不同浓度褪黑素刺激对内蒙古绒山羊毛囊干细胞增殖活性的影响

doi:10.16431/j.cnki.1671-7236.2016.02.022

›› 2016, Vol. 43 ›› Issue (2): 438-443.doi: 10.16431/j.cnki.1671-7236.2016.02.022

不同浓度褪黑素刺激对内蒙古绒山羊毛囊干细胞增殖活性的影响

曹春花^1,2, 高峰³, 王宏^1,2, 王亚娟^1,2, 刘迎春^1,2, 周欢敏^1,2

1. 内蒙古农业大学生命科学学院, 呼和浩特 010018;
2. 内蒙古自治区生物制造重点实验室, 呼和浩特 010018;
3. 内蒙古农业大学动物科学学院, 呼和浩特 010018

收稿日期:2015-06-25 出版日期:2016-02-20 发布日期:2016-03-02
通讯作者: 刘迎春 E-mail:liuyingchun1994@sina.com
作者简介:曹春花(1989-),女,黑龙江齐齐哈尔人,硕士生,研究方向:干细胞信号转导;E-mail:13191414365@163.com
基金资助:
国家自然科学基金(30901016、31160445)

Effect of Different Concentrations of Melatonin on Proliferative Activity of Hair Follicle Stem Cells in Inner Mongolia Cashmere Goat

CAO Chun-hua^1,2, GAO Feng³, WANG Hong^1,2, WANG Ya-juan^1,2, LIU Ying-chun^1,2, ZHOU Huan-min^1,2

1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Inner Mongolia Autonomous Region Biological Manufacturing, Hohhot 010018, China;
3. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2015-06-25 Online:2016-02-20 Published:2016-03-02

摘要/Abstract

摘要： 试验旨在探究不同浓度褪黑素刺激对内蒙古绒山毛囊干细胞增殖活性的影响.采用0 (对照组)、100、300、500、700 pg/mL 5个浓度梯度分别对内蒙古绒山羊毛囊干细胞连续刺激5 d,MTT法分别检测各组细胞活性.结果表明,不同浓度褪黑素刺激2 d后,相较于对照组 (0 pg/mL),褪黑素刺激组未见成团细胞出现,开始出现长梭形细胞且细胞生长不再均一,各刺激组均表现出较高的增殖活性;刺激4 d后,500 pg/mL褪黑素刺激组毛囊干细胞增殖活性显著高于0、100 pg/mL (P<0.01;P<0.05),但与700 pg/mL褪黑素刺激组差异不显著 (P>0.05).因此,500 pg/mL是内蒙古绒山羊毛囊干细胞增殖的最适浓度.

关键词: 内蒙古绒山羊; 毛囊干细胞; 褪黑素; 细胞增殖

Abstract: This study aimed to investigate the effect of different concentrations of melatonin on proliferative activity of hair follicle stem cells in Inner Mongolia cashmere goat.The five levels melatonin including 0,100,300,500 and 700 pg/mL were designed to stimulate the hair follicle stem cells in Inner Mongolia cashmere goat,the MTT method was used to detect cell proliferation activity for 5 days.The result showed that 2 days later,the groups stimulated by melatonin did not cobble-stone arrangement and began to appear spindle cell and cell growth was not uniform compared with the control group (0 pg/mL),the groups showed obvious effect on cell proliferation relatively.4 days later,the cell proliferation of groups stimulated by 500 pg/mL melatonin were significantly greater than that of 0,100 pg/mL (P<0.01;P<0.05),but there was no significant difference with 700 pg/mL melatonin stimulation group (P>0.05).The optimal melatonin concentration of hair follicle stem cell proliferation was 500 pg/mL in Inner Mongolia cashmere goat.

Key words: Inner Mongolia cashmere goat; hair follicle stem cells; melatonin; cell proliferation

中图分类号:

S827.9⁺1

曹春花, 高峰, 王宏, 王亚娟, 刘迎春, 周欢敏. 不同浓度褪黑素刺激对内蒙古绒山羊毛囊干细胞增殖活性的影响[J]. , 2016, 43(2): 438-443.

CAO Chun-hua, GAO Feng, WANG Hong, WANG Ya-juan, LIU Ying-chun, ZHOU Huan-min. Effect of Different Concentrations of Melatonin on Proliferative Activity of Hair Follicle Stem Cells in Inner Mongolia Cashmere Goat[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(2): 438-443.

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不同浓度褪黑素刺激对内蒙古绒山羊毛囊干细胞增殖活性的影响

Effect of Different Concentrations of Melatonin on Proliferative Activity of Hair Follicle Stem Cells in Inner Mongolia Cashmere Goat

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