China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (1): 45-50.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the Complete Genome of Porcine Circovirus Type 2 Gansu Lanzhou Strain

JIA Huai-jie1,2, JING Zhi-zhong1, FANG Yong-xiang1, WANG Xiao-xia1, CHEN Guo-hua1, LI Ke-bin1,2, DOU Yong-xi1, QU Hui-ling1,2,3   

  1. (1.State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS; Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou 730046, China; 2.Veterinary College of Gansu Agricultural University, Lanzhou 730070, China; 3.Veterinary Bureau of Gansu Province, Lanzhou 730030, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
  • Contact: JING Zhi-zhong

Abstract: Two pairs of primers were designed and synthesized according to the full length genome of PCV2 in GenBank. Genomic DNA was extracted from samples of suspected post weaning multisystemic wasting syndrome (PMWS) pig in Lanzhou, Gansu province, and the full length genome of PCV2 was fragmentation cloned by PCR into pGEM-T easy vector, recombinant vector pGEM-PCV2 were constructed and then sequenced. Sequence analysis with DNAStar software showed that the genome of PCV2 Gansu Lanzhou strain is 1767 bp and the result of homology comparison of the full length genome among strains published in GenBank, indicated that it displayed high nucleotide and amino acid identities with ZhouKou (EU656143) strain (99.4% nucleotide identity and 98.6% amino acid identity), but distinct from GS03 (EU547458) which was another Lanzhou epidemic strain. So strain in this test may be a variant strain (GenBank accession No. FJ447482) prevalent in Lanzhou, Gansu.

Key words: porcine circovirus type 2(PCV2); genome; cloning; sequence analysis

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