乳酸在奶牛体内的代谢途径及其对奶牛健康影响的研究进展

doi:10.16431/j.cnki.1671-7236.2021.07.019

Abstract

Abstract: Lactic acid is an important intermediate metabolite in the rumen of dairy cows.Reasonable regulation of lactic acid metabolic characteristics,full utilization and playing the beneficial functions of lactic acid are of great significance for the healthy production of dairy cows.The synthetic pathway of lactic acid,the main acid-producing bacteria,the metabolic pathway of lactic acid,the main utilizing bacteria,the metabolic mode of lactic acid in the rumen of dairy cattle and the factors affecting its metabolism were reviewed in this paper.The effects of lactic acid-producing bacteria and utilizing bacteria on the regulation of lactic acid metabolism were elaborated in detail.At the same time,the effects of plant extracts on the regulation of lactic acid metabolism and the effects of lactic acid metabolism regulation on the gastrointestinal flora,lactation performance and mastitis of dairy cattle were introduced.It provided a theoretical basis for further understanding the action mechanism of lactic acid on the health of dairy cows and the application of related plant active substances in production practice,and provided new ideas for solving the prevention and treatment of acidosis in dairy cows caused by high-precision diet.

Key words: lactic acid; lactic acid producing bacteria; lactic acid utilizing bacteria; plant extracts; dairy cows

CLC Number:

S823

YU Shiqiang, TONG Jinjin, XIONG Benhai, JIANG Linshu. Research Progress on Metabolic Pathway of Lactic Acid and Its Effect on the Health of Dairy Cows[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(7): 2439-2447.

References

[1] SNYDER E,CREDILLE B.Diagnosis and treatment of clinical rumen acidosis[J].Veterinary Clinics of North America Food Animal Practice,2017,33(3):451-461.
[2] TORU O,HIROKI M,NAOKI I,et al.Long-term high-grain diet altered the ruminal pH,fermentation,and composition and functions of the rumen bacterial community,leading to enhanced lactic acid production in Japanese Black beef cattle during fattening[J].PLoS One,2019,14(11):e0225448.
[3] ARIK H D,GULSEN N,HAYIRLI A,et al.Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in cattle[J].Journal of Animal Physiology and Animal Nutrition,2019,103(2):416-426.
[4] ELGHANDOUR M M Y,TAN Z L,HAFSA S H A,et al.Saccharomyces cerevisiae as a probiotic feed additive to non and pseudo-ruminant feeding:A review[J].Journal of Applied Microbiology,2020,128(3):658-674.
[5] DU H G,KHAS E,SHENGYANG C,et al.Correlation of the rumen fluid microbiome and the average daily gain with a dietary supplementation of Allium mongolicum Regel extracts in sheep1[J].Journal of Animal Science,2019,97(7):2865-2877.
[6] NIU Y,MENG Q,LI S,et al.Effects of diets supplemented with ensiled mulberry leaves and sun-dried mulberry fruit pomace on the ruminal bacterial and archaeal community composition of finishing steers[J].PLoS One,2016,11(6):e0156836.
[7] AROWOLO M A,HE J H.Use of probiotics and botanical extracts to improve ruminant production in the tropics:A review[J].Animal Nutrition,2018,4(3):241-249.
[8] 戴维,班博,方热军.微生态制剂的作用机理及其在畜牧生产方面的应用[J].湖南饲料,2020,3:30-32. DAI W,BAN B,FANG R J.Mechanism of microecological agents and its application in animal husbandry[J].Hunan Feed,2020,3:30-32.(in Chinese)
[9] ZHANG Y,YOSHIDA M,VADLANI P V.Biosynthesis of D-lactic acid from lignocellulosic biomass[J].Biotechnology Letters,2018,40(8):1167-1179.
[10] 张薇.乳酸的来源和生成途径研究及¹³C对¹³C标记化合物质谱行为的影响[D].杭州:浙江大学,2017. ZHANG W.Study on the origins and generation pathway of lactic and effects of ¹³C on ionization and fragmentation of ¹³C-labeled compounds[D].Hangzhou:Zhejiang University,2017.(in Chinese)
[11] 邵子奇.浅谈乳酸的产生及其对运动的影响[J].科技资讯,2018,16(25):231-232. SHAO Z Q.Production of lactic acid and its influence on sports[J].Science & Technology Information,2018,16(25):231-232.(in Chinese)
[12] 蔡娟,余英英,臧长江,等.反刍动物瘤胃乳酸代谢及其调控的研究进展[J].中国饲料,2019,9:5-9. CAI J,YU Y Y,ZANG C J,et al.Research progress of rumen lactic acid metabolism and its regulation in ruminants[J].Chinese Feed,2019,9:5-9.(in Chinese)
[13] DURGADEVI A,AAMOD N,SHARAD L,et al.Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system[J].Veterinary World,2019,12(9):1362-1371.
