抗猪圆环病毒2型Cap蛋白单克隆抗体杂交瘤细胞株的建立

Abstract

Abstract: These McAbs would be useful tools for diagnostic of the PCV2 virus. BALB/c mice were immunized with the purified recombinant capsid(pET-Cap) protein of porcine circovirus type 2 (PCV2) prepared from Escherichia expression system. Two monoclonal antibodies (McAbs) designated 3D5 and 5C5 were obtained. The titers of two strain McAb in cell supernatant was 1∶2048,1∶1024 and that of ascites was 1∶204800,1∶102400 respectively by indirect ELISA,the average number of chromosome were 95,96 respectively,two McAbs were confirmed to have the ability to PCV2 by Western blotting.Strain 3D5,5C5 hybridoma cell could also stably secret McAb after twenty serial passages and freezing and reviving three times.

Key words: porcine circovirus type 2; recombinant Cap protein; monoclonal antibodies

CLC Number:

S852.65⁺9.2

QIAO Hong-xing;BIAN Chuan-zhou;ZHANG Xiao-gen. Preparation of Monoclonal Antibody to the Recombinant Capsid of Porcine Circovirus Type 2[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(6): 163-165.

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Preparation of Monoclonal Antibody to the Recombinant Capsid of Porcine Circovirus Type 2

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