两种有机锌源对新生犊牛生长性能及锌代谢的影响

doi:10.16431/j.cnki.1671-7236.2022.09.012

Abstract

Abstract: 【Objective】 This study aimed to investigate the effects of supplementation with two different organic zinc sources on the growth performance and zinc metabolism of newborn dairy heifers.【Method】 Thirty newborn Holstein heifers with similar body weight (BW 39.4 kg±0.8 kg) were selected and randomly divided into 3 groups, with 10 heifers in each group.Heifers in the control group (CON) was fed basal diet without organic zinc supplementation, and heifers in organic zinc groups were fed with 522.82 mg/d of zinc proteinate (Zn-Pro) or 467.88 mg of zinc methionine (Zn-Met) (equivalent to 80 mg Zn).The experiment lasted for 14 d.Before morning feeding on day 1 and 14, the body weight of each calf was measured to calculate the average daily gain.Milk intake was recorded daily and used to calculate average daily feed intake and F/G.The feces score was assessed by a specialized person before morning feeding every day, and the incidence of diarrhea was calculated.Before morning feeding on day 14, the serum samples were collected for the determination of calcium, phosphorus, zinc, copper and iron concentrations, activities of alkaline phosphatase and superoxide dismutase, metallothionein concentration, malondialdehyde concentration and total antioxidant capacity.【Result】 Compared with the CON group, supplementation with Zn-Met significantly increased the average daily gain of dairy heifers (P<0.05), supplementation with Zn-Pro and Zn-Met decreased the incidence of diarrhea and increased the serum zinc concentration of heifers (P<0.05), and the serum zinc concentration of heifers in Zn-Met group was significantly higher than that in Zn-Pro group (P<0.05).Compared with the CON group, supplementation with Zn-Met significantly decreased the serum copper concentration of heifers (P<0.05), but there was no significant difference between the Zn-Pro group and the control group(P>0.05).Compared with the CON group, supplementation with Zn-Pro and Zn-Met both significantly increased the serum concentration of metallothionein (P<0.05), but only Zn-Met increased serum activity of alkaline phosphatase (P<0.05).Superoxide dismutase activity and total antioxidant capacity in serum of heifers were increased by Zn-Met supplementation (P<0.05), but supplementation with Zn-Pro had no significant effect (P>0.05).Supplementation with Zn-Pro and Zn-Met reduced the concentration of malondialdehyde in serum of dairy heifers (P<0.05).【Conclusion】 Supplementation with Zn-Met in the diet improved the growth performance, reduced the incidence of diarrhea, and improved the zinc metabolism of dairy heifers, whose effects were better than that of Zn-Pro.Therefore, it was suggested to add 80 mg Zn/d as Zn-Met to the diet of dairy heifers.

Key words: zinc proteinate; zinc methionine; newborn heifer; growth performance; zinc metabolism

CLC Number:

S823

WO Yeqianli, GAO Duo, MA Fengtao, JIN Yuhang, LIU Zhuo, SUN Peng. Effects of Two Different Organic Zinc Sources Supplementation on Growth Performance and Zinc Metabolism of Newborn Heifers[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(9): 3382-3390.

