野生黑鸢源芽孢杆菌的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2025.11.043

Abstract

Abstract: 【Objective】 This study aimed to isolate and identify Bacillus strains with lower antibiotic resistance from the feces of wild Milvus migrans,and determine the potential beneficial effects,so as to provide candidate strains for animal nutrition and feed microecological preparations. 【Method】 Fresh faecal samples of wild Milvus migrans were collected from Xinjiang wildlife rescue station,and Bacillus was isolated with LB agar medium.Morphological observation,biochemical identification and 16S rDNA sequencing were performed.The ability of acid and bile salt resistance,self-aggregation,DPPH free radical clearance,drug sensitivity,hemolytic activity and antibacterial ability of the isolated strains were further determined to evaluate their in vitro probiotic properties. 【Result】 Three strains of bacteria were isolated from the feces of wild Milvus migrans.On LB agar medium,the isolated strains were light yellow folded single colony with rough and wet surface.They were Gram-positive rod-shaped bacteria,named C42a,C45 and C5y1, respectively.The biochemical identification results showed that the test results of V-P,D-xylose, L-arabinose,D-mannitol,gelatin liquefaction,pH 5.7,7% sodium chloride,nitrate reduction and starch hydrolysis of the three isolated bacteria were positive,and the results of the citrate and propionate tests were negative.16S rDNA sequencing results showed that the similarities between C42a and Bacillus licheniformis PP236929.1,C45 and Bacillus licheniformis MF581456.1,C5y1 and Bacillus licheniformis HQ850703.1 were all ＞97%,and the three isolates were all identified as Bacillus licheniformis.At pH 2.0,the survival rate of the three strains was higher than 32.00% and the highest was 75.30%.It could survive at a bile salt concentration of 0.3%.The self-aggregation capacity was in the range of 52.22% to 88.89%,and the DPPH free radical clearance was in the range of 63.62% to 84.18%.The results of drug sensitivity test showed that C42a,C45 and C5y1 were sensitive to 11 drugs including ampicillin,imipenem,amikacin,gentamicin and so on.C42a was moderately sensitive to erythromycin,C5y1 was moderately sensitive to clindamycin,and C45 was moderately sensitive to erythromycin and clindamycin.The three strains did not have hemolytic activity.C42a and C45 showed good inhibitory effect on Salmonella and Staphylococcus aureus. 【Conclusion】 In this study,three strains of Bacillus licheniformis C42a,C45 and C5y1 with lower antibiotic resistance were isolated from the feces of wild Milvus migrans,which showed good in vitro probiotics and could be used as candidate strains for animal microecological preparations.

Key words: Milvus migrans; Bacillus licheniformis; isolation and identification; biological characteristics

CLC Number:

S831.5

XIE Huairu, PAN Yuhan, LIN Jun, SHI Rui, WANG Xuxiang, DAI Zongmao, JIN Qian, Dilraba DILIXIATI, Ayiqiaolifan SHAHAN, PENG Qimin, XIAO Ying, MAI Duo, WANG Jinquan. Isolation,Identification and Biological Characteristics of Bacillus Derived from Wild Milvus migrans[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5488-5498.

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Isolation,Identification and Biological Characteristics of Bacillus Derived from Wild Milvus migrans

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