饲料中添加不同剂量缩合单宁对花鲈生长性能、肝脏和肠道形态及肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2025.08.005

Abstract

Abstract: 【Objective】 This study was conducted to investigate the effects of different dose of condensed tannins (CT) on growth performance,liver and intestinal morphology and the intestinal flora of Lateolabrax maculatus. 【Method】 Four diets were prepared by adding 0 (CT0),1 (CT1),3 (CT3) and 5 (CT5) g/kg of CT in the basal diet,respectively.Fish (initial weight of 4.38 g±0.01 g) were randomly divided into four groups,with three replicates in each group and a total of 35 fish in each replicate.The feeding trial was lasted for 45 days.At the terminal of the feeding trial,the total quantity,total weight and total food intake of each tank were counted to calculate the growth performance.Three fish were randomly selected from each tank,and the liver and intestine were dissected to prepare paraffin sections and analyze the tissue morphology.Another three fish were randomly selected from each tank to isolate the intestinal contents and determine the composition and diversity of intestinal bacteria. 【Result】 Compared with CT0 group,fish in CT1 group had similar growth performance (P＞0.05),the final body weight,weight gain,specific growth rate,and feed intake of fish in CT3 and CT5 groups were lower (P＜0.05),whereas the feed coefficient was higher (P＜0.05).In CT1,CT3 and CT5 groups,the liver showed varying degrees of vacuolization,fibrosis,and inflammatory infiltration,with the most severe tissue damage observed in CT5 group.Dietary inclusion of CT caused intestinal damage,with fish in CT5 group showing severe atrophy of the intestinal villi and obvious structural damage.At the phylum level,the intestinal microbiota of fish was mainly composed of Proteobacteria and Firmicutes.The relative abundance of Proteobacteria presented a trend of first decreasing and then increasing but was not significant (P＞0.05),while the relative abundance of Bacteroidetes,Chlorobi and Chloroflexi presented a trend of first increasing and then decreasing,and they were higher in CT3 group (P＜0.05).At the genus level,the intestinal microbiota of fish was mainly composed of Methylobacterium and Ralstonia.Compared with CT0 group,the relative abundance of Methylobacterium in CT1,CT3,and CT5 groups were significantly decreased (P＜0.05).The relative abundances of Bacteroides,Faecalibacterium,Roseburia and Sutterella in CT3 group were significantly increased (P＜0.05).Compared with CT0 group,the Shannon index in CT3 group was significantly increased (P＜0.05). 【Conclusion】 Dietary inclusion of 1 g/kg of CT did not alter the growth performance,whereas 3 and 5 g/kg of CT significantly reduced the feed intake and growth performance,damaged the morphology of liver and intestine,and interfered with the intestinal microflora composition of Lateolabrax maculatus.

Key words: condensed tannins; Lateolabrax maculatus; tissue morphology; intestinal microflora

CLC Number:

S816.7

DONG Ruiqi, QIU Jianqiang, SUN Wenhao, HUANG Wen, CAO Junming, ZHAO Hongxia, CHEN Bing, WANG Guoxia, ZHU Xifeng, PENG Kai. Effects of Different Dose of Condensed Tannins on Growth Performance,Liver and Intestinal Morphology and Intestinal Flora of Lateolabrax maculatus[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(8): 3551-3562.

