鹅胰岛素样生长因子2基因启动子区序列分析

doi:10.16431/j.cnki.1671-7236.2025.10.002

Abstract

Abstract: 【Objective】 The structure and function of promoters are crucial in gene transcriptional regulation research.This study aimed to analyze the structural characteristics and transcriptional regulatory mechanisms of the insulin-like growth factor 2 (IGF2) gene promoter region in goose,providing a theoretical foundation for understanding the role of IGF2 gene in the growth and development of goose.【Method】 Based on the 2 170 bp sequence of the 5'-flanking region of IGF2 gene (GCA_040182565.1) in goose,the promoter activity region was predicted using tools such as Promoter 2.0,and AnimalTFDB 4.0.The binding sites of transcription factors and regulatory elements were analyzed.The TBtools software was employed to analyze the conservation of the IGF2 gene promoter region in goose through cross-species sequence alignment.Moreover,genomic single nueleotide polymorphism (SNP)genotyping data from Huoyan geese and Zhedong White geese were untlized to compare the genetic variations between both geese breeds.Additionally,the transcriptional expression of IGF2 gene in gonadal axis tissues during the egg-laying period was analyzed for both geese breeds.【Result】 The promoter activity region of IGF2 gene in goose was located 1 020-1932 bp,containing critical elements such as the CAAT-box,GC-box,TATA-box,and E-box,which was associated with cell proliferation and differentiation.The transcription factors Sp1 and Irf3 were identified as potential regulators of gene transcription through binding to these elements.Cross-species sequence alignment revealed a high degree of similarity between the promoter regions of geese and Ferruginous ducks,with a similarity of 92.94%.SNP genotyping analysis showed a significantly higher proportion of CC genotype in Huoyan geese (89%) compared with Zhedong White geese (25%),while the proportions of CT and TT genotypes in Zhedong White geese (both were 38%) were markedly higher than those in Huoyan geese (11%).Furthermore,a significant difference in the expression of IGF2 gene was observed in ovarian tissue between the two goose breeds during the egg-laying period (P＜0.05).【Conclusion】 The promoter region of IGF2 gene in goose was abundant in binding sites for various transcription factors,including Sp1 and Irf3,which played crucial roles in regulating the stable expression of IGF2 gene.Additionally,a SNP variation (C/T) at position 1 450 bp in the promoter region of IGF2 gene had been identified in the germplasms of both Huoyan geese and Zhedong White geese.This genetic variation could affect the growth,development,and egg production performance of geese by influencing the expression of IGF2 gene.

Key words: goose; IGF2 gene; promoter region; transcription factor; CpG island

CLC Number:

S835.2
Q78

ZHAO Hongchang, MU Xiaohui, CHEN Chong, LI Xiaoming, WANG Jun, ZHAO Mengli, YU Jun, XIE Jia, ZHANG Wei, HU Tuanjun, WANG Jian. Sequence Analysis of Insulin-like Growth Factor 2 Gene Promoter Region in Geese[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4538-4548.

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Sequence Analysis of Insulin-like Growth Factor 2 Gene Promoter Region in Geese

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