睾丸支持细胞的功能、分离纯化及鉴定研究进展

doi:10.16431/j.cnki.1671-7236.2021.08.027

摘要/Abstract

摘要： 支持细胞对维持精子形成过程中的微环境起决定作用，它可以通过分泌功能、细胞间连接形成的血睾屏障功能以及吞噬功能等来促进精子的形成过程，其发育异常会导致不同程度的雄性生殖缺陷。基于支持细胞在雄性动物生殖过程中的作用，体外培养高纯度支持细胞可成为研究睾丸两大核心功能-精子发生和性激素分泌功能相关调节机制重要的细胞模型。此外，体外培养睾丸支持细胞也可作为生殖毒理学等新兴热点领域的细胞模型，为评估和研究环境因素对雄性生殖的影响提供便利。因此，作者系统地归纳、总结了目前关于动物支持细胞生物功能的研究及常用的体外分离纯化、培养及鉴定方法，以期为利用动物支持细胞开展雄性生殖领域的研究提供参考。

关键词: 支持细胞; 功能; 分离纯化; 体外培养; 鉴定

Abstract: Sertoli cells play a decisive role in maintaining the microenvironment in the process of spermatogenesis.They can promote the process of spermatogenesis through secretion function, blood testis barrier function formed by intercellular connection and phagocytosis, abnormal development of them will lead to different degrees of male reproductive defects.Based on the role of Sertoli cells in the reproductive process of male animals, highly purified Sertoli cells cultured in vitro can be an important cell model to study the two core functions of testis spermatogenesis and sex hormone secretion.In addition, purified Sertoli cells cultured in vitro can also be used as a cell model in toxicology and other newly developing hot fields to provide convenience for assessing and researching on the effects of environmental factors on male reproduction.In this article, the current research progress on the function of Sertoli cell and its commonly methods of in vitro isolation, purification, culture and characterization were systematically summarized to provide references for the use of animal Sertoli cells in the studies of male reproduction.

Key words: Sertoli cells; function; isolation and purification; in vitro culture; identification

中图分类号:

S827

伊敏娜, 才文道力玛, 陶力, 王希生, 神英超, 杜明, 任宏, 芒来, 格日乐其木格. 睾丸支持细胞的功能、分离纯化及鉴定研究进展[J]. 中国畜牧兽医, 2021, 48(8): 2947-2956.

YI Minna, Ulaangerel Tseweendolmaa, Toli Davshilt, WANG Xisheng, SHEN Yingchao, DU Ming, REN Hong, MANG Lai, BOU Gerelchimeg. Research Progress on Function,Isolation,Purification and Identification of Sertoli Cells[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(8): 2947-2956.

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