References

[1] YASUI G, KATAYAMA S, KUBOTA Y, et al.Zinc finger protein 483(ZNF483) regulates neuronal differentiation and methyl-CpG-binding protein 2(MeCP2) intracellular localization[J].Biochemical and Biophysical Research Communications, 2021, 568:68-75.
[2] SONG Y, LEONARD S W, TRABER M G, et al.Zinc deficiency affects dna damage, oxidative stress, antioxidant defenses, and DNA repair in rats[J].Journal of Nutrition, 2009, 139(9):1626-1631.
[3] NAKAMURA H, SEKIGUCHI A, OGAWA Y, et al.Zinc deficiency exacerbates pressure ulcers by increasing oxidative stress and ATP in the skin[J].Journal of Dermatological Science, 2019, 95(2):62-69.
[4] HU H, XIA N, LIN J, et al.Zinc regulates glucose metabolism of the spinal cord and neurons and promotes functional recovery after spinal cord injury through the ampk signaling pathway[J].Oxidative Medicine and Cellular Longevity, 2021, 2021:4331625.
[5] BARRINGTON G M, PARISH S M.Bovine neonatal immunology[J].Veterinary Clinics of North America:Food Animal Practice, 2001, 17(3):463-476.
[6] ADAB M, MAHJOUBI E, YAZDI M H, et al.Effect of supplemental dietary zinc and its time of inclusion on pre-weaning phase of Holstein heifer calves:Growth performance and health status[J].Livestock Science, 2020, 231:103891.
[7] CHO Y I, YOON K J.An overview of calf diarrhea-infectious etiology, diagnosis, and intervention[J].Journal of Veterinary Science, 2014, 15(1):1-17.
[8] FELDMANN H R, WILLIAMS D R, CHAMPAGNE J D, et al.Effectiveness of zinc supplementation on diarrhea and average daily gain in pre-weaned dairy calves:A double-blind, block-randomized, placebo-controlled clinical trial[J].PLoS One, 2019, 14(7):e0219321.
[9] WEI J Y, MA F T, HAO L Y, et al.Effect of differing amounts of zinc oxide supplementation on the antioxidant status and zinc metabolism in newborn dairy calves[J].Livestock Science, 2019, 230:103819.
[10] ANGELES-HERNANDEZ J C, MIRANDA M, MUNOZ-BENITEZ A L, et al.Zinc supplementation improves growth performance in small ruminants:A systematic review and meta-regression analysis[J].Animal Production Science, 2021, 61(7):621-629.
[11] CEYLAN M N, AKDAS S, YAZIHAN N.Is zinc an important trace element on bone-related diseases and complications? a meta-analysis and systematic review from serum level, dietary intake, and supplementation aspects[J].Biological Trace Element Research, 2021, 199(2):535-549.
[12] OUYANG Z, REN P, ZHENG D E, et al.Hydrothermal synthesis of a new porous zinc oxide and its antimicrobial evaluation in weanling piglets[J].Livestock Science, 2021, 248:104499.
[13] PEI X, XIAO Z, LIU L, et al.Effects of dietary zinc oxide nanoparticles supplementation on growth performance, zinc status, intestinal morphology, microflora population, and immune response in weaned pigs[J].Journal of the Science of Food and Agriculture, 2019, 99(3):1366-1374.
[14] 王晓佳.有机锌在养猪生产中的作用[J].养猪, 2021, 1:18-19. WANG X J.The role of organic zinc in pig production[J].Swine, 2021, 1:18-19.(in Chinese)
