关键词: 中药; 参姜止痢合剂; 免疫调节; 淋巴细胞

Abstract: 【Objective】 To provide experimental basis for its pharmacological research and clinical medication,the regulatory effect of Shenjiang Zhili mixture (SJZL) on mouse immune function in vivo was investigated. 【Method】 Fourty-eight BALB/c mice were randomly divided into low- (5 g/kg),middle- (10 g/kg) and high-dose group (20 g/kg) of SJZL and blank control group,with 12 mice in each group.Mice in each drug group were given intragastric administration once a day.And mice in blank control group were regularly administered with equivalent saline every day for 6 days.After 3 and 6 days of administration,6 mice were randomly selected from each group.Spleen index and thymus index were calculated after weighing.The splenic cell suspension was prepared and the lymphocyte proliferation rate was determined by the CCK-8 kit.Mouse peritoneal macrophage suspension was prepared,and the phagocytic ability of mouse peritoneal macrophages was detected by neutral red method.Blood was collected from the mouse eyeball,and the levels of interleukin-2 (IL-2),IL-6,tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in serum were detected by ELISA Kit. 【Result】 The clinical index including mental state,feed intake,water intake,stool and urine of mice in each group were normal.Compared with blank control group,there were no significant differences in weight,spleen index and thymus index in each treatment group (P＞0.05),the proliferation rate of T cells in SJZL high-dose group were extremely significantly increased (P＜0.01),the proliferation rate of B cells in SJZL high-dose group was significantly or extremely significantly increased (P＜0.05 or P＜0.01),the phagocytosis rate and metabolism of mouse peritoneal macrophages in high-dose group were significantly or extremely significantly increased (P＜0.05 or P＜0.01).After 6 days of administration,the serum levels of IL-2,IL-6 and IFN-γ in high-dose group were significantly or extremely significantly higher than those in blank control group (P＜0.05 or P＜0.01).In addition,the proliferation rate of T cells and B cells,the phagocytosis and metabolism of peritoneal macrophages,and the levels of IL-2,IL-6 and IFN-γ were increased with increasing days of treatment. 【Conclusion】 Under the conditions of this experiment,10 and 20 g/kg of SJZL could promote the proliferation of T cells and B cells,enhance the phagocytosis and metabolism of macrophages,and increase the secretion of related cytokines in mouse serum,thereby enhancing immunity in mice.

Key words: traditional Chinese medicine; Shenjiang Zhili mixture; immune regulation; lymphocytes