金黄色葡萄球菌小菌落突变株感染小鼠乳房炎模型的建立

doi:10.16431/j.cnki.1671-7236.2015.07.035

›› 2015, Vol. 42 ›› Issue (7): 1865-1870.doi: 10.16431/j.cnki.1671-7236.2015.07.035

金黄色葡萄球菌小菌落突变株感染小鼠乳房炎模型的建立

朱立力, 王奇惠, 时永强, 曲伟杰

云南农业大学动物科学技术学院, 昆明 650201

收稿日期:2014-12-24 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 曲伟杰 E-mail:quwj2008@163.com
作者简介:朱立力(1989-),男,云南红河人,硕士,研究方向:动物营养代谢病,E-mail:ynzhulili@163.com;王奇惠(1990-),女,山东烟台人,硕士,研究方向:动物营养代谢病,E-mail:wqhlgc@126.com
基金资助:
国家自然科学基金(31260629)

Estabishment of Mastitis Model in Mouse by Artificial Infection of Staphylococcus aureus Small Colony Variants

ZHU Li-li, WANG Qi-hui, SHI Yong-qiang, QU Wei-jie

College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2014-12-24 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 为了建立由金黄色葡萄球菌小菌落突变株(small colony variants,SCVs)诱发感染乳房炎动物模型,本试验取40只分娩6~8 d的BALB/c小鼠,随机分成8组(n=5),分别为阴性对照、生理盐水组和不同剂量金黄色葡萄球菌SCVs及金黄色葡萄球菌质控菌株(1.0×10³、1.0×10⁴、1.0×10⁵ CFU/mL)试验组,对生理盐水组及各试验组小鼠第4对乳腺注射生理盐水和对应剂量的菌液(50 mL/只),注射后24 h解剖观察病理变化,一侧乳头制作石蜡切片,另一侧研磨后用ELISA检测试剂盒检测组织匀浆上清中的TNF-α含量。结果显示,注射菌液的试验组,小鼠均出现不同程度的临床症状,乳腺出现不同程度的炎性症状和病理变化。同一注射剂量下,金黄色葡萄球菌质控菌株较金黄色葡萄球菌 SCVs病理变化严重,通过SPSS等软件对试验数据进行统计分析后得出,高浓度处理组金黄色葡萄球菌质控菌株的TNF-α表达量极显著高于金黄色葡萄球菌SCVs(P<0.01)。结果表明,金黄色葡萄球菌及其SCVs均可用来建立小鼠乳房炎模型,且金黄色葡萄球菌SCVs引起的情况较其正常株轻微,这一结果为由金黄色葡萄球菌SCVs引起的奶牛慢性乳房炎的预防和控制及其致病机制的研究提供了新的材料和有益的探索,为SCVs与慢性乳房炎更深层次关系的研究奠定基础。

关键词: 金黄色葡萄球菌小菌落突变株; 小鼠; 乳房炎模型

Abstract: In order to establish the mouse mastitis model induced by Staphylococcus aureus small colony variants (SCVs),40 BALB/c mice 6 to 8 days postpartum were randomly divided into eight groups,negative control group,physiologic saline group and six treated groups with different doses (1.0×10³,1.0×10⁴ and 1.0×10⁵ CFU/mL) of Staphylococcus aureus SCVs or Staphylococcus aureus quality control strains.50 μL physiologic saline and Staphylococcus aureus liquid were injected into the forth mammary glands in physiologic saline group and the six treated groups,respectively.All the mice were sacrificed 24 h after treatment.One side of the forth mammary glands was used to make pathological section,the other side was used to detect the concentration of TNF-α in the supernatant.The results showed that mice had different degrees of clinical symptoms in the treated groups,their mammary glands appeared different degrees of inflammatory symptoms and pathological changes.Under the same injected dose,the Staphylococcus aureus quality control strains group pathologic changed more severe than the Staphylococcus aureus SCVs group.The experimental data had been statistically analyzed by using SPSS software,the result showed that the expression of TNF-α of the Staphylococcus aureus quality control strains group were extremely significantly higher than that of Staphylococcus aureus SCVs group with high dose (P<0.01).The results indicated that Staphylococcus aureus and its SCVs could be used to establish the mice mastitis model,and Staphylococcus aureus SCVs caused relatively minor inflammation than the normal strains.The results provided a new research materials and meaningful exploration to research the prevention and control of chronic mastitis cows and its pathogenic mechanism caused by Staphylococcus aureus SCVs,and laid the foundation for studying the deeper relationship between Staphylococcus aureus SCVs and chronic mastitis.

