恒温扩增技术在鸟类性别鉴定中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2025.09.022

Abstract

Abstract: Birds play a crucial role in both natural ecosystems and human societies,and the technology for identifying bird sex is vital for ecological research and the sustainable development of animal husbandry.Traditional sex identification methods,such as anal swelling,are generally limited by the experience and proficiency of the operators,and by low efficiency.In recent years,molecular sex identification technology,including PCR,molecular markers technology,and isothermal amplification technology have advanced rapidly. These applications have improved the accuracy and simplicity of sex identification in birds.Especially the introduction of isothermal amplification technology has accelerated the development of the sex identification technology and become a new tool in the sex identification of birds.Compared with PCR and molecular markers techniques,isothermal amplification has shown significant advantages in bird sex identification with its high efficiency,specificity and simple operation.Isothermal amplification technology can complete sample amplification and reach the detection level in 30 to 60 minutes at a single temperature.The authors reviewed the conventional methods of sex identification,using loop-mediated isothermal amplification (LAMP),recombinase polymerase amplification (RPA)/recombinase-aided polymerase amplification (PAA),and cross-priming amplification (CPA) in sex of birds as examples,illustrating the progress of the different isothermal amplification techniques in the sex identification of birds,and providing reference for future related research.

Key words: isothermal amplification; sex identification; LAMP; RPA/PAA; CPA

CLC Number:

S813.3

TAN Shenluan, ZENG Fanwen, LI Li, WEI Hengxi, ZHANG Shouquan. Research Progress on Isothermal Amplification Technique in Sex Identification of Birds[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4237-4247.

References

[1] MACHKOUR-M’RABET S,TORRES-CRISTIANI L,CALM E S,et al.Sex identification and sex ratio of the American flamingo Phoenicopterus ruber using molecular techniques:Importance for management in zoological collections[J].Journal of Zoo and Aquarium Research,2024,12(1):16-22.
[2] LIU C W,HOU H Y,HSIEH H I,et al.Sex identification of birds in Taipei zoo[J].Zoo Biology,2024,43(3):268-275.
[3] SZÉKELY T,CARMONA-ISUNZA M C,ENGEL N,et al.The causes and implications of sex role diversity in shorebird breeding systems[J].IBIS-The International Journal of Avian Science,2024,166(2):357-385.
[4] HORKAEW P,KUPITTAYANANT S,KUPITTAYANANT P.Noninvasive in ovo sexing in Korat chicken by pattern recognition of its embryologic vasculature[J].Journal of Applied Poultry Research,2024,33(3):100424.
[5] SCHREUDER J,NIKNAFS S,WILLIAMS P,et al.Non-destructive prediction of fertility and sex in chicken eggs using the short wave near-infrared region[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2024,322:124716.
[6] 鲍丽菲.椰乡优品文昌鸡快慢羽自别雌雄配套系组配及其父本品系的初步选育[D].武汉:华中农业大学,2023. BAO L F.Combining two-way crossline for Yexiangyoupin Wenchang fowls autosexed by late vs.early feathering and initiative selective breeding of its sire-line[D].Wuhan:Huazhong Agricultural University,2023.(in Chinese)
[7] ANALLA M,FERNÁNDEZ RODRÍGUEZ P,MEDINA N,et al.Sexing eurasian eagle owls by external body and skeletal measurements[J].Journal of Field Ornithology,2022,93(4)：1.
[8] CUAN K,LI Z,ZHANG T,et al.Gender determination of domestic chicks based on vocalization signals[J].Computers and Electronics in Agriculture,2022,199:107172.
[9] NGOC L T N,LEE Y.Current trends in RNA virus detection via nucleic acid isothermal amplification-based platforms[J].Biosensors,2024,14(2):97.
[10] 王美青.鸭胚早期性别鉴定及性别决定候选基因表达的研究[D].扬州:扬州大学,2019. WANG M Q.Sex identification of early duck embryo and expression of candidate genes related to sex determination[D].Yangzhou:Yangzhou University,2019.(in Chinese)
[11] DOGLIERO A,ROSSI G,MAUTHE VON DEGERFELD M,et al.Comparison of celioscopy and histological examinations to assess male gonadal health and functionality in adults and immature wild raptors[J].Theriogenology,2017,102:139-146.