[14] 陈连民,王洪荣.瘤胃中乳酸的代谢及其调控机制[J].草业科学,2016,5:972-980. CHEN L M,WANG H R.Advances in the metabolism and regulation of lactic acids in the rumen[J].Pratacultural Science,2016,5:972-980.(in Chinese)
[15] LUO J,RANADHEERA C S,KING S,et al.Potential influence of dairy Propionibacteria on the growth and acid metabolism of Streptococcus bovis and Megasphaera elsdenii[J].Beneficial Microbes,2017,8(1):111-119.
[16] 祖菲,段纲,项勋,等.牛链球菌的分离鉴定及生物信息学分析[J].中国畜牧兽医,2015,42(11):2902-2908. ZU F,DUAN G,XIANG X,et al.Isolation,identification and bioinformatics analysis of Streptococcus bovis[J].Chinese Journal of Animal Husbandry & Veterinary Medicine,2015,42(11):2902-2908.(in Chinese)
[17] JOSHUA C M,LUAN S Y,FELIPE C C,et al.Induction of subacute ruminal acidosis affects the ruminal microbiome and epithelium[J].Frontiers in Microbiology,2016,7:701.
[18] GARRETT R O.Diagnosis and management of subacute ruminal acidosis in dairy herds[J].The Veterinary Clinics of North America.Food Animal Practice,2017,33(3):463-480.
[19] 王洪荣.反刍动物碳水化合物代谢利用机制及消化道健康的系统营养调控研究进展[J].动物营养学报,2020,32(10):4686-4696. WANG H R.Research advances in system-nutritional regulation on mechanism of carbohydrates metabolism and gastrointestinal health of ruminants[J].Chinese Journal of Animal Nutrition,2020,32(10):4686-4696.(in Chinese)
[20] 汤利民,王文丹,苏衍菁,等.预防亚急性瘤胃酸中毒的营养调控措施[J].中国奶牛,2019,4:9-13. TANG L M,WANG W D,SU Y Q,et al.Nutritional control measures to prevent subacute gastric acidosis[J].China Dairy Cattle,2019,4:9-13.(in Chinese)
[21] 刘强.盐穗木提取物对瘤胃微生物影响及抑菌成分鉴定[D].阿拉尔:塔里木大学,2019. LIU Q.Effect of Salicornia europaea extract on rumen microorganisms and identification of antibacterial components[D].Alear:Tarim University,2019.(in Chinese)
[22] 陈连民.山羊瘤胃中主要乳酸代谢菌代谢机制及其调控瘤胃酸中毒的应用研究[D].扬州:扬州大学,2017. CHEN L M.Mechanism of lactate metabolism in rumen microbiota and effect of Megasphaera elsdenii on relieving SARA in goats fed high grain diets[D].Yangzhou:Yangzhou University,2017.(in Chinese)
[23] 贾琦珍,陈根元,邓利,等.小花棘豆中毒对和田羊瘤胃溶纤维丁酸弧菌的影响[J].西北农业学报,2015,24(12):40-45. JIA Q Z,CHEN G Y,DENG L,et al.Effect of oxytropis glabra DC poisoning on Butyrivibrio fibrivolen in rumen of Hetian sheep[J].Acta Agriculturae Boreali-occidentalis Sinica,2015,24(12):40-45.(in Chinese)
[24] 王尧悦,赵钊艳,王兴涛,等.日粮营养水平对150~180日龄滩羊瘤胃相关微生物菌群数量、pH和VFA含量的影响[J].畜牧兽医学报,2016,47(10):2060-2070. WANG Y Y,ZHAO Z Y,WANG X T,et al.Effect of dietary nutrient levels on the number of related microbes,pH and VFA levels in rumen of Tan sheep aged from 150 to 180 days[J].Chinese Journal of Animal and Veterinary Sciences,2016,47(10):2060-2070.(in Chinese)
[25] 严淑红.茶皂素对奶牛瘤胃微生物区系、瘤胃发酵及产奶性能的调控研究[D].北京:北京农学院,2016. YAN S H.Study on the regulation of tea saponin on rumen microorganisms of dairy cows,rumen fermentation and milk performance[D].Beijing:Beijing Agricultural College,2016.(in Chinese)
[26] XIA G L,ZHAO F F,WANG H G.Advances in lactic acids metabolism in rumen and regulation of ruminal acidosis for ruminants[J].Chinese Journal of Animal Nutrition,2019,31(4):1511-1517.
