China Animal Husbandry & Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (9): 4334-4345.doi: 10.16431/j.cnki.1671-7236.2025.09.030

• Preventive Veterinary Medicine • Previous Articles    

Isolation,Identification and Biological Characteristics Analysis of a Strain of Atlantibacter hermannii Derived from Black Bear

ZHANG Mengna1,2, XU Lili3, YANG Yajun2, LIU Ronghui2, WEI Yanwen2, WANG Qian2, TAN Zhenwei2, LYU Hongzhen2, ZHANG Lin2, GAO Min2, WANG Chengmin2,3,4, SUN Yongxue1   

  1. 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
    2. Institute of Zoology, Guangdong Academy of Sciences, Guangdong Key Laboratory of Animal Protection and Resource Utilization, Guangdong Public Laboratory of Wildlife Protection and Utilization, Guangzhou 510260, China;
    3. Nanling Wanze Institute of Microbial Engineering Co., Ltd., Wuhu 241300, China;
    4. U-Yibang Biotechnology (Shanghai) Co., Ltd., Shanghai 201103, China
  • Received:2025-01-22 Published:2025-08-29

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Abstract: 【Objective】 The purpose of this experiment was to screen strains with potential for livestock and poultry environmental restoration from Black bear feces,providing candidate strains for the development of livestock and poultry microecological preparations. 【Method】 Fresh Black bear feces were collected,and desulfurizing bacteria were initially screened using a selective medium.After isolation and purification,strains were identified via colony morphology and 16S rDNA sequencing.Growth characteristics and antimicrobial susceptibility of the isolated strain were analyzed.The biological properties were comprehensively characterized by evaluating its capacity for H2S removal,Cr(Ⅵ) passivation,plant growth promotion,and enzyme production. 【Result】 One strain of Atlantibacter hermanii was successfully isolated，and it was named M1633-2DR.On MHA solid medium,it formed yellow,circular colonies with smooth edges and a moist surface.Gram staining revealed Gram-negative,short bacilli.In MHB liquid medium,the strain entered the logarithmic growth phase at 1 h and the stationary phase at 16 h.The isolate was sensitive to 11 kinds of antibacterial drugs such as sulbactam and amikacin,and resistant to ampicillin.Desulfurization tests results showed that the removal rate of 160 mg/L S2- by the isolate reached 100% within 48 h,and the removal rates of 320,480 and 560 mg/L S2- were 62.23%,49.58% and 42.44%, respectively.The tolerance concentration of Cr(Ⅵ) of the isolate could reach 3 200 mg/L.The reduction rate of 50 mg/L Cr(Ⅵ) within 96 h was 100%,and the reduction rates of 150 and 250 mg/L Cr(Ⅵ) within 168 h were 48.23% and 37.78%,respectively.Further studies had found that this strain had the abilities of phosphorus solubilization (829.28 mg/L),nitrogen fixation (9.94 μg/mL),indoleacetic acid production (43.91 mg/L) and protease production (935.80 U/mL). 【Conclusion】 In this study,one strain of Atlantibacter hermanii was isolated and obtained.It had significant advantages in H2S removal,Cr(Ⅵ) reduction,plant growth promotion and protease production，and could be used as a candidate strain for environmental restoration in animal husbandry and the development of agricultural microbial preparations.

Key words: Atlantibacter hermannii; H2S; Cr(Ⅵ); biologicalfunction; protease

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