References

[1] 王思琪,孔祥祎,杨梦然,等.不同来源单宁在畜禽养殖中的应用[J].中国畜牧兽医,2023,50(8):3102-3112. WANG S,KONG X,YANG M,et al.Application of different sources of tannin in livestock farming[J].China Animal Husbandry & Veterinary Medicine,2023,50(8):3102-3112.(in Chinese)
[2] 彭凯,王玉玺,王国霞,等.缩合单宁的生物功能及其在动物生产中的应用[J].动物营养学报,2020,32(8):3451-3460. PENG K,WANG Y,WANG G,et al.Biological function of condensed tannins and their application in animal production[J].Chinese Journal of Animal Nutrition,2020,32(8):3451-3460.(in Chinese)
[3] CHUNG K T,WEI C I,JOHNSON M G.Are tannins a doubleedged sword in biology and health[J].Trends in Food Science and Technology,1998,9(4):168-175.
[4] 彭凯,陈冰,莫文艳,等.饲料中添加缩合单宁对凡纳滨对虾生长性能、体成分、血清生化及抗氧化指标的影响[J].动物营养学报,2022,34(2):1165-1174. PENG K,CHEN B,MO W Y,et al.Effects of dietary condensed tannins on growth performance,body composition,serum biochemical and antioxidant indexes of Litopenaeus vannamei[J].Chinese Journal of Animal Nutrition,2022,34(2):1165-1174.(in Chinese)
[5] 彭凯,陈冰,赵红霞,等.缩合单宁对海鲈生长性能、体成分、营养物质表观消化率及肠道组织结构的影响[J].中国畜牧兽医,2022,49(8):2953-2960. PENG K,CHEN B,ZHAO H X,et al.Effects of condensed tannins on growth performance,body composition,apparent digestibility of nutrients,and intestinal morphology of Lateolabrax maculatus[J].China Animal Husbandry & Veterinary Medicine,2022,49(8):2953-2960.(in Chinese)
[6] PENG K,LV X,ZHAO H,et al.Antioxidant and intestinal recovery function of condensed tannins in Lateolabrax maculatus responded to in vivo and in vitro oxidative stress[J].Aquaculture,2022,547:737399.
[7] PENG K,ZHOU Y,WANG Y,et al.Inclusion of condensed tannins in Lateolabrax japonicus diets:Effects on growth,nutrient digestibility,antioxidant and immune capacity and copper sulphate stress resistance[J].Aquaculture Reports,2020,18:100525.
[8] PENG K,WANG G,WANG Y,et al.Condensed tannins enhanced antioxidant capacity and hypoxic stress survivability but not growth performance and fatty acid profile of juvenile Japanese seabass (Lateolabrax japonicus)[J].Animal Feed Science and Technology,2020,269:114671.
[9] PENG K,ZHAO H,WANG G,et al.Effect of condensed tannins on growth performance,intestinal immune capacity and bacterial microbiomes of Lateolabrax japonicus[J].Aquaculture Research,2021,52(11):5321-5331.
[10] OMNES M H,LE G J,LE D H,et al.Effects of dietary tannin on growth,feed utilization and digestibility,and carcass composition in juvenile European seabass (Dicentrarchus labrax L.)[J].Aquaculture Reports,2017,6:21-27.
[11] 黎梅.缩合单宁对生长中期草鱼生产性能、肠道结构和免疫功能的影响及其机制[D].成都:四川农业大学,2019. LI M.Effects of condensed tannins on growth performance,intestinal structure and immune function as well as the mechanisms in on-growing grass carp (Ctenopharyngodon idella)[D].Chengdu:Sichuan Agricultural University,2019.(in Chinese)
[12] MANZOOR F,NISA M N,HUSSAIN H A,et al.Effect of chestnut hydrolysable tannin on weight management and ovarian histopathology of healthy female rats[J].The Journal of Animal and Plant Sciences,2021,31(3):831-840.
[13] CHEN B,QIU J,WANG Y,et al.Condensed tannins increased intestinal permeability of Chinese seabass (Lateolabrax maculatus) based on microbiome-metabolomics analysis[J].Aquaculture,2022,560:738615.
[14] LI M,FENG L,JIANG W D,et al.Condensed tannins decreased the growth performance and impaired intestinal immune function in on-growing grass carp (Ctenopharyngodon idella)[J].British Journal of Nutrition,2020,123(7):737-755.
