马乳酒样乳杆菌JK-24对DSS诱导的小鼠结肠炎的缓解作用

doi:10.16431/j.cnki.1671-7236.2025.09.036

摘要/Abstract

摘要： 【目的】探究马乳酒样乳杆菌(Lactobacillus kefiranofaciens)JK-24对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎的缓解作用。【方法】将18只C57BL/6小鼠随机分为3组，分别为空白对照组(Control)、模型组(DSS)、马乳酒样乳杆菌JK-24组(JK-24＋DSS)，每组6只。试验第1天起，JK-24＋DSS组小鼠按0.1 mL/10 g灌胃马乳酒样乳杆菌JK-24菌悬液(浓度为10⁹ CFU/mL)，Control和DSS组小鼠灌胃等量生理盐水，连续灌胃21 d；第15～21天DSS和JK-24＋DSS组小鼠自由饮用3% DSS溶液，Control组小鼠正常饮水。试验期间记录小鼠体重变化，观察小鼠粪便情况，统计小鼠疾病活动指数(DAI)。试验结束后采集小鼠结肠和血液样品，测量结肠长度并观察结肠组织病理学变化，测定血清和结肠组织中氧化应激指标和炎症细胞因子水平。【结果】与Control组相比，DSS组小鼠体重明显下降，粪便不成型甚至出现血便，DAI评分显著升高(P＜0.05)，结肠组织可见大量炎性细胞，肠黏膜结构被破坏；血清和结肠组织中炎性因子白细胞介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)、IL-10和IL-6含量显著或极显著升高(P＜0.05；P＜0.01)，超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性极显著下降(P＜0.01)，髓过氧化物酶(MPO)活性和丙二醛(MDA)含量极显著升高(P＜0.01)。与DSS组相比，JK-24＋DSS组小鼠体重下降相对缓慢，DAI评分在造模后期下降，结肠长度显著增加(P＜0.05)，结肠组织炎性细胞浸润区域明显减少，结肠黏膜组织结构基本完整，仅部分区域出现腺体缺失和排列不整齐；血清和结肠组织中IL-6、IL-10、IL-12和TNF-α含量均显著或极显著降低(P＜0.05；P＜0.01)，血清中SOD和CAT活性均极显著升高(P＜0.01)，MPO活性显著下降(P＜0.05)，MDA含量极显著下降(P＜0.01)。【结论】马乳酒样乳杆菌JK-24对DSS诱导的小鼠结肠炎有显著的缓解作用，主要作用机制与其提高机体的抗氧化功能及减少炎性因子的产生有关。

关键词: 马乳酒样乳杆菌; 葡聚糖硫酸钠(DSS); 结肠炎; 抗氧化作用

Abstract: 【Objective】 The purpose of this experiment was to investigate the alleviation effect of Lactobacillus kefiranofaciens JK-24 on dextran sodium sulfate (DSS)-induced colitis in mice. 【Method】 18 C57BL/6 mice were randomly divided into 3 groups:Blank control (Control),model (DSS) and Lactobacillus kefiranofaciens JK-24 (JK-24＋DSS) groups，and 6 mice in each group.From the first day of the experiment,mice in JK-24＋DSS group were gavaged with Lactobacillus liquidacidum JK-24 suspension at 0.1 mL/10 g (with a concentration of 10⁹ CFU/mL).Mice in Control and DSS groups were gavaged with the same amount of normal saline for 21 consecutive days.From the 15th to the 21st day,mice in DSS and JK-24＋DSS groups freely drank 3% DSS solution,while mice in Control group drank water normally.During the experiment,the weight changes of mice were recorded,the fecal conditions were observed,and the disease activity index (DAI) was calculated.After the experiment,colon and blood samples of mice were collected.The length of the colon was measured and the histopathological changes of the colon tissue were observed.The levels of oxidative stress indicators and inflammatory cytokines in serum and colon tissue were determined. 【Result】 Compared with Control group,the weight of mice in DSS group decreased significantly,and the feces did not form or even showed bloody stools,DAI score increased significantly (P＜0.05).A large number of inflammatory cells were seen in colon tissues,the intestinal mucosal structure was destroyed.The contents of inflammatory factors interleukin-12 (IL-12),tumor necrosis factor-α (TNF-α),IL-10 and IL-6 in serum and colonic tissues were significantly or extremely significantly increased (P＜0.05 or P＜0.01).The activities of superoxide dismutase (SOD) and catalase (CAT) decreased extremely significantly (P＜ 0.01),while the activity of myeloperoxidase (MPO) and the content of malondialdehyde (MDA) increased extremely significantly (P＜0.01).Compared with DSS group,the body weight of mice in JK-24＋DSS group decreased relatively slowly,and the DAI score decreased in the later stage of modeling.The length of colon increased significantly (P＜0.05),the area of inflammatory cell infiltration in colonic tissue decreased significantly,and the structure of colonic mucosal tissue was basically intact.Only in some areas did glandular absence and irregular arrangement occur.The contents of IL-6,IL-10,IL-12 and TNF-α in serum and colonic tissue were significantly or extremely significantly decreased (P＜0.05 or P＜0.01).The activities of SOD and CAT in serum were extremely significantly increased (P＜0.01),the activity of MPO was significantly decreased (P＜0.05),and the content of MDA was extremely significantly decreased (P＜0.01). 【Conclusion】 Lactobacillus kefiranofaciens JK-24 had a significant alleviating effect on DSS-induced colitis in mice.The main mechanism of action was related to its improvement of the antioxidant function and reduction of the production of inflammatory factors.

Key words: Lactobacillus kefiranofaciens; dextran sodium sulfate(DSS); colitis; antioxidant effect

中图分类号:

S816.79

张华, 许志芹, 周闯, 乔怡然, 胡粲然, 林家琪, 荀佳琦. 马乳酒样乳杆菌JK-24对DSS诱导的小鼠结肠炎的缓解作用[J]. 中国畜牧兽医, 2025, 52(9): 4406-4415.

ZHANG Hua, XU Zhiqin, ZHOU Chuang, QIAO Yiran, HU Canran, LIN Jiaqi, XUN Jiaqi. Alleviation Effect of Lactobacillus kefiranofaciens JK-24 on DSS-induced Colitis in Mice[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4406-4415.

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