1株导致种鸭产蛋下降的新型鸭环状病毒分离与鉴定

doi:10.16431/j.cnki.1671-7236.2026.01.035

Abstract

Abstract:

Objective Since August 2021， breeding duck flocks in Anhui， Shandong and other regions had experienced unexplained decreases in egg production， accompanied by symptoms such as depression and ovarian hemorrhage. This study aimed to clarify the pathogenic characteristics of the causative agent and provide a basis for the prevention and control of decreased egg production in breeding ducks. Method Virus isolation was performed using duck embryo fibroblasts （DEF） on ovarian tissue samples from diseased ducks collected in Yishui， Shandong， in September 2022. Next-generation sequencing technology was used to sequence the viral genome， which was compared with the reference virus genome database to determine the pathogen species. The viral genome structure was analyzed， and the encoded proteins were clarified. Specific primers were designed based on the sequenced viral sequences for pathogen identification. The viral genes were compared with reference sequences for similarity and phylogenetic analysis to explore the genetic characteristics and evolutionary relationships of the virus. Regression experiments were carried out using breeding ducks at the peak of egg production to verify the pathogenicity of the virus. Result After inoculating the diseased tissue samples into DEF cell， the cells showed typical cytopathic effects. Whole-virome comparison showed that there were virus sequences closely related to Orbiviridae， and after sequence assembly， it was designated as Duck orbivirus （DORV）. The genome consisted of 10 double-stranded RNA segments， encoding 12 proteins. PCR results confirmed that the pathogen in this study was DORV， and the isolate strain was named DORV-SDYS. The DORV-SDYS strain was closely related to Parry Lagoon virus （PLV）， Corriparta virus （CORV） and Acado virus （ACAV）. The nucleotide sequence similarity of the VP5 fragment was 80.1%-82.4%， and the amino acid sequence similarity was 88.1%-91.1%. Phylogenetic analysis showed that DORV-SDYS had distinct evolutionary characteristics. Animal regression experiments confirmed that DORV-SDYS could lead to a decrease in the egg production rate of breeding ducks， and caused pathological damage to ovarian follicles. Conclusion In this study， a virus was successfully isolated from the ovarian tissue of laying ducks with decreased egg production in Yishui， Shandong province， and it was designated as DORV. The characteristics of the full coding region of its genome were clarified. It was confirmed that DORV infection was the etiological factor causing decreased egg production in breeding ducks， filled the gap in the research on the pathogenicity of Orbivirus in waterfowl hosts， and was of great significance to the healthy development of the waterfowl industry.

Key words: Duck orbivirus; breeding duck; mosquito-borne virus; laying performance

CLC Number:

S858.3

LI Bing, XU Mingqing, HAO Dongmin, WANG You, LI Huihui, MAO Mingtian, WU Mian, LU Chengguang, TANG Jing, LIANG Suyun, GUO Zhanbao, ZHOU Zhengkui, HOU Shuisheng, TANG Yi. Isolation and Identification of a Novel Duck Orbivirus Causing Decreased Egg Production in Breeding Ducks[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(1): 390-401.

Figures/Tables 15

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References 32

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病毒 Viruses	引物序列 Primer sequences （5′→3′）	长度 Length/bp	登录号 Accession No.
鸭环状病毒 DORV	F：GATACTGGAAGAGATGCTACA	565	PV871612
鸭环状病毒 DORV	R：CTGACGCTTGAGACACATA	565	PV871612
禽流感病毒 AIV	F：GACCRATCCTGTCACCTCTGA	346	PV950474.1、 PV949762.1
禽流感病毒 AIV	R：TGTCTGTGGGACCGATGCTGT	346	PV950474.1、 PV949762.1
鸭瘟病毒 DPV	F：GAAGGCGGGTATGTAATGTACA	447	OP502744.1
鸭瘟病毒 DPV	R：TCAAGGCTCTATTCGGTAATGATC	447	OP502744.1
鸭坦布苏病毒 TMUV	F：AAGCGAGCACCTACCATA	539	MK276456.1
	R：CAATTCCACCACGACAGT	539	MK276456.1

病毒 Virus	毒株 Strains	登录号 Accession No.	病毒 Virus	毒株 Strains	登录号 Accession No.
鸭环状病毒 DORV	SDYS	PV871612	帕里泻湖病毒 PLV	K71551	KU724114.1
非洲马瘟病毒 AHSV	RSA	ON809528.1	尤马蒂拉病毒 UMAV	IA08	PP669540.1
	2/E	KT030364.1		DE	MK100591.1
	7/E	KT030564.1		USA1969	HQ842624.1
蓝舌病病毒 BTV	BN96	JN671911.1	阿卡多病毒 ACAV	EthAr 1846-64	OL709425.1
	BTV17	MT952976.1	科里帕塔病毒 CORV	MP416-NA-2018	MW809644.1
	France	OR500249.1	科里帕塔病毒 CORV	AUS1960	KC853047.1
昌古尼拉病毒 CGNV	202 A	KY764793.1	中点环状病毒 MPOV	10088	MZ079850.1
	GBT 1144	KY659449.1		V9619	MZ079840.1
	D 50	KY659439.1		V9550	MZ079830.1
乔巴尔峡谷病毒 CGV	NEP1970	KP268789.1	云南环状病毒 YUOV	ON-4	LC585877.1
CHeRI环状病毒 CHeRIV	OV895	MT341506.1		JKT-10087	MF152992.1
CHeRI环状病毒 CHeRIV	OV926	MK903673.1		OV1288	MW424405.1

病毒 Virus	登录号 Accession No.	得分 Score
帕里泻湖病毒 PLV	KU724110.1	2 534
科里帕塔病毒 CORV	KC853046.1	1 219
科里帕塔病毒 CORV	NC_038566.1	1 219
阿卡多病毒 ACAV	OL709420.1	870
帕里泻湖病毒 PLV	KU724113.1	2 477
科里帕塔病毒 CORV	KC853047.1	1 214
科里帕塔病毒CORV	NC_038571.1	1 214
帕里泻湖病毒 PLV	KU724114.1	1 945

节段 Segments	编码蛋白 Encoded protein	蛋白功能 Protein function	开放阅读框长度 ORF length/bp	氨基酸长度 Amino acid length	蛋白质分子质量 Protein molecular weight/ku
1	VP1	RNA聚合酶	3 873	1 290	147.1
2	VP2	核心蛋白	2 850	949	108.5
3	VP3	外层衣壳蛋白	2 229	742	84.6
4	VP4	加帽酶	1 929	642	75.0
5	NS1	管状蛋白	1 779	592	69.2
6	VP5	外层衣壳蛋白	1 584	527	57.8
7	NS2	病毒包涵体蛋白	1 116	371	41.7
8	VP7	核心蛋白	1 068	355	39.3
9	VP6	螺旋酶	1 035	344	36.4
9	NS4	非结构蛋白	459	152	18.5
10	NS3	非结构蛋白	717	238	26.1
10	NS3a	非结构蛋白	318	105	12.3

Isolation and Identification of a Novel Duck Orbivirus Causing Decreased Egg Production in Breeding Ducks

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