[12] HOMMA K,SIOPES T D,WILSON W O,et al.Identification of sex of day-old quail (Coturnix coturnix japonica) by cloacal examination1[J].Poultry Science,1966,45(3):469-472.
[13] 杨晶晶,韩闰凯,吴占福,等.基于CNN和图像深度特征的雏鸡性别自动鉴别方法[J].农业机械学报,2020,51(6):258-263. YANG J J,HAN R K,WU Z F,et al.Automatic recognition method of chick sex based on convolutional neural network and image depth features[J].Transactions of the Chinese Society for Agricultural Machinery,2020,51(6):258-263.(in Chinese)
[14] OTSUKA M,MIYASHITA O,SHIBATA M,et al.A novel method for sexing day-old chicks using endoscope system[J].Poultry Science,2016,95(11):2685-2689.
[15] 庄方明.吉林白鹅性别鉴定与卵黄囊形态差异及相关代谢基因在毛囊中表达规律的研究[D].长春:吉林农业大学,2023. ZHUANG F M.Sex identification and yolk sac morphology differences in Jilin White geese and the expression rule of related metabolic genes in feather follicles[D].Changchun:Jilin Agricultural University,2023.(in Chinese)
[16] 王梦磊,赵思琦,徐梦竹,等.孵化过程中鸡胚性别分化期的判定[J].甘肃农业大学学报,2024,59(2):17-23. WANG M L,ZHAO S Q,XU M Z,et al.Determination of the gender differentiation stage of chicken in the process of embryonic development[J].Journal of Gansu Agricultural University,2024,59(2):17-23.(in Chinese)
[17] NG C S,LI W.Genetic and molecular basis of feather diversity in birds[J].Genome Biology and Evolution,2018,10(10):2572-2586.
[18] XU X,WANG S,FENG Z,et al.Sex identification of feather color in geese and the expression of melanin in embryonic dorsal skin feather follicles[J].Animals,2022,12(11):1427.
[19] WIDELITZ R B,LIN G,LAI Y,et al.Morpho-regulation in diverse chicken feather formation:Integrating branching modules and sex hormone-dependent morpho-regulatory modules[J].Development,Growth & Differentiation,2019,61(1):124-138.
[20] LIN R L,CHEN H P,ROUVIER R,et al.Selection and crossbreeding in relation to plumage color inheritance in three Chinese egg type duck breeds (Anas platyrhynchos)[J].Animal Bioscience,2014,27(8):1069-1074.
[21] 庞有志,赵淑娟,张小辉,等.蛋用鹌鹑白羽基因座与黑羽基因座互作关系分析[J].东北农业大学学报,2022,53(7):28-34. PANG Y Z,ZHAO S J,ZHANG X H,et al.Interaction analysis of white feather locus and black feather locus in layer quail[J].Journal of Northeast Agricultural University,2022,53(7):28-34.(in Chinese)
[22] QI S,XU X,LIU L,et al.The development rule of feathers and application of hair root tissue in sex identification of Yangzhou geese[J].Poultry Science,2024,103(4):103529.
[23] GRIFFITHS R,DAAN S,DIJKSTRA C.Sex identification in birds using two CHD genes[J].Proceedings.Biological Sciences/The Royal Society,1996,263:1251-1256.
[24] ITOH Y,SUZUKI M,OGAWA A,et al.Identification of the sex of a wide range of Carinatae birds by PCR using primer sets selected from chicken EE0.6 and its related sequences[J].Journal of Heredity,2001,92(4):315-321.
[25] COUSTHAM V,GODET E,BEAUCLAIR L.A simple PCR method for sexing Japanese quail Coturnix japonica at hatching[J].British Poultry Science,2017,58(1):59-62.
[26] HOUCHEN C J,BERGMAN-GONZALEZ M,BUMANN E E.A novel qPCR-based technique for identifying avian sex:An illustration within embryonic craniofacial bone[J].The Journal of Genetics and Development 2024,62(1):e23530.
[27] PETROU E L,SCOTT L C,MCKEEMAN C M,et al.Molecular sexing of birds using quantitative PCR (qPCR) of sex-linked genes and Logistic regression models[J].Molecular Ecology Resources,2024,24(4):e13946.
[28] GRIFFITHS R,DOUBLE M C,ORR K,et al.A DNA test to sex most birds[J].Molecular Ecology,1998,7(8):1071-1075.