Key words: Staphylococcus aureus small colony variants; mouse; mastitis model

中图分类号:

R378

朱立力, 王奇惠, 时永强, 曲伟杰. 金黄色葡萄球菌小菌落突变株感染小鼠乳房炎模型的建立[J]. , 2015, 42(7): 1865-1870.

ZHU Li-li, WANG Qi-hui, SHI Yong-qiang, QU Wei-jie. Estabishment of Mastitis Model in Mouse by Artificial Infection of Staphylococcus aureus Small Colony Variants[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(7): 1865-1870.

参考文献

[1] Proctor R A,van Langevelde P,Kristjansson M,et al.Persistent and relapsing infections associated with small colony variants of Staphylococcus aureus[J].Clinical Infectious Diseases,1995,20(1):95-102.
[2] Atalla H,Gyles C,Jacob C L,et al.Characterization of a Staphylococcus aureus small colony variant(SCV) associated with persistent bovine mastitis[J]. Foodborne Pathog Dis,2008,5(6):785-799.
[3] Balwit J M,van Langevelde P,Vann J M,et al.Gentamicin-resistant menadione and hemin auxotrophic Staphylococcus aureus persist within cultured endothelial cells[J]. The Journal of Infectious Diseases,1994,170(4):1033-1037.
[4] Besier S,Ludwig A,Ohlsen K,et al.Molecular analysis of the thymidine-auxotrophic small colony variant phenotype of Staphylococcus aureus[J].Int J Med Microbiol,2007,297(4):217-225.
[5] 朱立力,孙玉林,于馨,等.胸腺嘧啶依赖性金黄色葡萄球菌的分离鉴定[J].中国畜牧兽医,2014,41(10):122-127.
[6] Rambeaud M,Almeida R A,Pighetti G M,et al.Dynamics of leukocytes and cytokines during experimentally induced Streptococcus uberis mastitis[J].Vet Immunol Immunopathol,2003,96(3-4):193-205.
[7] Melter O,Radojevic B.Small colony variants of Staphylococcus aureus——Review[J].Folia Microbiol (Praha), 2010,55(6):548-558.
[8] Smith K M,Slugoski M D,Loewen S K,et al.The broadly selective human Na⁺/nucleoside cotransporter (hCNT3) exhibits novel cation-coupled nucleoside transport characteristics[J].J Biol Chem,2005,280(27):25436-25449.
[9] Kahl B,Herrmann M,Everding A S,et al.Persistent infection with small colony variant strains of Staphylococcus aureus in patients with cystic fibrosis[J].J Infect Dis,1998,177(4):1023-1029.
[10] Kahl B C,Duebbers A,Lubritz G,et al.Population dynamics of persistent Staphylococcus aureus isolated from the airways of cystic fibrosis patients during a 6-year prospective study[J]. Journal of Clinical Microbiology,2003,41(9):4424-4427.
[11] Hamill R J,Vann J M,Proctor R A.Phagocytosis of Staphylococcus aureus by cultured bovine aortic endothelial cells:Model for postadherence events in endovascular infections[J].Infect Immun,1986,54(3):833-836.
[12] 刘修权,曲伟杰,高健,等.金黄色葡萄球菌小菌落突变株研究进展[J].中国兽医杂志,2011,47(3):54-57.

