两种转移因子对犬外周血T细胞亚群的影响

›› 2007, Vol. 34 ›› Issue (7): 56-59.

两种转移因子对犬外周血T细胞亚群的影响

孔庆波1,3，陈进军2，陈德坤3，张彦明3

1.中国刑事警察学院警犬技术系，沈阳110034；2.广东海洋大学农业生物技术研究所，湛江524088；3.西北农林科技大学动物科技学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-20 发布日期:2007-07-20

Effects of Two Transfer Factors on T-Lymphocytic Subgroups in Canine Peripheral

KONG Qingbo1,3, CHEN Jinjun2, CHEN Dekun3, ZHANG Yanming3

1.Dept of Police Dog Technology, Criminal Police College of China, Shenyang 110034, China;2. Institute of Agricultural Biotechnology, Guangdong Ocean University, Zhanjiang 524088, China;3. College of Animal Science and Veterinary Medicine, Northwest A＆F University, Yangling 712100, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20

摘要/Abstract

摘要： 按标准方法分别提取制备了猪和犬的转移因子，选择杂种牧羊犬为试验犬，用流式细胞术检测了试验犬注射猪、犬转移因子前和注射后不同时间T细胞亚群的变化情况。结果表明，同种、异种转移因子均可提高试验犬外周血淋巴细胞数量；CD4/CD8比值变化分析结果证明，外周血增加的淋巴细胞主要为CD4+ T细胞；试验犬注射同种、异种转移因子后20 d内，CD4+ T细胞持续增加；同种转移因子促进试验犬外周血CD4+ T细胞上升的作用强于异种转移因子。

关键词: 转移因子; 流式细胞术; 犬外周血; T细胞亚群

Abstract: The requisite swine transfer factor (TF) and canine TF were prepared by relative standards, respectively and were muscularly injected to a range of hybrid shepherd dogs to determine the changes of T-lymphocytic subgroups in different time by flow cytometry. The results were as follows：With regard to enhancement of the number of lymphocyte in canine peripheral blood of homogeneous TF or heterogeneous TF, the increased were mainly CD4+ Tcells, according to the analysis of the ratio of CD4/CD8. CD4+ T-cells had increased for 20 days since the employed dogs were injected with homogeneous TF or heterogeneous TF, respectively.And the enhancive effect of homogeneous TF was better than that of heterogeneous TF.

Key words: transfer factor; flow cytometry; canine peripheral blood; T-lymphocytic subgroup

中图分类号:

S829.2

孔庆波;陈进军;陈德坤;张彦明 . 两种转移因子对犬外周血T细胞亚群的影响 [J]. , 2007, 34(7): 56-59.

KONG Qingbo;CHEN Jinjun;CHEN Dekun;ZHANG Yanming. Effects of Two Transfer Factors on T-Lymphocytic Subgroups in Canine Peripheral[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(7): 56-59.

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