[29] KAHN N W,JOHN J S,QUINN T W.Chromosome-specific intron size differences in the avian CHD gene provide an efficient method for sex identification in birds [J].The Auk:Ornithological Advances,1998,115(4):1074-1078.
[30] FRIDOLFSSON A,ELLEGREN H.A simple and universal method for molecular sexing of non-ratite birds[J].Journal of Avian Biology,1999,30(1):116-121.
[31] LEE J C,TSAI L,HWA P,et al.A novel strategy for avian species and gender identification using the CHD gene[J].Molecular and Cellular Probes,2010,24(1):27-31.
[32] HILLEL J,GRANEVITZE Z,TWITO T,et al.Molecular markers for the assessment of chicken biodiversity[J].World’s Poultry Science Journal,2007,63(1):33-45.
[33] MORINHA F,CARVALHO M,FERRO A,et al.Molecular sexing and analysis of CHD1-Z and CHD1-W sequence variations in wild common quail (Coturnix c.coturnix) and domesticated Japanese quail (Coturnix c.japonica)[J].Journal of Genetics,2011，90:e39-e43.
[34] TUGIYANTI E,MUFTI M,PURWANTINI D.Sexual dimorphism and identification of single nucleotide polymorphism of growth hormone gene in Muscovy duck[J].Journal of the Indonesian Tropical Animal Agriculture,2017,42(3):167-174.
[35] GARCIA RAVENTÓS A,MUÑOZ MÉRIDA A,LAPIEDRA O,et al.Identification of sex-linked SNP markers in wild populations of monomorphic birds[J].Molecular Ecology Resources,2023,23(8):1905-1913.
[36] GLÖKLER J,LIM T S,IDA J,et al.Isothermal amplifications—A comprehensive review on current methods[J].Critical Reviews in Biochemistry and Molecular Biology,2021,56(6):543-586.
[37] 蔡文凯,蔡水淋,郝宗杰.几种主流恒温扩增技术及其优化策略进展[J].海南医学,2022,33(20):2716-2720. CAI W K,CAI S L,HAO Z J.Progress on mainstream constant temperature amplification technologies and its optimized strategy[J].Hainan Medical Journal,2022,33(20):2716-2720.(in Chinese)
[38] 史贇学,王鑫,王峻,等.重组酶恒温扩增技术在动植物源成分分析中的应用与研究进展[J].中国食品卫生杂志,2024,36(2):231-238. SHI Y X,WANG X,WANG J,et al.Application and research progress of recombinase isothermal amplification technology in the analysis of animal and plant components[J].Chinese Journal of Food Hygiene,2024,36(2):231-238.(in Chinese)
[39] MAYBORODA O,KATAKIS I,O’SULLIVAN C K.Multiplexed isothermal nucleic acid amplification[J].Analytical Biochemistry,2018,545:20-30.
[40] YAN S,LI C,LAN H,et al.Comparison of four isothermal amplification techniques:LAMP,SEA,CPA,and RPA for the identification of chicken adulteration[J].Food Control,2024,159:110302.
[41] 柴书军,郭军庆,刘运超,等.猪细小病毒7型LAMP-LFD快速检测方法的建立及应用[J].畜牧与兽医,2022,54(9):71-77. CHAI S J,GUO J Q,LIU Y C,et al.Establishment and application of an LAMP-LFD rapid detection method for Porcine parvovirus type 7[J].Animal Husbandry & Veterinary Medicine,2022,54(9):71-77.(in Chinese)
[42] 唐艳荣,张小梅,郭建立,等.鸽新城疫病毒可视化LAMP检测方法的建立与应用[J].中国家禽,2024,46(3):120-124. TANG Y R,ZHANG X M,GUO J L,et al.Establishment and application of visualization LAMP for detection of Pigeon Newcastle disease virus[J].China Poultry,2024,46(3):120-124.(in Chinese)
[43] 朱海鹏,郑旭,王慧,等.猪胸膜肺炎放线杆菌可视化LAMP检测方法的建立与应用[J].中国兽医科学,2022,52(7):837-845. ZHU H P,ZHENG X,WANG H,et al.Establishment and application of visual LAMP method for Actinobacillus pleuropneumoniae detection[J].Chinese Veterinary Science,2022,52(7):837-845.(in Chinese)
[44] LAI F,CHANG K,CHANG C,et al.Development of a rapid sex identification method for newborn pigeons using recombinase polymerase amplification and a lateral-flow dipstick on farm[J].Animals,2022,12(21):2969.
