24味中草药与抗生素联合对2种病原菌的抑菌作用研究

doi:10.16431/j.cnki.1671-7236.2025.07.039

Abstract

Abstract: 【Objective】 The experiment aimed to study the bacteriostatic effect of commonly used antibiotics and 24 Chinese herbal medicines on Aeromonas hydrophila and Bacillus cereus,as well as the bacteriostatic effect of the combination of drugs,so as to select effective antibacterial formulas for production practice and treat bacterial infections. 【Method】 Several antibiotics with strong inhibitory effect on Aeromonas hydrophila and Bacillus cereus were screened by drug sensitive disk method,and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these antibiotics and 24 Chinese herbal medicines against the above two pathogens were determined.According to the MIC and MBC of Chinese herbal medicine,the effective Chinese herbal medicine was selected to be combined with antibiotics.The fractional inhibitory concentrations index (FICI) of the combined drugs against Aeromonas hydrophila and Bacillus cereus was determined by the broth chessboard dilution method.The combined effect of drugs was judged,and the effective combination of Chinese and Western medicine was selected.One group of Chinese and Western medicine was made into a combined prescription for in vivo test. 【Result】 The antibacterial effect of antibiotics and Chinese herbal medicine on Aeromonas hydrophila showed that the antibiotic enrofloxacin had the best effect (MIC was 0.39 mg/mL),and the Chinese herbal medicine Scutellaria baicalensis Georgi had the best effect (MIC was 7.81 mg/mL).The results of combined medication showed that the FICI of Scutellaria baicalensis Georgi and enrofloxacin was 0.127,showing a synergistic effect,and the FICI of Pericarpium granati and enrofloxacin was 0.562,showing an additive effect.The antibacterial effect of antibiotics and Chinese herbal medicine on Bacillus cereus showed that the antibiotic ceftazidime had the best effect (MIC was 0.78 mg/mL),and the Chinese herbal medicine Coptis chinensis Franch.and Scutellaria baicalensis Georgi had the best effect (MIC both were 7.81 mg/mL).The results of combined medication showed that the FICI of Coptis chinensis Franch.and ceftazidime was 0.5,showing a synergistic effect.The in vivo test results of the combined medication showed that Pericarpium granati and enrofloxacin combined medication group had complete liver structure,closely arranged hepatocytes,at the same time,the intestinal tissues were clearly defined,and the intestinal mucosa was intact,and had a good protective effect on the intestinal mucosa,effectively alleviating the damage caused by Aeromonas hydrophila to the liver and intestine of Micropterus salmoides. 【Conclusion】 In vitro test,Scutellaria baicalensis Georgi combined with enrofloxacin and Pericarpium granati combined with enrofloxacin had inhibitory effect on Aeromonas hydrophila.Coptis chinensis Franch.combined with ceftazidime had strong antibacterial effect on Bacillus cereus.The combination of Pericarpium granati and enrofloxacin had a good therapeutic effect on Aeromonas hydrophila infected Micropterus salmoides.

Key words: Chinese herbal medicine; antibiotic; Aeromonas hydrophila; Bacillus cereus; fractional inhibitory concentration index (FICI)

CLC Number:

S853.7

HUANG Chunxing, LIANG Yanda, LAI Mingjian, WEI Mulian, YANG Ying. Study on the Antibacterial Effect of 24 Chinese Herbs Medicines Combined with Antibiotics on Two Pathogenic Bacteria[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3409-3419.

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Study on the Antibacterial Effect of 24 Chinese Herbs Medicines Combined with Antibiotics on Two Pathogenic Bacteria

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