4-萜烯醇对大肠杆菌的抗菌作用研究

doi:10.16431/j.cnki.1671-7236.2025.09.044

Abstract

Abstract: 【Objective】 The experiment aimed to evaluate the antibacterial activity of terpinen-4-ol and provide support for its structural optimization and clinical application. 【Method】 Escherichia coli (E.coli) was selected as the model bacterium in this study.The minimum inhibitory concentration (MIC) of terpinen-4-ol against E.coli was determined by double dilution method.The antibacterial curve was measured by D_{600 nm} value.The effects of terpinen-4-ol on cell morphology of E.coli were analyzed by scanning electron microscopy.Then,the effect of terpinen-4-ol on E.coli cell membrane was evaluated by measuring the concentration of extracellular alkaline phosphatase (ALP) and β-galactosidase.Simultaneously,the leakage of nucleic acid and protein was determined using ultra micro spectrophotometer.The interaction of terpinen-4-ol with E.coli DNA was detected by nucleic acid gel electrophoresis. 【Result】 The MIC of terpinen-4-ol against E.coli was 0.013 mol/L.The antibacterial effect of terpinen-4-ol on E.coli was dose-dependent,and 1 MIC of terpinen-4-ol had good antibacterial effect within 24 h.E.coli treated with terpinen-4-ol became swelling and deformation,rough surface,and adhesion between bacterial bodies.Compared with control group,0.5 MIC of terpinen-4-ol could lead to a significant increase in the content of ALP in the supernatant of E.coli (P＜0.05) and an extremely significant increase in the content of β-galactosidase (P＜0.01),and the increase was positively correlated with the concentration of terpinen-4-ol.Compared with control group,0.5 MIC of terpinen-4-ol could also increase the contents of proteins and nucleic acids in the supernatant of E.coli,and it was positively correlated with the concentration of terpinen-4-ol and the duration of action.There was no binding between terpinen-4-ol and the E.coli DNA. 【Conclusion】 Terpinen-4-ol had a good antibacterial effect on E.coli,and exerted antibacterial effects by disrupting the permeability of cell membranes,and then leading to leakage of cellular contents and bacterial death,which laid a foundation for further research on the antibacterial mechanism of terpinen-4-ol on E.coli.

Key words: terpinen-4-ol; Escherichia coli; cell membrane; antibacterial effect

CLC Number:

ZHOU Lingling, WANG Yuhang, ZHANG Yuanchen, ZHANG Mingliang, LIAN Kaiqi. Study on Antimicrobial Effects of Terpinen-4-ol Against Escherichia coli[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4497-4504.