[15] AO T, PIERCE J L, POWER R, et al.Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks[J].Poultry Science, 2009, 88(10):2171-2175.
[16] AKSU T, AKSU M, YORUK M A, et al.Effects of organically-complexed minerals on meat quality in chickens[J].British Poultry Science, 2011, 52(5):558-563.
[17] 段静娜.大颗粒晶型蛋氨酸锌络合物制备方法及在蛋鸡预混料中的应用[J].畜牧兽医科学(电子版), 2021, 1:17-18. DUAN J N.Preparation method of large-particle crystalline zinc methionine complex and its application in layer premix[J].Animal Husbandry and Veterinary Science (Electronic Edition), 2021, 1:17-18.(in Chinese)
[18] BHAGWAT V G, BALAMURUGAN E, RANGESH P.Cocktail of chelated minerals and phytogenic feed additives in the poultry industry:A review[J].Veterinary World, 2021, 14(2):364-371.
[19] CHANG M N, WEI J Y, HAO L Y, et al.Effects of different types of zinc supplement on the growth, incidence of diarrhea, immune function, and rectal microbiota of newborn dairy calves[J].Journal of Dairy Science, 2020, 103(7):6100-6113.
[20] LIANG X, CAO C, CHEN P, et al.Effects of dietary zinc sources and levels on growth performance, tissue zinc retention and antioxidant response of juvenile common carp (Cyprinus carpio var.Jian) fed diets containing phytic acid[J].Aquaculture Nutrition, 2020, 26(2):410-421.
[21] ZHU Y, LI F, YU M.Recent progress in application of zinc glycine in animal production[J].Animal Husbandry and Feed Science (Inner Mongolia), 2021, 42(2):52-56.
[22] MA F T, WO Y Q L, SHAN Q, et al.Zinc-methionine acts as an anti-diarrheal agent by protecting the intestinal epithelial barrier in postnatal Holstein dairy calves[J].Animal Feed Science and Technology, 2020, 270:114686.
[23] MA F, WO Y, LI H, et al.Effect of the source of zinc on the tissue accumulation of zinc and jejunal mucosal zinc transporter expression in holstein dairy calves[J].Animals (Basel), 2020, 10(8):1246.
[24] 金宇航, 麻柱, 高铎, 等.不同锌源对新生荷斯坦犊牛生长性能、血清免疫和抗氧化指标以及血浆微量元素含量的影响[J].动物营养学报, 2021, 33(6):3334-3342. JIN Y H, MA Z, GAO D, et al.Effects of different zinc sources on growth performance, serum immune and antioxidant indices and plasma trace element contents of newborn holstein dairy calves[J].Chinese Journal of Animal Nutriton, 2021, 33(6):3334-3342.(in Chinese)
[25] OSORIO J S.Gut health, stress, and immunity in neonatal dairy calves:The host side of host-pathogen interactions[J].Journal Animerica Science Biotechnology, 2020, 11(1):105.
[26] BüCKER R, ZAKRZEWSKI S S, WIEGAND S, et al.Zinc prevents intestinal epithelial barrier dysfunction induced by alpha-hemolysin-producing Escherichia coli 536 infection in porcine colon[J].Veterinary Microbiology, 2020, 243:108632.
[27] BARKLEY J A, PEMPEK J A, BOWMAN A S, et al.Longitudinal health outcomes for enteric pathogens in preweaned calves on Ohio dairy farms[J].Preventive Veterinary Medicine, 2021, 190:105323.
[28] GLOVER A D, PUSCHNER B, ROSSOW H A, et al.A double-blind block randomized clinical trial on the effect of zinc as a treatment for diarrhea in neonatal Holstein calves under natural challenge conditions[J].Preventive Veterinary Medicine, 2013, 112(3-4):338-347.