[45] CAKMAK E,AKIN P C,BILGIN C C.Comparison of three different primer sets for sexing birds[J].Journal of Veterinary Diagnostic Investigation,2017,29(1):59-63.
[46] TOMITA N,MORI Y,KANDA H,et al.Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products[J].Nature Protocols,2008,3(5):877-882.
[47] NUPUR G,FARHAN JALEES A,SUDESHNA K.Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens[J].Current Research in Microbial Sciences,2022,3:100120.
[48] SOROKA M,WASOWICZ B,RYMASZEWSKA A.Loop-mediated isothermal amplification (LAMP):The better sibling of PCR?[J].Cells,2021,10(8):1931.
[49] LI J,MACDONALD J,VON STETTEN F.A comprehensive summary of a decade development of the recombinase polymerase amplification[J].Analyst,2019,144(1):31-67.
[50] YANG Q,ZHAO X,WANG D,et al.A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus[J].Scientific Reports,2022,12(1):681.
[51] XU G,HU L,ZHONG H,et al.Cross priming amplification:Mechanism and optimization for isothermal DNA amplification[J].Scientific Reports,2012,2(1):246.
[52] NOTOMI T,OKAYAMA H,MASUBUCHI H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Research,2000,28(12):e63.
[53] NAGAMINE K,HASE T,NOTOMI T.Accelerated reaction by loop-mediated isothermal amplification using loop primers[J].Molecular and Cellular Probes,2002,16(3):223-229.
[54] 张慧.幼鸽早期性别PCR、LAMP和双重LAMP检测方法的建立及应用[D].乌鲁木齐:新疆农业大学,2022. ZHANG H.Establishment and application of PCR,LAMP and double LAMP on sex detecion method for young pigeons[D].Urumqi:Xinjiang Agricultural University,2022.(in Chinese)
[55] ELLEGREN H.First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non-ratite birds[J].Proceedings of the Royal Society of London.Series B:Biological Sciences,1996,263(1377):1635-1641.
[56] CHANGTOR P,GUPTA Y M,YIMTRAGOOL N.Optimization and application of loop-mediated isothermal amplification technique for sex identification in red-whiskered bulbul (Pycnonotus jocosus)[J].Ecology and Evolution,2022,12(10):e9401.
[57] 彭树英,雍德祥,王永生.LAMP法鉴定家鸡性别的初步研究[J].淮北师范大学学报(自然科学版),2014,35(4):52-56. PENG S Y,YONG D X,WANG Y S.Preliminary investigation of sex identification of Gallus by loop-mediated isothermal amplification[J].Journal of Huaibei Normal University(Natural Sciences),2014,35(4):52-56.(in Chinese)
[58] KIM S W,CHOI J S,SHARMA N,et al.A novel approach for determination of chicken sexing at an early stage of development by using loop-mediated isothermal amplification method[J].Turkish Journal of Veterinary & Animal Sciences,2015,39(5):583-588.
[59] 许晓琳,袁向芬,孔玉方,等.猴痘病毒实时荧光LAMP检测方法的建立[J].黑龙江畜牧兽医,2024,2:72-78. XU X L,YUAN X F,KONG Y F,et al.Establishment of Real-time fluorescent LAMP method for detection of Monkeypox virus[J].Heilongjiang Animal Science and Veterinary Medicine,2024,2:72-78.(in Chinese)
[60] CHAN K W,LIU P C,YANG W C,et al.A novel loop-mediated isothermal amplification approach for sex identification of Columbidae birds[J].Theriogenology,2012,78(6):1329-1338.
[61] BECHERER L,BORST N,BAKHEIT M,et al.Loop-mediated isothermal amplification (LAMP) -review and classification of methods for sequence-specific detection[J].Analytical Methods, 2020,12(6):717-746.
[62] 李巍,杨卓,陈明非,等.LAMP法鸟类性别鉴定的研究与应用[J].现代畜牧兽医,2017,3:12-18. LI W,YANG Z,CHEN M F,et al.Research and application of LAMP in bird sex identification[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2017,3:12-18.(in Chinese)
[63] CENTENO-CUADROS A,TELLA J L,DELIBES M,et al.Validation of loop-mediated isothermal amplification for fast and portable sex determination across the phylogeny of birds[J].Molecular Ecology Resources,2018,18(2):251-263.
