1株黑熊源赫尔曼亚特兰大杆菌的分离鉴定及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2025.09.030

摘要/Abstract

摘要： 【目的】从黑熊粪便中筛选具有畜禽环境修复潜能的菌株，以期为畜禽微生态制剂的开发提供候选菌株。【方法】采集新鲜黑熊粪便，采用脱硫菌选择性培养基进行菌株初筛，分离纯化后通过菌落形态和16S rDNA测序进行鉴定，并对分离株的生长特性、药物敏感性进行分析，进一步测定分离株的去除硫化氢(H₂S)能力、钝化六价铬(Cr(Ⅵ))能力、促植物生长能力以及产酶能力，多方面表征菌株的生物学特性。【结果】分离筛选获得1株赫尔曼亚特兰大杆菌，将其命名为M1633-2DR，该分离株在MHA固体培养基上呈黄色、圆形、边缘整齐菌落，表面湿润；革兰染色镜检可见阴性短杆菌。分离株在MHB液体培养基中培养1 h后进入对数生长期，16 h后进入稳定期。分离株对舒巴坦、阿米卡星等11种抗菌药物均表现敏感，对氨苄西林表现耐药。脱硫试验结果显示，分离株在48 h内对160 mg/L硫离子(S^2-)的去除率达100%，对320、480和560 mg/L S^2-的去除率分别为62.23%、49.58%和42.44%。分离株的Cr(Ⅵ)耐受浓度可达3 200 mg/L，在96 h内对50 mg/L Cr(Ⅵ)的还原率为100%，对150和250 mg/L Cr(Ⅵ)在168 h内的还原率分别为48.23%和37.78%。进一步研究发现该菌株具有解磷(829.28 mg/L)、固氮(9.94 μg/mL)、产吲哚乙酸(43.91 mg/L)和产蛋白酶(935.80 U/mL)能力。【结论】本研究分离获得1株赫尔曼亚特兰大杆菌，其在H₂S脱除、Cr(Ⅵ)还原、植物促生长和蛋白酶生产等方面均具有显著优势，可作为畜牧业环境修复和农业微生物制剂开发的候选菌株。

关键词: 赫尔曼亚特兰大杆菌; 硫化氢; 六价铬; 生物学功能; 蛋白酶

Abstract: 【Objective】 The purpose of this experiment was to screen strains with potential for livestock and poultry environmental restoration from Black bear feces,providing candidate strains for the development of livestock and poultry microecological preparations. 【Method】 Fresh Black bear feces were collected,and desulfurizing bacteria were initially screened using a selective medium.After isolation and purification,strains were identified via colony morphology and 16S rDNA sequencing.Growth characteristics and antimicrobial susceptibility of the isolated strain were analyzed.The biological properties were comprehensively characterized by evaluating its capacity for H₂S removal,Cr(Ⅵ) passivation,plant growth promotion,and enzyme production. 【Result】 One strain of Atlantibacter hermanii was successfully isolated，and it was named M1633-2DR.On MHA solid medium,it formed yellow,circular colonies with smooth edges and a moist surface.Gram staining revealed Gram-negative,short bacilli.In MHB liquid medium,the strain entered the logarithmic growth phase at 1 h and the stationary phase at 16 h.The isolate was sensitive to 11 kinds of antibacterial drugs such as sulbactam and amikacin,and resistant to ampicillin.Desulfurization tests results showed that the removal rate of 160 mg/L S^2- by the isolate reached 100% within 48 h,and the removal rates of 320,480 and 560 mg/L S^2- were 62.23%,49.58% and 42.44%, respectively.The tolerance concentration of Cr(Ⅵ) of the isolate could reach 3 200 mg/L.The reduction rate of 50 mg/L Cr(Ⅵ) within 96 h was 100%,and the reduction rates of 150 and 250 mg/L Cr(Ⅵ) within 168 h were 48.23% and 37.78%,respectively.Further studies had found that this strain had the abilities of phosphorus solubilization (829.28 mg/L),nitrogen fixation (9.94 μg/mL),indoleacetic acid production (43.91 mg/L) and protease production (935.80 U/mL). 【Conclusion】 In this study,one strain of Atlantibacter hermanii was isolated and obtained.It had significant advantages in H₂S removal,Cr(Ⅵ) reduction,plant growth promotion and protease production，and could be used as a candidate strain for environmental restoration in animal husbandry and the development of agricultural microbial preparations.

Key words: Atlantibacter hermannii; H₂S; Cr(Ⅵ); biologicalfunction; protease

中图分类号:

S852.61

张梦娜, 徐莉莉, 杨雅君, 刘荣徽, 韦燕文, 王倩, 谭镇炜, 吕红珍, 张琳, 高敏, 王承民, 孙永学. 1株黑熊源赫尔曼亚特兰大杆菌的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2025, 52(9): 4334-4345.

ZHANG Mengna, XU Lili, YANG Yajun, LIU Ronghui, WEI Yanwen, WANG Qian, TAN Zhenwei, LYU Hongzhen, ZHANG Lin, GAO Min, WANG Chengmin, SUN Yongxue. Isolation,Identification and Biological Characteristics Analysis of a Strain of Atlantibacter hermannii Derived from Black Bear[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4334-4345.

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