[29] HAN L, BATISTEL F, MA Y, et al.Methionine supply alters mammary gland antioxidant gene networks via phosphorylation of nuclear factor erythroid 2-like 2(NFE2L2) protein in dairy cows during the periparturient period[J].Journal of Dairy Science, 2018, 101(9):8505-8512.
[30] DENG B, ZHOU X, WU J, et al.Effects of dietary supplementation with tribasic zinc sulfate or zinc sulfate on growth performance, zinc content and expression of zinc transporters in young pigs[J].Animal Science Journal, 2017, 88(10):1556-1560.
[31] KINCAID R L, CHEW B P, CRONRATH J D.Zinc oxide and amino acids as sources of dietary zinc for calves:Effects on uptake and immunity[J].Journal of Dairy Science, 1997, 80(7):1381-1388.
[32] DRESLER S, ILLEK J, ZEMAN L.Effects of organic zinc supplementation in weaned calves[J].Acta Veterinaria Brno, 2016, 85(1):48-53.
[33] HUANG Y L, LU L, LI S F, et al.Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet[J].Journal of Animal Science, 2009, 87(6):2038-2046.
[34] SANTON A, GIANNETTO S, STURNIOLO G C, et al.Interactions between Zn and Cu in LEC rats, an animal model of Wilson's disease[J].Histochemistry Cell Biology, 2002, 117(3):275-281.
[35] MUNKSGAARD N C, BURCHERT S, KAESTLI M, et al.Cadmium uptake and zinc-cadmium antagonism in Australian tropical rock oysters:Potential solutions for oyster aquaculture enterprises[J].Marine Pollution Bulletin, 2017, 123(1-2):47-56.
[36] RADER B A.Alkaline phosphatase, an unconventional immune protein[J].Frontiers in Immunology, 2017, 8:897.
[37] SPEARS J W, KEGLEY E B.Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers[J].Journal of Animal Science, 2002, 80(10):2747-2752.
[38] CHASAPIS C T, NTOUPA P A, SPILIOPOULOU C A, et al.Recent aspects of the effects of zinc on human health[J].Archives of Toxicology, 2020, 94(5):1443-1460.
[39] WRIGHT C L, SPEARS J W.Effect of zinc source and dietary level on zinc metabolism in Holstein calves[J].Journal of Dairy Science, 2004, 87(4):1085-1091.
[40] SKALNY A A, TINKOV A A, MEDVEDEVA Y S, et al.Effect of short-term zinc supplementation on zinc and selenium tissue distribution and serum antioxidant enzymes[J].Journal Acta Scientiarum Polonorum, 2015, 14(3):269-276.
[41] CHEN F, LI Y, SHEN Y, et al.Effects of prepartum zinc-methionine supplementation on feed digestibility, rumen fermentation patterns, immunity status, and passive transfer of immunity in dairy cows[J].Journal of Dairy Science, 2020, 103(10):8976-8985.
[42] GARKHAL J, CHETHAN G E, GUPTA V K, et al.Antioxidant potential of coenzyme Q10 in Escherichia coli associated calf diarrhea[J].Indian Journal of Animal Sciences, 2017, 87(6):694-700.
[43] ALIMOHAMADY R, ALIARABI H, BRUCKMAIER R M, et al.Effect of different sources of supplemental zinc on performance, nutrient digestibility, and antioxidant enzyme activities in lambs[J].Biological Trace Element Research, 2019, 189(1):75-84.
[44] LONG L, CHEN J, ZHANG Y, et al. Comparison of porous and nano zinc oxide for replacing high-dose dietary regular zinc oxide in weaning piglets[J].PLoS One, 2017, 12(8):e0182550.