[27] LOUIS P,FLINT H J.Formation of propionate and butyrate by the human colonic microbiota[J].Environmental Microbiology,2017,19(1):29-41.
[28] 胡宝吉,薄禄龙,邓小明,等.乳酸酸中毒的相关研究进展[J].中国医药导报,2018,15(3):22-25. HU B J,BO L L,DENG X M,et al.Research progress on lactic acidosis[J].Chinese Medicine Guides,2018,15(3):22-25.(in Chinese)
[29] 王洪荣.反刍动物瘤胃酸中毒机制解析及其营养调控措施[J].动物营养学报,2014,26(10):3140-3148. WANG H R.Mechanism analysis and nutrition strategies for prevention of sub-acute ruminal acidosis in ruminants[J].Journal of Animal Nutrition,2014,26(10):3140-3148.(in Chinese)
[30] 李鹏,刘敏跃,于立辉,等.瘤胃酸中毒的微生物学研究进展[J].饲料工业,2013,34(23):58-61. LI P,LIU M Y,YU L H,et al.Research progress in microbiology of gastric cancer acidosis[J].Feed Industry,2013,34(23):58-61.(in Chinese)
[31] JONATHAN C D,NATHAN R R,MARK F M,et al.The influence of Megasphaera elsdenii on rumen morphometrics of cull cows immediately stepped up to a high-energy finishing diet[J].Translational Animal Science,2020,4(1):194-205.
[32] YOHE T T,ENGER B D,WANG L,et al.Short communication:Does early-life administration of a Megasphaera elsdenii probiotic affect long-term establishment of the organism in the rumen and alter rumen metabolism in the dairy calf?[J].Journal of Dairy Science,2018,101(2):1747-1751.
[33] LIANMIN C,YIZHAO S,CHAO W,et al.Megasphaera elsdenii lactate degradation pattern shifts in rumen acidosis models[J].Frontiers in Microbiology,2019,10:162.
[34] ZAIAT H M E,KHOLIF A E,MOHAMED D A,et al.Enhancing lactational performance of Holstein dairy cows under commercial production:Malic acid as an option[J].Journal of the Science of Food and Agriculture,2019,99(2):885-892.
[35] RIE N,YO-HAN K,AKIRA O,et al.Effects of repeated subacute ruminal acidosis challenges on the adaptation of the rumen bacterial community in Holstein bulls[J].Journal of Dairy Science,2018,101(5):4424-4436..
[36] 武心镇,高玉霞,欧海龙,等.奶牛瘤胃中反刍兽月形单胞菌的分离鉴定[J].动物医学进展,2008,10:25-28. WU X Z,GAO Y X,OU H L,et al.Isolation and identification of ruminant luna in rumen of cow[J].Progress in Veterinary Medicine,2008,10:25-28.(in Chinese)
[37] MILLS J A N,CROMPTON L A,ELLIS J L,et al.A dynamic mechanistic model of lactic acid metabolism in the rumen[J].Journal of Dairy Science,2014,97(4):2398-2414.
[38] 陈婧.乳酸利用菌对奶牛瘤胃消化代谢及生产性能的影响[D].太谷:山西农业大学,2019. CHEN J.Effects of lactic acid utilizing bacteria on rumen digestion and metabolism and productive performance of dairy cows[D].Taigu:Shanxi Agricultural University,2019.(in Chinese)
[39] HUGE F M,ANTONGIO P F.Ruminal acidosis,bacterial changes,and lipopolysaccharides[J].Journal of Animal Science,2020,98(8):skaa248.
[40] VILLOT C,MEUNIER B,BODIN J,et al.Relative reticulo-rumen pH indicators for subacute ruminal acidosis detection in dairy cows[J].Animal,2018,12(3):481-490.
[41] SATO S.Pathophysiological evaluation of subacute ruminal acidosis (SARA) by continuous ruminal pH monitoring[J].Animal Science Journal,2016,87(2):168-177.
