基于Argonaute蛋白的动物病原核酸检测技术及应用研究进展

doi:10.16431/j.cnki.1671-7236.2025.11.035

Abstract

Abstract: Argonaute (Ago) protein is a highly conserved protein and widely found in organisms.It can specifically recognize and cleave complementary targets by binding short guide single-stranded nucleic acid sequence.Based on specific sequence recognition and transfer cleavage activity characteristics of Ago,it has emerged as a promising candidate for a new generation of nucleic acid molecular diagnostic biosensors.It provides a novel choice for developing of rapid,sensitive,specific and multiplex nucleic acid detection technology.In recent years,it has attracted the attention of researchers especially in the field of animal pathogen detection.A variety of animal pathogen detection methods have been established.It shows a great application prospects.The authors reviewed the structures and biotechnologies of Ago,summarized representative nucleic acid detection platforms mediated by Thermus thermophilus Ago protein (TtAgo) and Pyrococcus furiosus Ago (PfAgo) and their application in animal pathogen detection,prospected the potential strategies to improve the application with the aims of providing new ideas for the development of a new generation of animal pathogen nucleic acid molecular diagnostic biosensors.

Key words: Argonaute protein; nucleic acid detection technology; animal diseases; diagnosis

CLC Number:

FENG Jie, LI Chunxiu, YANG Yang, ZOU Bo, FU Lingling, CAO Qianda. Progress on Animal Pathogenic Nucleic Acid Detection Technology Based on Argonaute Protein and Its Application[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5403-5413.

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Progress on Animal Pathogenic Nucleic Acid Detection Technology Based on Argonaute Protein and Its Application

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