[15] 康鹏.菜粕替代鱼粉及缩合单宁对大口黑鲈生长生理机能的效应研究[D].上海:上海海洋大学,2023. KANG P.Research on the effects of replacing fishmeal with rapeseed meal and dietary condensed tannins on growth performance and physiological function of largemouth bass (Micropterus salmoides)[D].Shanghai:Shanghai Ocean University,2023.(in Chinese)
[16] ZHENG L Z,WANG Z Z,ZHANG B,et al.Rhodiola rosea L.improved intestinal digestive enzyme activities,inflammatory response,barrier and microbiota dysbiosis in Lateolabrax maculatus juveniles fed with high-carbohydrate diets[J].Fish and Shellfish Immunology,2024,146:109362.
[17] MA J R,KONG L M,ZHOU S S,et al.Effect of supplementation of chlorogenic acid to high-fat diet on growth,lipid metabolism,intestinal and hepatic histology,and gut microbiota of spotted sea bass (Lateolabrax maculatus)[J].Metabolites,2023,13:1067.
[18] CHEN B H,LIU G H,CHEN Q R,et al.Discovery of a novel marine Bacteroidetes with a rich repertoire of carbohydrate-active enzymes[J].Computational and Structural Biotechnology Journal,2024,23:406-416.
[19] KARLSSON F H,USSERY D W,NIELSEN J,et al.A closer look at bacteroides:Phylogenetic relationship and genomic implications of a life in the human gut[J].Microbial Ecology,2011,61(3):473-485.
[20] 鲜文东,张潇橦,李文均.绿弯菌的研究现状及展望[J].微生物学报,2020,60(9):1801-1820. XIAN W D,ZHANG X Z,LI W J.Research status and prospect on bacterial phylum Chloroflexi[J].Acta Microbiologica Sinica,2020,60(9):1801-1820.(in Chinese)
[21] BOWSER S M,MOORE W T,MCMILLAN R P,et al.High-molecular-weight cocoa procyanidins possess enhanced insulin-enhancing and insulin mimetic activities in human primary skeletal muscle cells compared to smaller procyanidins[J].The Journal of Nutritional Biochemistry,2017,39:48-58.
[22] PINENT M,BLADE M C,SALVADO M J,et al.Metabolic fate of glucose on 3T3-L1 adipocytes treated with grape seed-derived procyanidin extract (GSPE).Comparison with the effects of insulin[J].Journal of Agricultural and Food Chemistry,2005,53(15):5932-5935.
[23] 孙文豪,邱建强,田仁红,等.高糖饲料及添加缩合单宁对花鲈生长性能、体成分、血清代谢物、肠道短链脂肪酸含量及激素基因表达的影响[J].动物营养学报,2024,36(8):5254-5268. SUN W H,QIU J Q,TIAN R H，et al.Effects of high sugar diet and condensed tannin supplementation on growth performance,body composition,serum metabolites,intestinal short-chain fatty acid contents and hormone gene expression of sea bass (Lateolabrax maculatus)[J].Chinese Journal of Animal Nutrition,2024,36(8):5254-5268.(in Chinese)
[24] KOVALEVA J,DEGENER J E,MEI H C.Methylobacterium and its role in health care-associated infection[J].Journal of Clinical Microbiology,2014,52(5):1317-1321.
[25] MARIN L,MIGUELEZ E M,VILLAR C J,et al.Bioavailability of dietary polyphenols and gut microbiota metabolism:Antimicrobial properties[J].BioMed Research International,2015,2015(1):905215.
[26] TSAI S M,VAZOLLER R F,PAZINATO J M,et al.Molecular characterization of the archaeal community in an amazonian wetland soil and culture-dependent isolation of methanogenic archaea[J].Diversity,2010,2(6):1026-1047.
[27] PELISSIER R,COUTERON P.An operational,additive framework for species diversity partitioning and beta diversity analysis[J].Journal of Ecology,2007,95(2):294-300.

Effects of Different Dose of Condensed Tannins on Growth Performance,Liver and Intestinal Morphology and Intestinal Flora of Lateolabrax maculatus

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