[42] 张瑞阳.组学技术研究亚急性瘤胃酸中毒对奶牛瘤胃微生物、代谢和上皮功能的影响[D].南京:南京农业大学,2015. ZHANG R Y.Omics-based approaches to assess the effects of subacute ruminal acidosis on rumen microbiota,metabolism and epithelial function in dairy cows[D].Nanjing:Nanjing Agricultural University,2015.(in Chinese)
[43] 包维臣,崔景丽,姚国强,等.奶牛瘤胃和肠道微生态系统中乳酸菌的种类及益生机理[J].中国奶牛,2014,17:21-25. BAO W C,CUI J L,YAO G Q,et al.The diversity and probiotic mechanism of lactic acid bacteria in rumen and gasintestinal tract of of dairy cows[J].Chinese Dairy Cattle,2014,17:21-25.(in Chinese)
[44] 孙劼.植物提取物对反刍动物瘤胃酸中毒的调控作用[J].中国饲料,2017,16:16-19. SUN J.Regulation of plant extracts on rumen acidosis[J].Chinese Feed,2017,16:16-19.(in Chinese)
[45] 范曜天,孙劼,夏光亮,等.添加槐花、香蕉花和杭白菊对体外诱导人工瘤胃酸中毒缓解效果的研究[J].动物营养学报,2019,31(10):4757-4765. FAN Y T,SUN J,XIA G L,et al.Alleviating effects of adding Sophora japonica,Musa nana flower and Chrysanthemum morifolium on induced artificial rumen acidosis in vitro[J].Chinese Journal of Animal Nutrition,2019,31(10):4757-4765.(in Chinese)
[46] 郭善军.红藻提取物对体外瘤胃发酵特性、产甲烷和微生物生长的影响[J].中国饲料,2020,6:54-57. GUO S J.Effects of red algae extract on rumen in vitro fermentation characteristics,methanogenesis and microbial growth[J].China Feed,2020,6:54-57.(in Chinese)
[47] 张芮铭,田可,何天乐,等.植物提取物对高精料饲粮下反刍动物瘤胃功能、微生物区系和炎症反应的调控效果[J].动物营养学报,2020,32(1):36-41.(in Chinese) ZHANG R M,TIAN K,HE T L,et al.Regulation effects of plant extracts on rumen function,microflora and inflammatory response of ruminants under high-concentrate diet[J].Chinese Journal of Animal Nutrition,2020,32(1):36-41.
[48] 杨艳,瞿明仁,王荣民,等.反刍动物瘤胃酸中毒研究进展[J].江西畜牧兽医杂志,2020,3:4-7. YANG Y,QU M R,WANG R M,et al.Research progress of rumen acidosis in ruminants[J].Jiangxi Journal of Animal Husbandry & Veterinary Medicine,2020,3:4-7.(in Chinese)
[49] DAI X X,HACKMANN T J,LOBO R R,et al.Lipopolysaccharide stimulates the growth of bacteria that contribute to ruminal acidosis[J].Applied and Environmental Microbiology,2020,86(4):e02193-19.
[50] WATANABE Y,KIM Y,KUSHIBIKI S,et al.Effects of active dried Saccharomyces cerevisiae on ruminal fermentation and bacterial community during the short-term ruminal acidosis challenge model in Holstein calves[J].Journal of Dairy Science,2019,102(7):6518-6531.
[51] 高彩霞,吴正钧.L-乳酸、D-乳酸对3种食源性致病菌的抑制作用[J].乳业科学与技术,2014,37(5):1-3. GAO C X,WU Z J.Antibacterial activity of L-lactic acid and D-lactic acid aganist three foodborne pathogenic bacteria[J].Journal of Dairy Science and Technology,2014,37(5):1-3.(in Chinese)
[52] DING Z K,XU Y Q.Lactic acid is absorbed from the small intestine of sheep[J].Journal of Experimental Zoology,2003,295(1):29-36.
[53] 王凤婷,靳盼盼,刘芳,等.乳酸对粪肠球菌的抑菌作用及作用机制[J].江苏农业学报,2018,34(1):200-206. WANG F T,JIN P P,LIU F,et al.Antibacterial activity and mechanism of lactic acid on Enterococcus faecalis[J].Jiangsu Journal of Agricultural Sciences,2018,34(1):200-206.(in Chinese)
[54] 贺美玲.酸马奶源乳酸杆菌代谢物对犊牛肠黏膜屏障作用的研究[D].呼和浩特:内蒙古农业大学,2019. HE M L.Mechanism of metabolites of Lactobacillus from mare's milk on intestinal mucosal barrier in calf[D].Hohhot:Inner Mongolia Agricultural University,2019.(in Chinese)
[55] 苏文伟.乳酸等指标在奶牛分娩前后的动态变化及相关性研究[D].南宁:广西大学,2008. SU W W.Study on dynamic changes and correlation of lactate and other indicators before and after delivery[D].Nanning:Guangxi University,2008.(in Chinese)
[56] LEONHARD G,ANNABELLA K,ELKE H,et al.Long-term influence of feeding barley treated with lactic acid and heat on performance and energy balance in dairy cows[J].Archives of Animal Nutrition,2017,71(1):54-66.
