9型猪链球菌的分离鉴定及其生物学特性研究

doi:10.16431/j.cnki.1671-7236.2025.10.029

中国畜牧兽医 ›› 2025, Vol. 52 ›› Issue (10): 4844-4853.doi: 10.16431/j.cnki.1671-7236.2025.10.029

• 预防兽医 • 上一篇

9型猪链球菌的分离鉴定及其生物学特性研究

吴建冬^1,2, 康桦华¹, 翟少伦¹, 杨冬霞¹, 杨南玲¹, 勾红潮¹, 贾爱卿³, 李小军⁴, 李春玲¹, 李艳¹

1. 广东省农业科学院动物卫生研究所, 广东省畜禽疫病防治研究重点实验室, 农业农村部兽用药物与诊断技术广东科学观测实验站, 广州 510640;
2. 佛山大学动物科技学院, 佛山 528225;
3. 广东海大畜牧兽医研究院有限公司, 番禺 511400;
4. 东莞市动物疫病预防控制中心, 东莞 523086

收稿日期:2024-12-23 发布日期:2025-09-30
通讯作者: 李春玲, 李艳 E-mail:707989782@qq.com;371273724@qq.com
作者简介:吴建冬，E-mail：3080233774@qq.com;康桦华，E-mail：534011491@qq.com。
基金资助:
广东省自然科学基金面上项目(2023A1515012206、2025A1515010999)；广东省科技计划项目(2023B0202010009)；广东省农业科学院粤东黑猪健康养殖专家工作站建设项目(2024工作站19)；农业领域为单元的广东省现代农业产业共性关键技术研发创新团队建设项目(生物安全与防控(动物方向)共性关键技术)(2024CXTD15)；国家重点研发项目“大型养猪集团公司重大疫病综合防控与净化推广”(2024YFD1800505)

Isolation and Identification of Streptococcus suis Serotype 9 and Its Biological Characteristics Analysis

WU Jiandong^1,2, KANG Huahua¹, ZHAI Shaolun¹, YANG Dongxia¹, YANG Nanling¹, GOU Hongchao¹, JIA Aiqing³, LI Xiaojun⁴, LI Chunling¹, LI Yan¹

1. Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Animal Science and Technology, Foshan University, Foshan 528225, China;
3. Guangdong Haid Animal Husbandry & Veterinary Research Institute Co., Ltd., Panyu 511400, China;
4. Dongguan Animal Disease Prevention and Control Center, Dongguan 523086, China

Received:2024-12-23 Published:2025-09-30

摘要/Abstract

摘要： 【目的】了解9型猪链球菌广东地区分离株生物学特性及致病性，为其所致疾病的防治提供参考。【方法】从广东某猪场一猪繁殖与呼吸综合征发病猪脾脏中取样分离培养，通过形态学观察、革兰染色镜检、生化试验、16S rRNA基因测序及血清型鉴定对分离菌进行鉴定，同时通过毒力基因检测、药敏试验及小鼠感染试验，分析分离菌株生物学特性及致病性。【结果】分离菌株在血平板上生长出灰白色、湿润、表面光滑、边缘整齐的圆形菌落，呈α型溶血特征，革兰染色镜检为紫色，圆形链状排列。分离菌16S rRNA基因测序结果与NCBI上已发布的猪链球菌菌株序列相似性达99%以上。血清型鉴定结果显示，分离菌株为9型猪链球菌。毒力基因检测结果显示，分离菌株毒力基因表型为luxS^-/ccpA⁺/srtA⁺/mrp^-/gdh⁺/gapdh⁺/fbps⁺/epf^-/orf2⁺/sly^-。分离菌株对大观霉素、林可霉素、多西环素、四环素、磺胺二甲异噁唑5种抗菌药耐药。小鼠致病性试验结果显示，该分离菌株对小鼠的半数致死量(LD₅₀)为1×10¹⁰ CFU，感染小鼠可导致肝脏、肾脏、脾脏、肺脏、心脏和脑等脏器不同程度病变。【结论】本研究分离到1株9型猪链球菌，其携带多种毒力基因，对多种抗菌药耐药，感染小鼠可致组织不同程度损伤。研究结果可为猪链球菌流行分析及防控提供参考依据。

关键词: 9型猪链球菌; 分离鉴定; 生物学特性; 致病性

Abstract: 【Objective】 The purpose of this study was to understand the biological characteristics and pathogenicity of the isolates of Streptococcus suis serotype 9 in Guangdong,and provide a reference for the prevention and treatment of the disease caused by it.【Method】 In this experiment,bacterial isolation and culture were carried out with spleen samples from a pig with porcine reproductive and respiratory syndrome (PRRS) in a pig farm in Guangdong,and the isolated strain was identified by morphological observation,Gram staining microscopy,biochemical tests,16S rRNA gene sequencing and serotype identification.The biological characteristics and pathogenicity of the isolated strain were analysed by virulence gene detection,drug sensitivity test and mouse infection test.【Result】 The isolated strain showed rounded colonies of greyish-white,moist,smooth,and neat edges on blood plate,characteristic of alpha-haemolysis.Gram staining microscopic examination showed purple and was arranged in a circular chain pattern.Sequencing results of the 16S rRNA gene of the isolate showed more than 99% similarity with the published strains of Streptococcus suis on NCBI,and serotyping results showed that the isolate was Streptococcus suis serotype 9.The virulence gene phenotype of the isolated strain was luxS^-/ccpA⁺/srtA⁺/mrp^-/gdh⁺/gapdh⁺/fbps⁺/epf^-/orf2⁺/sly^-.The isolated strain was resistant to five antibiotics:Macrolide,lincomycin,doxycycline,tetracycline and sulfadimethoxazole.The results of pathogenicity test showed that the LD₅₀ of the isolate to mice was 1×10¹⁰ CFU,and the infected mice exhibited different degrees of lesions in organs,such as liver,kidney,spleen,lung,heart and brain.【Conclusion】 In this study,a strain of Streptococcus suis serotype 9,which carried multiple virulence genes was isolated,it was resistant to a variety of antibiotics,and could cause different degrees of tissue damage in infected mice.The results of this study could provide a reference basis for the prevalence analysis and prevention and control of Streptococcus suis infection.

Key words: Streptococcus suis serotype 9; isolation and identification; biological characteristics; pathogenicity

中图分类号:

S852.61

吴建冬, 康桦华, 翟少伦, 杨冬霞, 杨南玲, 勾红潮, 贾爱卿, 李小军, 李春玲, 李艳. 9型猪链球菌的分离鉴定及其生物学特性研究[J]. 中国畜牧兽医, 2025, 52(10): 4844-4853.

WU Jiandong, KANG Huahua, ZHAI Shaolun, YANG Dongxia, YANG Nanling, GOU Hongchao, JIA Aiqing, LI Xiaojun, LI Chunling, LI Yan. Isolation and Identification of Streptococcus suis Serotype 9 and Its Biological Characteristics Analysis[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4844-4853.

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9型猪链球菌的分离鉴定及其生物学特性研究

Isolation and Identification of Streptococcus suis Serotype 9 and Its Biological Characteristics Analysis

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