[1]	李芷珊, 周照月, 刘天龙, 王帅玉, 江海洋. 基于16S rRNA技术分析桔梗超微粉对小鼠肠道菌群多样性的影响[J]. 中国畜牧兽医, 2025, 52(7): 3372-3385.
[2]	谢欣欣, 唐梓宁, 梁孙圳, 张磊, 李少旭, 刘业君, 唐烨, 王水莲. 益生菌发酵复方蒲公英散的制备及安全性评价[J]. 中国畜牧兽医, 2025, 52(7): 3420-3428.
[3]	周博武, 赵雯, 张业怀, 顾祖华, 杨楷, 熊飙, 居融程, 邓根福, 黄文飞, 胡庭俊, 俸祥仁. 辣蓼散的急性毒性和亚慢性毒性研究[J]. 中国畜牧兽医, 2025, 52(6): 2915-2924.
[4]	曹畅, 李玉莲, 王洁, 何情, 龚艳梅, 范志勇. 外源添加猪胆酸对高脂孕鼠体脂代谢、肠道微生物和胆汁酸代谢的影响[J]. 中国畜牧兽医, 2025, 52(5): 1999-2011.
[5]	赵娜, 王立中, 朱莲英, 蔡玉梅, 李爽, 李成会. 栗苞水提物对小鼠生长性能、脏器系数、抗氧化功能及组织结构的影响[J]. 中国畜牧兽医, 2025, 52(5): 2056-2065.
[6]	程玉洁, 刘国伟, 陆恩庆, 朱敏. 白皮杉醇对呕吐毒素致小鼠肝损伤的保护作用研究[J]. 中国畜牧兽医, 2025, 52(3): 1447-1454.
[7]	常欣欣, 黄鑫, 范港, 杨佳欣, 张伟伟, 杨清竹, 田哲. 大麻二酚对脂多糖诱导的小鼠乳腺上皮细胞炎症反应和凋亡的抑制作用[J]. 中国畜牧兽医, 2025, 52(1): 60-69.
[8]	杨文哲, 刘克祥, 王诗睿, 赵彤, 潘飞龙, 赵树臣, 赵立佳. 基于线粒体损伤探讨双酚A诱导小鼠睾丸间质细胞凋亡的研究[J]. 中国畜牧兽医, 2024, 51(9): 3739-3751.
[9]	方人来, 陈敬宜, 李杭林, 刘晴, 陈奇, 胡庭俊. 苍朴乌梅散对脂多糖致腹泻小鼠的保护作用[J]. 中国畜牧兽医, 2024, 51(9): 4143-4152.
[10]	何家宇, 杨茗淇, 伍娇, 杨宇, 姚欢, 易金娥, 孔丽. C57BL/6小鼠炎症性肠病模型的建立与指标评价[J]. 中国畜牧兽医, 2024, 51(9): 4153-4163.
[11]	胡明霞, 邓艳萍, 何永芸, 姚玥, 莫晓丹, 黄安, 杨秀芬. 桂郁金醇提物对脂多糖致小鼠肺损伤的保护作用研究[J]. 中国畜牧兽医, 2024, 51(8): 3652-3661.
[12]	吉虹锦, 冶倩楠, 李文龙, 李志鹏, 冯霞, 米思远, 史源钧, 张金柠, 郭健, 俞英. 基于高叶酸小鼠睾丸转录组筛选牛繁殖力关键同源基因[J]. 中国畜牧兽医, 2024, 51(7): 2751-2765.
[13]	王顺博, 王浩, 梁韫怡, 王雯竞, 李紫聪, 徐铮. 怀孕后期饲喂利司培酮对母鼠乳腺发育及其幼鼠生长的影响[J]. 中国畜牧兽医, 2024, 51(6): 2489-2500.
[14]	高庆羽, 符乐, 石玉祥, 李杰峰, 刘嫣然, 秦立佳, 张华莹, 何团拥, 王晓芳. 中药组方对大肠杆菌致小鼠腹泻治疗效果研究[J]. 中国畜牧兽医, 2024, 51(6): 2661-2670.
[15]	董媛, 王嘉宁, 刘佳琪, 马广辉, 朱洁, 于欢, 王会岩. 复方中药制剂对H22荷瘤小鼠免疫功能的影响[J]. 中国畜牧兽医, 2024, 51(6): 2717-2725.

金黄色葡萄球菌小菌落突变株感染小鼠乳房炎模型的建立

Estabishment of Mastitis Model in Mouse by Artificial Infection of Staphylococcus aureus Small Colony Variants

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价