References

[1] 岳康,郭延生,刘红燕,等.银川地区肉牛子宫内膜炎病原菌的分离鉴定与药敏试验[J].动物医学进展,2023,44(10):119-123. YUE K,GUO Y S,LIU H Y,et al.Isolation,identification and drug fusceptibility test of endometritis pathogens in beef cattle in Yinchuan area[J].Progress in Veterinary Medicine,2023,44(10):119-123.(in Chinese)
[2] 郭文洁,高丹,王若琪,等.噬菌体疗法在动物细菌感染中的应用研究进展[J].黑龙江畜牧兽医,2023，20:29-34. GUO W J,GAO D,WANG R Q,et al.Research progress of application of phage therapy for bacterial infections in animals[J].Heilongjiang Animal Science and Veterinary Medicine,2023,20:29-34.(in Chinese)
[3] 王恬,张昊.饲用抗生素替代物在畜禽生产上的研究进展[J].饲料工业,2023,44(12):1-15. WANG T,ZHANG H.Research progress of feed antibiotic substitutes on livestock and poultry production[J].Feed Industry,2023,44(12):1-15.(in Chinese)
[4] 黄立标,冯锦东,王德立,等.海南产肉豆蔻叶挥发油及其主要成分动态变化研究[J].中成药,2010,32(12):2126-2130. HUANG L B,FENG J D,WANG D L,et al.Dynamic changes of volatile oil and major constituents from leaves of Myristica fragrans Houtt in Hainan province[J].Chinese Traditional Patent Medicine,2010,32(12):2126-2130.(in Chinese)
[5] 高海荣,王亚鑫,谢晨,等.三种蒿类植物挥发油成分及抗菌活性的比较分析[J].现代食品科技,2020,36(1):262-268. GAO H R,WANG Y X,XIE C,et al.Comparison and analysis of chemical components and their antimicrobial activity of essential oils from three Artemisia plants[J].Modern Food Science and Technology,2020,36(1):262-268.(in Chinese)
[6] 黄德浩,吴伟超,吴国兴,等.茶树精油抗炎抗菌功效的研究现状[J].今日药学,2018,28(3):211-215. HUANG D H,WU W C,WU G X,et al.Research status of anti-inflammatory and anti-bacterial effect about tea tree oil[J].Pharmacy Today,2018,28(3):211-215.(in Chinese)
[7] 李晓微,陈君,王义明,等.气相色谱同时测定鱼腥草注射液中三种成分的含量[J].中药材,2011,34(5):808-810. LI X W,CHEN J,WANG Y M,et al.Simultaneous determination of three components in Houttuynia cordata injection by gas chromatography[J].Journal of Chinese Medicinal Materials,2011,34(5):808-810.(in Chinese)
[8] YU D,WANG J,SHAO X,et al.Antifungal modes of action of tea tree oil and its two characteristic components against Botrytis cinerea[J].Journal of Applied Microbiology,2015,119(5):1253-1262.
[9] TERZI V,MORCIA C,FACCIOLI P,et al.In vitro antifungal activity of the tea tree (Melaleuca alternifolia) essential oil and its major components against plant pathogens[J].Letters in Applied Microbiology,2007,44(6):613-618.
[10] HART P,BRAND C,CARSON C,et al.Terpinen-4-ol,the main component of the essential oil of Melaleuca alternifolia (tea tree oil),suppresses inflammatory mediator production by activated human monocytes[J].Inflammation Research,2000,49(11):619-626.
[11] LIU Y,XU L,DU H,et al.Effects of adding tea tree oil on growth performance,immune function,and intestinal function of broilers[J].Poultry Science,2023,102(11):102936.
[12] BO R,ZHAN Y,WEI S,et al.Tea tree oil nanoliposomes:Optimization,characterization,and antibacterial activity against Escherichia coli in vitro and in vivo[J].Poultry Science,2023,102(1):102238.
[13] 连凯琪,孟蕊,王梦婷,等.4-萜烯醇对沙门菌的抗菌机制[J].中国兽医学报,2023,43(8):1684-1689. LIAN K Q,MENG R,WANG M T,et al.Antibacterial mechanism of terpinen-4-ol against Salmonella[J].Chinese Journal of Veterinary Science,2023,43(8):1684-1689.(in Chinese)
[14] 连凯琪,孟祥凤,牛维东,等.4-萜稀醇对金黄色葡萄球菌的抑菌机制[J].中国兽医学报,2022,42(7):1438-1442. LIAN K Q,MENG X F,NIU W D,et al.Antimicrobial mechanismof terpinen-4-ol against Staphylococcus aureus[J].Chinese Journal of Veterinary Science,2022,42(7):1438-1442.(in Chinese)
[15] GUIMARÃES A C,MEIRELES L M,LEMOS M F,et al.Antibacterial activity of terpenes and terpenoids present in essential oils[J].Molecules,2019,24(13):2471.
[16] ZHANG H,LI X,WANG Y,et al.Mechanistic insights into the antibacterial effects of 4-terpineol against Escherichia coli O157∶H7[J].International Journal of Food Microbiology,2020,332:108589.
[17] GAO F,ZHOU H,SHEN Z,et al.Long-lasting anti-bacterial activity and bacteriostatic mechanism of tea tree oil adsorbed on the amino-functionalized mesoporous silica-coated by PAA[J].Colloids and Surface B-Biointerfaces,2020,188:110784.
[18] LI W,LI H,SHI Q,et al.The dynamics and mechanism of the antimicrobial activity of tea tree oil against bacteria and fungi[J].Applied Microbiology and Biotechnology,2016,100(20):8865-8875.
[19] ZHOU L,LIAN K,WANG M,et al.The antimicrobial effect of a novel peptide LL-1 on Escherichia coli by increasing membrane permeability[J]. BMC Microbiology,2022,22(1):220.
[20] 连凯琪,王宇航,曾雅静,等.抗菌肽LL-1对肺炎克雷伯菌ATCC 700603的抗菌机制[J].中国畜牧兽医,2024,51(5):2237-2244. LIAN K Q,WANG Y H,ZENG Y J,et al.Antimicrobial mechanism of antimicrobial peptide LL-1 against Klebsiella pneumoniae ATCC 700603[J].China Animal Husbandry & Veterinary Medicine,2024,51(5):2237-2244.(in Chinese)
[21] ZHANG F,YANG J,ZHAN Q,et al.Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions[J].Journal of Animal Science and Biotechnology,2023,14(1):77.
[22] HOSSEINZADEH S,SHARIATMADARI F,KARIMI TORSHIZI M,et al.Plectranthus amboinicus and rosemary (Rosmarinus officinalis L.) essential oils effects on performance,antioxidant activity,intestinal health,immune response,and plasma biochemistry in broiler chickens[J].Food Science & Nutrition,2023,11(7):3939-3948.
[23] ATTIA Y,AL-HARTHI M,EL-KELAWY M.Utilisation of essential oils as a natural growth promoter for broiler chickens[J].Italian Journal of Animal Science,2019,18(1):1005-1012.
[24] ATTIA Y,BAKHASHWAIN A,BERTU N.Thyme oil (Thyme vulgaris L.) as a natural growth promoter for broiler chickens reared under hot climate[J].Italian Journal of Animal Science,2017,16(2):275-282.
[25] ATTIA Y,AL-HARTHI M.Nigella seed oil as an alternative to antibiotic growth promoters for broiler chickens[J].European Poultry Science,2015,79(80):1.

Study on Antimicrobial Effects of Terpinen-4-ol Against Escherichia coli

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