鸭坦布苏病毒检测方法及疫苗研究进展

doi:10.16431/j.cnki.1671-7236.2023.04.005

Abstract

Abstract: Duck Tembusu virus (DTMUV) is an emerging mosquito-borne virus of the genus Flavivirus in the family Flaviviridae.The disease caused by it is characterized by the decline of egg production of laying ducks and the damage of the nervous system of ducklings in clinical practice,which has caused significant economic losses to the domestic duck industry.Since its outbreak in Shanghai in 2010,DTMUV infection has gradually spread to major duck farms in Eastern,Southeastern,and Northern China.Several clinical diagnostic or laboratory tests for DTMUV have been established in China.Isolation and identification of DTMUV and immunohistochemistry are used to detect the presence of viral particles.Serological detection of DTMUV mainly includes indirect ELISA,competitive ELISA,and colloidal gold immunochromatographic strips,which can detect DTMUV-specific proteins or antibodies and is widely used for the field detection of DTMUV.The detection of DTMUV by conventional PCR,multiplex PCR and CRISPR techniques has enabled rapid clinical diagnosis of DTMUV and facilitated the epidemiological investigation and study of the pathogenesis of duck tambours virus disease.Currently,with the development and commercialization of some vaccines,the epidemic intensity of DTMUV infection in China has gradually changed from outbreaks to sporadic transmission.However,DTMUV has a wide host range,diverse transmission routes,and potential zoonotic potential.To identify and interrupt the spread of DTMUV at an early stage,there is an urgent need to establish rapid,accurate,safe,and effective detection methods and vaccines.The author summarizes the research progress of DTMUV detection methods and vaccines to provide references for the prevention of DTMUV infection.

Key words: Duck Tambusu virus (DTMUV); detection method; vaccine

CLC Number:

S852.65

CHEN Jimin, LI Huihuang, LAI Lianjie, YANG Yinhua, YANG Yue, LIN Ruiyi. Advance on Detection Methods and Vaccines of Duck Tembusu Virus[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(4): 1329-1339.

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Advance on Detection Methods and Vaccines of Duck Tembusu Virus

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