›› 2009, Vol. 36 ›› Issue (8): 91-93.

• 遗传繁育 • 上一篇    下一篇

牛活体采卵体外受精不分裂MⅡ期卵母细胞的研究

李松，赵祥杰，李华，任兆瑞   

  1. （上海交通大学医学院医学遗传研究所，卫生部医学胚胎分子生物学重点实验室暨上海市生殖与胚胎工程重点实验室， 上海 200040）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-08-20 发布日期:2009-08-20

Research on the MⅡ Stage Bovine Oocytes after IVF Failure

LI Song，ZHAO Xiang-jie，LI Hua，REN Zhao-rui   

  1. （Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, the Key Lab of Medical Embryo Molecular Biology, the Ministry of Health and Shanghai Key Lab of Embryo & Reproductive Engineering, Shanghai 200040, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-08-20 Published:2009-08-20

摘要： 牛活体采卵体外成熟后，常规进行体外受精，平均卵裂率为73.1%。在卵裂率稳定的前提下，随机挑选96枚没有卵裂的MⅡ期卵母细胞进行Hoechst33342染色分析，以期找出卵子没有分裂的原因，为进一步提高卵裂率提供科学资料。结果发现精子没有进入卵母细胞的占59.4%，精子进入卵母细胞但雌雄原核未融合的占19.8%，多精受精引起发育阻滞的占13.5%。结果表明，在保证卵母细胞成熟的前提下，引起牛体外受精失败的主要原因是精子没有进入卵母细胞。

关键词: 牛; 体外受精; Hoechst33342染色

Abstract: Bovine oocytes obtained from OPU (ovum pick up ) were subjected to IVF (in vitro fertilization) and 96 oocytes at MⅡ stage but with IVF failure were selected to stain with Hoechst33342. The data showed that 57 oocytes (59.4%) were not observed the presence of male pronuclei, and the proportion which female and male nuclei did not fuse was 19.8% (19/96), while multi-sperm entrance accounted for 13.5% (13/96). The results suggested that the main reason of bovine IVF failure, on the precondition of oocyte maturation, was caused by the failure of sperm entrance. 

Key words: bovine; IVF; Hoechst33342 stain

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