关键词: 孤雌激活; 大鼠; 卵母细胞; 6-DMAP

Abstract: In this study, oocytes were activated by ethanol, electricity stimulation, or were activated by ethanol combined with the 6-DMAP and electricity stimulation combined with the 6-DMAP to deternine optimal conditions of parthenogenetic activation of Wistar rat oocyts. The results are as follows：
Although electricity stimulation, ethanol and SrCl2+ along were able to activate Wistar rat oocytes, the rates of oocyte activation were relatively low and none of the activated oocytes developed beyond 2cell stage after in vitro culture.For the oocytes were activated by electronic stimulation alone in the same electronic filed, the percentage of activated oocytes were higher when two pulses treatment were given than when one pulse was given. And the highest percentage was achieved when oocyes were activated at the electronic field of 100 V/mm.The percentages of activated oocytes with pronuclear and blastocyst development were significantly higher after treatment with ethanol or electrical stimulation in combined with 6-DMAP than that of ethanol or electrical stimulation alone.

Key words: parthenogenetic activation; rat; oocyte; 6-DMAP