杜寒绵羊多胎性能候选基因BMPR-1B和BMP15的研究

doi:10.16431/j.cnki.1671-7236.2018.05.024

摘要/Abstract

摘要：

为了从分子水平探讨杜寒绵羊的多胎机制，本试验在山西省某养殖场采集了87只经产杜寒母绵羊的耳组织，选取骨形态发生蛋白15 （bone morphogenetic protein 15，BMP15）和骨形态发生蛋白受体1B （bone morphogenetic protein receptor 1B， BMPR-1B）为候选基因，采用PCR-SSCP、PCR-RFLP法，结合母羊产羔数与所产羊羔初生重，分析其与杜寒绵羊多胎性能的相关性。结果显示，杜寒绵羊的BMPR-1B基因在第746位碱基处发生了A→G突变，检测到3种基因型：AA、AG和GG，A等位基因频率（0.5230）略高于G等位基因（0.4770），A为优势等位基因；AG基因型频率（0.5172）高于GG（0.2184）和AA（0.2644）基因型，AG为优势基因型。χ²适合性检验显示该位点处于Hardy-Weinberg平衡状态；BMPR-1B基因第864位碱基未发生突变。杜寒绵羊的BMP15基因不存在V31D和S300G位点突变。在该群体中，BMPR-1B基因A746G位点GG、AG基因型个体的产羔数极显著高于AA基因型个体（P<0.01），羔羊初生重在3种基因型间差异不显著（P>0.05）。综上所述，BMPR-1B基因是影响杜寒绵羊繁殖性能的一个主效基因，可以作为分子标记对杜寒绵羊进行辅助育种，初步排除BMP15基因突变对杜寒绵羊多胎性能影响的可能性。

关键词: 杜寒绵羊; 多胎性能; BMPR-1B基因; BMP15基因

Abstract:

In order to investigate the mechanism of prolificacy in Duhan sheep at molecular level,the ear tissues of 87 ewes were collected from multiparous Duhan ewes in Shanxi province.The BMP15 and BMPR-1B genes were selected as candidate genes,and its relationship with prolificacy,which was associated with the litter size and the primary weight of lambs,was analyzed by the methods of PCR-SSCP and PCR-RFLP.The results showed that A→G mutation occurred in Duhan sheep at 746 bp of the coding region of BMPR-1B gene, at the same time three genotypes were detected:AA,AG and GG,the genotype frequency of AG was 0.5172, higher than that of GG (0.2184) and AA (0.2644),the allele frequency of A (0.5230) was slightly higher than G(0.4770) with A and AG was the dominance allele and genotype,respectively.And the site was in Hardy-Weinberg equlilbrium in Duhan sheep.There was no mutation occurred at 864 bp and also no mutation occurred at V31D and S300G sites in BMP15 gene.The number of lambs of GG and AG genotypes were extremely significantly higher than AA genotype (P<0.01) in this population,but there was no distinct difference between three genotypes in the primary weight of lamb(P>0.05).It was concluded that the BMPR-1B gene was a major gene affected reproductive traits in Duhan sheep, which could be used as a molecular marker to perform molecular assisted selection (MAS) in Duhan sheep.The influence of BMP15 gene mutation affecting prolific ability could be preliminarily excluded in Duhan sheep.

Key words: Duhan sheep; fecundity trait; BMPR-1B gene; BMP15 gene

中图分类号:

S813

郭慧慧, 李俊, 罗惠娣, 牛晋国. 杜寒绵羊多胎性能候选基因BMPR-1B和BMP15的研究[J]. 《中国畜牧兽医》, 2018, 45(5): 1312-1318.

GUO Huihui, LI Jun, LUO Huidi, NIU Jinguo. Study of BMPR-1B and BMP15 as Candidate Genes for Prolificacy of Duhan Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(5): 1312-1318.

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