[57] 孙德成,赵智力,魏曼琳,等.复合脂肪酸和乳酸对奶牛瘤胃VFA和CH₄的影响[J].中国奶牛,2011,16:8-11. SUN D C,ZHAO Z L,WEI M L,et al.Effects of lactic acid and fatty acids on Holsteins rumen volatile fatty acids and methane[J].China Dairy Cattle,2011,16:8-11.(in Chinese)
[58] 白永亮,凌志洲,林燕丹,等.乳酸杆菌作用下香蕉抗性淀粉的体外发酵研究[J].食品研究与开发,2018,39(7):199-204. BAI Y L,LIN Z Z,LIN Y D,et al.In vitro fermentation of banana resistant starch by Lactobacillus[J].Food Research and Development,2018,39(7):199-204.(in Chinese)
[59] 冯尚连,尚建钢,孙玥滢,等.乳酸对仔猪肠道大肠杆菌和乳酸杆菌的影响[J].浙江农业学报,2011,23(3):506-510. FENG S L,SHANG J G,SUN Y Y,et al.Effects of lactic acid on intenstinal E.coli and Lactobacillus of piglets[J].Acta Agriculturae Zhejiangensis,2011,23(3):506-510.(in Chinese)
[60] AMETAJ B N,IQBAL S,SELAMI F,et al.Intravaginal administration of lactic acid bacteria modulated the incidence of purulent vaginal discharges,plasma haptoglobin concentrations,and milk production in dairy cows[J].Research in Veterinary Science,2014,96(2):365-70.
[61] 曾佳佳,陈静,陈振,等.腐植酸与乳酸杆菌联合使用对奶牛产奶量和乳品质的影响[J].中国饲料,2018,9:38-41. ZENG J J,CHEN J,CHEN Z,et al.Effect of humic acid combined with Lactobacillus on milk yield and milk quality of dairy cows[J].China Feed,2018,9:38-41.(in Chinese)
[62] ZHANG R,DONG X,ZHOU M,et al.Oral administration of Lactobacillus plantarum and Bacillus subtilis on rumen fermentation and the bacterial community in calves[J].Animal Science Journal,2017,88(5):755-762.
[63] DALANEZI F M,JOAQUIM S F,GUIMARAES F F,et al.Influence of pathogens causing clinical mastitis on reproductive variables of dairy cows[J].Journal of Dairy Science,2020,103(4):3648-3655.
[64] CHOTIGARPA R,LAMPANG K N,PIKULKAEW S,et al.Inhibitory effects and killing kinetics of lactic acid rice gel against pathogenic bacteria causing bovine mastitis[J].Scientia Pharmaceutica,2018,86(3):E29.
[65] 宋月,李柏良,李娜,等.1株抑制乳房炎病原菌乳杆菌的筛选、鉴定及其抑菌机理研究[J].食品工业科技,2019,40(20):120-126. SONG Y,LI B L,LI N,et al.Screening and identification of lactic acid bacteria for inhibiting bovine mastitis and preliminary study of antibacterial mechanism[J].Science and Technology of Food Industry,2019,40(20):120-126.(in Chinese)
[66] 吴琼.乳酸杆菌减轻大肠杆菌诱发奶牛乳腺上皮细胞炎性损伤的机理[D].北京:中国农业大学,2018. WU Q.Lactobacillus attenuates inflammatory damage of bovine mammary epithelial cells infected with Escherichia coli[D].Beijing:China Agricultural University,2018.(in Chinese)

Research Progress on Metabolic Pathway of Lactic Acid and Its Effect on the Health of Dairy Cows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Jinghong, MA Hui, LIU Jintao, MIAO Renfang, LIU Hongzhi, LIU Duncheng, QU Yongli. Effects of Supplemental Light on Blood Endocrine Hormone Concentrations and Circadian Rhythm Changes in Late-gestation Holstein Breeding Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3178-3189.