Effects of Two Different Organic Zinc Sources Supplementation on Growth Performance and Zinc Metabolism of Newborn Heifers

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Jun, YIN Lei, YANG Ying, HU Jianxin, TANG Li, QIN Qingming, LIANG Chengcheng, WU Haigang, ZHAO Mengting, DUAN Wenmiao. Effects of Different Fermentation Bedding Materials on Growth Performance, Digestive Enzyme Activities, Serum Biochemical and Immune Indices in Patridge Shank Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3047-3058.
[2]	LI Duo, YUAN Xinxin, SHEN Zhehong, RUAN Fei, ZHAO Lulu, WANG Chuankun, ZENG Yaqi, YAO Xinkui, MENG Jun. Effects of Proline Supplementation on Growth Performance, Antioxidant and Immune Index and Cytokine Contents of Serum in Weaned Foals [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3084-3092.
[3]	LU Xinxin, FU Kaibo, LI Siman, ZHANG Haihua, ZHANG Haijun, QIU Kai, WU Shugeng. Effect of Dietary Malic Acid Supplemented in the Initial Growth Stage on Growth Performance, Meat Quality, Serum Biochemical and Intestinal Health of Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3104-3115.
[4]	ZHOU Lusong, ZHAO Yuanyuan, YANG Yuwei, ZHAO Qingyu, MA Qing, TANG Chaohua, ZHANG Huiyan, ZHANG Junmin, QIN Yang, QIN Yuchang. Effects of Sophora alopecuroides and Alkaloids from Sophora alopecuroides on Growth Performance and Rumen Microbiota of Tan Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2540-2551.
[5]	LYU Xueze, REN Haiyuan, WANG Liang, XIE Shiyong, YANG Weifang, LIU Jun, WU Shugeng, WU Dimei, LIU Yanhan. Effects of Overfeeding Time on Growth Performance,Slaughter Performance, Nutritional Quality and Intestinal Morphology of Pekin Ducks [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2582-2591.
[6]	LIU Jiayi, WU Hua, SHEN Tong, WANG Kailong, WANG Wensheng, CHEN Zixin. Effects of Extract of Lycium ruthenicum Murr on Growth Performance,Slaughter Performance,Antioxidant Function and Meat Quality of Bamei Ternary Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2637-2649.
[7]	ZHANG Guanfeng, SONG Xianfan, YAN Zhaoming, YANG Xiaojin, ZHENG Mengli, LIU Yating, CHEN Qinghua. Effects of Dietary Superoxide Dismutase Supplementation on Growth Performance, Immune Performance,Antioxidant Capacity and Intestinal Function of Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 1987-1998.
[8]	ZHAO Na, WANG Lizhong, ZHU Lianying, CAI Yumei, LI Shuang, LI Chenghui. Effects of Chestnut Bur Aqueous Extracts on Growth Performance,Organ Coefficient,Antioxidant Function and Tissue Structure in Mice [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2056-2065.
[9]	ZHONG Min, YANG Xiaopei, LIU Ruiping, SONG Wenjing, ZHONG Yunping. Effect of Dietary Andrographis paniculata Extract Supplementation on the Growth Performance,Serum Biochemistries,and Gut Health in Ningdu Yellow Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2115-2127.
[10]	BIAN Zhiyao, LIU Guohua, HAN Shuaijuan, CHEN Zhimin, ZHENG Aijuan. Effect of Dietary Probiotics Supplementation on Growth Performance, Slaughter Performance,Immune Organ Index and Apparent Metabolic Rate of Nutrients in Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2140-2148.
[11]	ZHANG Zhengfei, TANG Anxing, SUN Jindong, FU Lixiang, WANG Xingxian, ZHANG Shiyun, YANG Liangyu, NIU Guoyi, TAO Linli. Study on the Appropriate Dietary Crude Protein Level of Yanjin Black-bone Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1543-1553.
[12]	LAI Qiushuang, LI Lingshuo, LI Shuyi, TIAN Wei, YU Qingting, ZHANG Kanglin, LI Depeng, LIU Jun, JIANG Fei, PENG Zhongli, WU Jianping, HUANG Yanling. Effects of Rumen-protected Methionine Supplementation in Diets with Different Protein Levels on Growth Performance,Rumen Fermentation and Apparent Digestibility of Nutrients in Yaks [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1567-1578.
[13]	OU Jiancun, HUANG Jian, QIU Ting, CEN Mingzhu, ZHENG Chaojun, WANG Wenhe, HUANG Weilong, ZHANG Huihua. Effects of 7 Essential Amino Acids Balanced Low Protein Diet on Growth, Nutrient Digestion and Intestinal Tissue Morphology of Qingyuan Partridge Chickens at 31 to 60 Days of Age [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1102-1112.
[14]	FAN Qiuli, TAO Zhengguo, LI Hui, GOU Zhongyong, WANG Yibing, LIN Xiajing, YE Jinling, JIANG Shouqun. Effects of α-mangostin on Growth Performance,Immune Function, Antioxidant Capacity and Detoxification Function of Broilers [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1123-1130.
[15]	HUANG Xuan, MA Yuyong, LIU Yang, LI Chuang, JIANG Guitao, ZHANG Xu, DENG Ping, WAN Weican, HE Xu, DAI Qiuzhong. Feeding Effects of Granular Alfalfa and Fresh Pasture Partially Replacing Complete Feed on Linwu Ducks [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1159-1165.