牛病毒性腹泻病毒RT-PCR检测方法的建立及应用

Abstract

Abstract: A reverse transcription PCR (RT-PCR) assay for detection of bovine viral diarrhea virus (BVDV)was established using a pair of specific primers of BVDV. This method could specifically amplify a 471 bp fragment from BVDV Oregon C24V strain,but not from classical swine fever virus (CSFV),infectious bovine rhinotracheitis virus (IBRV),bovine respiratory syncytial virus (BRSV),parainfluenza virus-3 (PI3) and normal MDBK cells. The organs were detected by RT-PCR and virus isolated that collected from a sick calf with bovine viral diarrhea (BVD) like syndrome.These results indicated that the established RT-PCR assay was specific,sensitive,efficient and rapid for detection,monitoring and epidemiological investigation of BVDV infection.

Key words: bovine viral diarrhea virus; RT-PCR; detection

CLC Number:

ZHANG Shu-qin;GUO Li;ZHANG Ting-ting;WU Yong-wang;WU Hua. Establishment and Application of a RT-PCR Assay for Detection of Bovine Viral Diarrhea Virus[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(6): 159-162.

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Establishment and Application of a RT-PCR Assay for Detection of Bovine Viral Diarrhea Virus

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