[64] KOCH H R,BLOHM-SIEVERS E,LIEDVOGEL M.Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP)[J].Ecology and Evolution,2019,9(10):5849-5858.
[65] HAMER M,WATANABE O,SARAULLO V,et al.Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters[J].Veterinary Research Communications,2024,48(1):103-111.
[66] 田万年,李齐,袁世超.羊泰勒虫可视化LAMP检测方法的建立[J].贵州畜牧兽医,2023,47(5):53-56. TIAN W N,LI Q,YUAN S C.Establishment of a visualized LAMP detection method for Theileria[J].Guizhou Journal of Animal Husbandry & Veterinary Medicine,2023,47(5):53-56.(in Chinese)
[67] ELNOMROSY S M,HAGAG N M,ABDALLAH M I,et al.Application of loop-mediated isothermal amplification (LAMP) in sex identification of parrots bred in Egypt[J].Biology,2022,11(4):565.
[68] KANEKO H,KAWANA T,FUKUSHIMA E,et al.Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances[J].Journal of Biochemical and Biophysical Methods,2007,70(3):499-501.
[69] PIEPENBURG O,WILLIAMS C H,STEMPLE D L,et al.DNA detection using recombination proteins[J].PLoS Biology,2006,4(7):e204.
[70] 曾繁文,陈谭子芃,萨家祺,等.用于鹈鹕性别鉴定的RAA-LFD引物、试剂盒及方法：CN202410173759.3[P].2024-04-19. ZENG F W,CHEN T Z P,SA J Q,et al.RAA-LFD primers,kits and methods for pelican sex determination：CN202410173759.3 [P].2024-04-19.(in Chinese)
[71] 曾繁文,陈谭子芃,陈绚姣,等.一种用于鉴定天鹅性别的RPA引物、试剂盒及应用：CN202311598042.5[P].2024-05-07. ZENG F W,CHEN T Z P,CHEN X J,et al.An RPA primers,kit and application for sex identification in swans：CN202311598042.5[P].2024-05-07.(in Chinese)
[72] 邹衡,郑贤杰,陈松林,等.一种基于酶扩增的鸽子性别及病原感染鉴定用引物及探针：CN202311839096.6[P].2024-03-29. ZOU H,ZHENG X J,CHEN S L,et al.Primers and probes for sex and pathogenic infection identification in pigeons based on enzyme amplification：CN202311839096.6[P].2024-03-29.(in Chinese)
[73] 王姝然,范厚勇,徐嘉楠,等.弗氏柠檬酸杆菌RPA-LFD快速检测方法的建立及应用[J].大连海洋大学学报,2024,39(2):241-249. WANG S R,FAN H Y,XU J N,et al.Establishment and application of a method for rapid detection of Citrobacter freundii by recombinase polymerase amplification (RPA) combined with lateral flow strips (LFD)[J].Journal of Dalian Ocean University,2024,39(2):241-249.(in Chinese)
[74] 袁雪梅,陈静,黄雷,等.快速检测十足目虹彩病毒1 RPA-LFD方法的建立及初步应用[J].中国预防兽医学报,2024,46(5):505-510. YUAN X M,CHEN J,HUANG L,et al.Rapid visual detection of DIV1 by combinase recombinase polymerase amplification with lateral flow dipstick[J].Chinese Journal of Preventive Veterinary Medicine,2024,46(5):505-510.(in Chinese)
[75] 黄梦琦,李婧姝,邱孺,等.交叉引物恒温扩增技术(CPA)研究进展[J].民营科技,2018，9:78-79. HUANG M Q,LI J S,QIU R,et al.Research progress on crossing priming isothermal amplification (CPA)[J].Non-State Running Science & Technology Enterprises,2018,9:78-79.(in Chinese)
[76] 王伟.基于交叉引物等温扩增结合胶体金免疫层析试纸卡快速鉴别鸽子雌雄的方法建立[D].新乡:河南科技学院,2023. WANG W.Novel approach to rapidly identify the gender of the pigeon by using cross-priming amplification with immunochromatographic strip[D].Xinxiang:Henan Institute of Science and Technology,2023.(in Chinese)
[77] WANG Y M,WANG S,GAO Y L,et al.An approach to rapidly identify the gender of the pigeon by using cross-priming amplification with immune-chromatographic strip[J].Talanta,2024,269:125452.

