莱鲍迪甙A对雌性小鼠生殖系统及繁殖性能的影响

doi:10.16431/j.cnki.1671-7236.2025.09.015

摘要/Abstract

摘要： 【目的】探究天然无热量甜味剂莱鲍迪甙A(rebaudioside A，Reb A)对雌性小鼠生殖系统及繁殖性能的影响，为生产中进一步应用Reb A提供参考。【方法】选取24只健康雌性ICR小鼠(29.11 g±1.21 g)，分为对照组和试验组(Reb A)，每组3个重复，每个重复4只。对照组小鼠连续14 d灌胃200 mg/(kg·d)生理盐水，Reb A组小鼠连续14 d灌胃200 mg/(kg·d) Reb A，记录每日体重、采食量、饮水量及后代窝产仔数、后代体重。另选取24只健康雌性ICR小鼠(28.88 g±0.93 g)，分组及处理方法同前，通过阴道涂片结合外阴观察法监测小鼠发情周期，采取酶联免疫法(ELISA)检测血清中雌二醇、促卵泡生成激素、促黄体生成素和孕酮水平，利用HE染色检测卵巢组织的组织形态，卵泡种类和数量，超数排卵和体外受精后检测排卵数量、卵裂率和囊胚率，采用荧光染色法检测卵母细胞中活性氧(reactive oxygen species，ROS)及谷胱甘肽(glutathione，GSH)水平，对子宫和卵巢进行称重，并计算其脏器系数。【结果】与对照组相比，灌胃14 d Reb A不会改变雌性小鼠的体重、采食量、饮水量，但能显著提高窝产仔数及后代体重(P＜0.05)；同时，灌胃14 d Reb A能够显著降低其异常发情的比例(P＜0.05)，但对血清中雌二醇、促卵泡生成激素、促黄体生成素和孕酮水平均无显著影响(P＞0.05)。此外，灌胃14 d Reb A可显著增加小鼠子宫重量及卵巢中腔前卵泡数量(P＜0.05)，显著提高体外受精的卵裂率和囊胚率(P＜0.05)，显著降低ROS水平(P＜0.05)，并能够极显著提升卵母细胞GSH水平(P＜0.01)。【结论】本试验条件下，灌胃200 mg/(kg·d) Reb A不会对小鼠的生长性能造成负面影响，同时能够改善小鼠卵母细胞质量，提高胚胎发育能力及繁殖能力。

关键词: 莱鲍迪甙A(Reb A); 小鼠; 繁殖性能; 胚胎发育

Abstract: 【Objective】 This study was aimed to investigate the effects of the natural non-caloric sweetener rebaudioside A (Reb A) on the reproductive system and reproductive performance in female mice,so as to provide a reference for its further application in production. 【Method】 Twenty-four healthy female ICR mice (29.11 g±1.21 g) were divided into control and Reb A groups,with 3 replicates per group and 4 mice per replicate.Mice in control group received 200 mg/(kg·d) saline via oral gavage daily for 14 days,while mice in Reb A group received 200 mg/(kg·d) Reb A.Daily body weight,feed intake,water consumption,litter size,and offspring weight were recorded.Another 24 healthy female ICR mice (28.88 g±0.93 g) were grouped and treated similarly.The estrous cycle was monitored via vaginal smear combined with vulvar observation.The levels of estradiol,follicle-stimulating hormone (FSH),luteinizing hormone (LH),and progesterone in serum were measured by enzyme-linked immunosorbent assay (ELISA).Ovarian histomorphology,follicular types and counts were assessed using hematoxylin and eosin (HE) staining.Superovulation and in vitro fertilization were performed to evaluate ovulation count,cleavage rate,and blastocyst rate.Reactive oxygen species (ROS) and glutathione (GSH) levels in oocytes were measured by fluorescence staining.Uterine and ovarian weights were recorded,and organ coefficients were calculated. 【Result】 Compared with control group,14-day Reb A administration did not alter body weight,feed intake,or water consumption in female mice,but significantly increased litter size and offspring weight (P＜0.05).Reb A also significantly reduced the proportion of abnormal estrus (P＜0.05),however,there was no significant effect on the levels of estradiol,follicle-stimulating hormone,luteinizing hormone and progesterone in serum(P＞0.05).Additionally,Reb A could significantly increase the uterine weight and preantral follicle count in ovaries (P＜0.05),significantly elevate cleavage and blastocyst rates during in vitro fertilization (P＜0.05),significantly reduce ROS level in oocytes (P＜0.05),and extremely significantly enhance GSH level (P＜0.01). 【Conclusion】 Under the experimental conditions,oral administration of 200 mg/(kg·d) Reb A did not negatively affect growth performance in mice,but improve oocyte quality,embryonic developmental potential,and reproductive capacity.

Key words: rebaudioside A (Reb A); mice; reproductive performance; embryonic development

中图分类号:

S814

汝庆妍, 王蕊彬, 陈贵凤, 赵梦琪, 雷安民. 莱鲍迪甙A对雌性小鼠生殖系统及繁殖性能的影响[J]. 中国畜牧兽医, 2025, 52(9): 4163-4172.

RU Qingyan, WANG Ruibin, CHEN Guifeng, ZHAO Mengqi, LEI Anmin. Effects of Rebaudioside A on the Reproductive System and Reproductive Performance in Female Mice[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4163-4172.

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