in Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(1): 74-85.
[14]	LIU Chunyan, TANG Qingsong, YANG Bijing, WU Qiwen, XIONG Yunxia, HUANG Yanna, YANG Xuefen, WANG Li, JIANG Zongyong, YI Hongbo. Effects of Enterotoxigenic Escherichia coli on Intestinal Morphology, Tight Junction Protein and Extracellular Matrix of Weaned Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(7): 2697-2706.
[15]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(6): 2380-2387.