Research Progress on Isothermal Amplification Technique in Sex Identification of Birds

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HAN Fengye, LIU Ying, PAN Chenfan, ZHANG Qianyi, CHEN Xiaoyun, ZHU Zhen, YIN Chunsheng, WEN Yongjun, WANG Fengxue, DU Jige. Expression and Preparation of Monoclonal Antibodies of Clostridium perfringens Alpha-Toxin Mutant [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 442-450.
[2]	YANG Yunyun, ZHANG Qiandong, YUAN Yang, WANG Wei, TIAN Qin, CAI Haiqing, LIU Xin, WEN Guilan, YANG Ying, CHENG Zhentao, WEN Ming. Metabolomic Analysis of Ileum Co-infected with Duck Enteritis Virus and Clostridium perfringens Type A in Ducks [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4759-4767.
[3]	SUN Junli, SUN Ruyu, LIAO Haihong, LU Jianqin, YAN Jing, LU Shengsheng, ZHANG Bing. Study on Porcine ICSI Embryo Sex Identification Based on Raman Spectroscopy and SVM [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(4): 1430-1437.
[4]	FAN Lixia, YUAN Xuexia, LI Yuanyang, WU Yuanjuan, ZHAO Shancang, ZHANG Bingchun, WANG Wenbo. LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(7): 2325-2335.
[5]	XIE Jing, YU Jifeng, ZHOU Long, CAO Ye, LIN Yi, LI Xingyu, XIAO Lu, YE Yonggang, PAN Meng, KANG Runmin. Establishment and Application of Visual LAMP Detection Method for Infectious Laryngotracheitis Virus [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(2): 565-572.
[6]	XU Yunming, WEI Libin, ZHOU Yanyang, LI Yehui, SUN Zhiyuan, ZHONG Siyuan, CHEN Aining, GUO Lijuan, LIU Zengshan, REN Honglin. Establishment of Loop-mediated Isothermal Amplification (LAMP) Method for Klebsiella pneumoniae [J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(11): 3003-3010.
[7]	LIU Wenjun, HUANG Yunmao, YANG Youtian, XU Danning, CAO Nan, ZHOU Derong, TIAN Yunbo. Establishment of RT-LAMP Visual Detection Method for Goose Newcastle Disease Virus [J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(1): 22-31.
[8]	JIA Chun-yan, JI Xiao-yang, BAI Xue, DAI Hao-yang, WANG Jian-meng, ZHANG Wen-guang. Regulatory Mechanisms of Long Noncoding RNAs and Forecasting Methods in Livestock [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(7): 1895-1905.
[9]	CHEN Xi-wen, WANG Qian, YIN Miao, LI Lian, LUO Wen-tao, YANG Feng, GUO Ai-wei, ZHOU Jie-long, WANG Xiong-qing. Establishment of RT-LAMP for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(6): 1630-1636.
[10]	LUO Ya-kun, LIANG Lin, WANG Jing, LIU Cun, LIU Qi, LIU Chang, LIN Wen-cheng, CUI Shang-jin. Establishment and Application of Loop-mediated Isothermal Amplification for Rapid Detection of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(2): 344-349.
[11]	LV Ji-zhou, FAN Yan-ru, FENG Chun-yan, YUAN Xiang-fen, WU Shao-qiang. Establishment of Real-time Recombinase Polymerase Amplification for Detection of Porcine Epidemic Diarrhoea Virus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(12): 3434-3439.
[12]	LIU Li-bing, WANG Jian-chang, JING Mei, WANG Jin-feng, YUAN Wan-zhe. Development of the Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Parvovirus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(11): 3320-3326.
[13]	WU Zhi, XIA Wen-long, CAI Shu-dong, GUO Chang-ming, YUAN Wei-feng, WANG Yong-juan. Establishment and Application of Visual RT-LAMP for Rapid Detection of Duck Tembusu Virus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(11): 3334-3339.
[14]	LI Jian-you, LI Jia-wei, WANG Chao, GUO Li, HE Hong-bin. Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus [J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(10): 2837-2844.
[15]	YANG Min, WANG Hui-dang, YU Lu, WU Chang-xin, Zhang Hui, CAO Xu-dong, CHEN Chuang-fu. Study on Optimization of the Conditions of Detecting Mycobacterium tuberculosis in Milk by Technique of Loop-mediated Isothermal Amplification [J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(7): 1688-1693.