[2]	WANG Xueyang, LIN Zhan, LI Jiachen, XI Peng, ZHANG Hewei. Progress on Relationship Between Plant Extracts and Horizontal Transfer of Antibiotic Resistance Genes Carried by Plasmids [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3479-3488.
[3]	WANG Haotian, LI Junzhe, MEI Xiaoying, LI Longxian, LI Yingying, ZHANG Mingrui, WU Yi. Effects of Cuscuta chinensis and Arctium lappa Compound Plant Extracts on Urine Physicochemical,Serum Antioxidant and Immune Indexes in Adult Cats [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2531-2539.
[4]	LIU Zhixu, HAN Wenchang, LI Yanqin, ZHANG Liwen, LIU Yan, YUAN Shaowei, LIJianbin, WANG Xiao, CAO Rongfeng. Study on the Correlation Between β-lactase blaZ Gene and Microorganisms in Milk of Cows with Latent Mastitis in Shandong Region [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2318-2327.
[5]	SUN Jintao, GU Xinshu, WANG Jinquan, WANG Xiumin, TAO Hui, WANG Zhenlong, HAN Bing. Mechanisms and Recent Advances of Lactic Acid Bacteria in Treating Intestinal Inflammation in Companion Animals [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2442-2448.
[6]	HAN Yurun, SHI Yuangang, KANG Xiaolong. Influencing Factors of Ruminating Behavior and the Application of Intelligent Monitoring Methods in Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1554-1566.
[7]	LIU Tuo, CHENG Zhiqiang, CHENG Zhe, ZANG Changjiang, NAN Bingyu, LI Tao, LI Xiaobin, LI Yunmeng, QIU Yaqi, YAN Fayu, WU Hairong, YUAN Dong, WANG Yongli, LIU Wentao. Effects of Nicotinamide on Rumen Fermentation Parameters,Microbial Protein Yield and Microflora in Lactating Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 593-604.
[8]	YU Tianli, HE Zhenlian, HAN Yijing, REN Jingyu, XIA Chengqiang, PEI Caixia. Effects of Adding Polygonum hydropiper L.to High-concentrate Diet on Fermentation Parameters and Flora of Rumen in Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 205-214.
[9]	WAN Baoxia, MENG Lingying, SUN Siyu, ZHAO Yujie, WANG Jiaqi, WANG Qiuju. Analysis of Drug Resistance Genes and Biological Characterization of Goose Derived Bacillus and Lactic Acid Bacteria [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 351-363.
[10]	LI Jinnan, WANG Yahui, DENG Tianyu, LIANG Mang, DU Lili, LI Keanning, XUE Qingqing, QIAN Li, GAO Xue, ZHANG Lupei, ZHU Bo, CHEN Yan, WANG Zezhao, LI Junya, GAO Huijiang. Cloning,Bioinformatics Analysis and Tissue Expression Profile of Coding Region of Bovine MED28 Gene [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3225-3236.
[11]	YAO Weijia, LUO Chunhai, LIU Jiajin, WANG Wei, LI Danyang, LIU Bingqi, FU Shixin. Effect of Overexpression of miRNA-424-5p Targeting AKT3 on Apoptosis of Endometrial Epithelial Cells in Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3635-3642.
[12]	HE Xingli, ZHOU Peiyao, ZHANG Xiaoxue, MOU Quanzhou, LI Yang, WANG Zhaoyuan, LIU Peng, WANG Zi, SONG Yangyang, LI Xiaolin, SHEN Binglei. Effect of Staphylococcus aureus and Staphylococcus epidermidis on the Secretion of Inflammatory Factors by Neutrophils in Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3676-3686.
[13]	JI Guoshang, SHENG Hui, ZHANG Junxing, FENG Xue, HU Chunli, WANG Yachun, MA Yanfen, LI Li, YANG Wenfei, MA Yun. Study on the Function of PCYOX1L Gene Regulating LPS-induced Bovine Endometrial Epithelial Cells [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2984-2997.
[14]	MA Xiaoxue, LONG Rui, NIU Yujie, GUO Hongyong, LUO Ruiqing, WU Yanyan, ZHANG Wenju. Effect of Adding Isoacids in Perinatal Diet on Rumen Fermentation Parameters, Rumen Microorganisms and Production Performance of Dairy Cows [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1428-1437.
[15]	ZHANG Tenglong, GUO Chenyang, ZHONG Huachen, LIU Jialin, SONG Jie, WANG Lifang. Effects of Dandelion and Forsythia Extracts on Production Performance,Serum Antioxidant and Immune Indexes of Dairy Cows With Subclinical Mastitis [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1490-1499.