梅山猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析

doi:10.16431/j.cnki.1671-7236.2015.12.025

›› 2015, Vol. 42 ›› Issue (12): 3282-3287.doi: 10.16431/j.cnki.1671-7236.2015.12.025

梅山猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析

戴超辉¹, 戴开宇¹, 宗秋芳¹, 包文斌^1,2, 吴圣龙^1,2

1. 扬州大学动物科学与技术学院, 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009;
2. 江苏省种猪繁育和健康养殖工程技术研究中心, 扬州 225009

收稿日期:2015-05-08 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 吴圣龙 E-mail:slwu@yzu.edu.cn
作者简介:戴超辉(1993-),女,湖南新化人,硕士生,研究方向:猪抗病育种,E-mail:chdai1993@163.com
基金资助:
江苏省科技支撑计划(BE2013345、BE2014357);扬州大学大学生科技创新基金项目(201511117030Z)

Polymorphism of Mx1 Gene Exon 14 and its Relationship with Reproductive Performance in Meishan Pigs

DAI Chao-hui¹, DAI Kai-yu¹, ZONG Qiu-fang¹, BAO Wen-bin^1,2, WU Sheng-long^1,2

1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Engineering Research Center for the Reproduction and Healthy Breeding of Boar, Yangzhou 225009, China

Received:2015-05-08 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 为了探讨梅山猪Mx1基因第14外显子的多态性及其对梅山猪繁殖性能的影响,本研究采用PCR-RFLP方法对所采个体Mx1基因第14外显子进行DNA扩增并检测其多态性,同时利用广义线性模型(GLM)分析不同基因型与繁殖性能间的关系。PCR-RFLP结果表明,梅山猪Mx1基因第14外显子Hin6Ⅰ酶切位点存在G36T突变,共检测到2种等位基因,分别定义为:A、B,其中B基因为优势等位基因;共检测到3种基因型,分别定义为:AA、AB、BB,其中BB基因型为优势基因型;关联分析结果表明,在初产母猪中,总产仔数(TNB)、产活仔数(NBA)、断奶仔猪数(LBW)、初生重(BW)及断奶重(WW)这5个繁殖性状在Mx1基因的3种基因型中的差异均存在BB >AB >AA的趋势,BB基因型为有利基因型;且在总产仔数、产活仔数、断奶仔猪数和断奶重中表现出显著差异性(P<0.05);但在经产母猪中均没有显著差异(P>0.05)。本研究结果提示,Mx1基因第14外显子多态性对梅山猪初产母猪的繁殖性能有显著的遗传效应,而对经产母猪的繁殖性能没有显著的影响。

关键词: 梅山猪; Mx1基因; 多态性; 繁殖性能

Abstract: To analyze the effect of different genotypes in Mx1 gene exon 14 on the reproductive performance in Meishan pigs,PCR-RFLP method was used to amplify DNA and detect the polymorphisms of Mx1 gene exon 14, and the relationship between different genotypes and the reproductive performance was further analyzed by generalized linear model (GLM) in this study.The results of PCR-RFLP showed that there was a G36T mutation on Hin6 Ⅰ restriction site in Mx1 gene exon 14 and three genotypes were detected named as AA,AB and BB,in which BB was a dominant genotype.The results of correlation analysis showed that the reproductive performance of three genotypes in Mx1 gene presented the increasing tendency of BB >AB >AA in total number born (TNB),number born alive (NBA),little size at weaning (LBW),birth weight (BW) and weaning weight (WW) in gilts sows on the whole.Moreover,there was significant difference in TNB,NBA,LBW and WW in gilts sows (P< 0.05).However,there was no significant difference among all reproductive performance in multiparous sows (P >0.05).The results of this study indicated that the polymorphisms of Mx1 gene exon 14 had significantly genetic effects on production performance in Meishan gilts sows instead of multiparous sows.

Key words: Meishan pigs; Mx1 gene; polymorphisms; reproductive performance

中图分类号:

S813.3

戴超辉, 戴开宇, 宗秋芳, 包文斌, 吴圣龙. 梅山猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析[J]. , 2015, 42(12): 3282-3287.

DAI Chao-hui, DAI Kai-yu, ZONG Qiu-fang, BAO Wen-bin, WU Sheng-long. Polymorphism of Mx1 Gene Exon 14 and its Relationship with Reproductive Performance in Meishan Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(12): 3282-3287.

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梅山猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析

Polymorphism of Mx1 Gene Exon 14 and its Relationship with Reproductive Performance in Meishan Pigs

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