牦牛源产细菌素屎肠球菌SWUN5732基因组测序及抑菌相关基因分析

doi:10.16431/j.cnki.1671-7236.2023.11.023

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the essential functional genes of Enterococcus faecium SWUN5732, a strain isolated from yak, through an analysis of its genome sequence detection outcomes.【Method】 Through Illumina PE150 sequencing system, the genome of Enterococcus faecium SWUN5732 was sequenced, and the basic functional characteristics of the genome were annotated in GO, KEGG, COG and NR databases.Combined with the annotation of NR database, the potential antibacterial, antioxidant and antibacterial peptide related genes in the genome were excavated.【Result】 The genome size of Enterococcus faecium SWUN5732 was 2 824 168 bp, and the effective average GC-content was about 38.09%.The number of detectable coding genes was approximately 2 919, and the total length of all coding genes was approximately 2 387 787 bp, with an average length of approximately 818 bp.In the GO database, a total of 8 851 genes in the SWUN5732 genome were annotated, with 45 entries in three major categories:Molecular function, cellular component and biological process.In the KEGG database, there were a total of 1 534 genes annotated in the SWUN5732 genome, including 38 pathways for six major functions:Cellular processes, environmental information processing, genetic information processing, human diseases, metabolism, and biological systems.In the COG database, a total of 2 118 genes had been annotated in the SWUN5732 genome, which also included four functional sequences with ABC type amino acid transport systems (osmotic enzyme components) and sequences of ABC transport proteins with repetitive ATPase domains.According to the functional annotation of Enterococcus faecium SWUN5732 in NR database, a total of 2 844 genes were annotated, and it was found that the genome of SWUN5732 contained antibacterial substances, antioxidant substances and antibacterial peptides related genes.The phylogenetic tree analysis results indicated that the strain had the closest evolutionary relationship to ATCC 19434.【Conclusion】 In this study, the genome of bacteriocin-producing Enterococcus faecium SWUN5732 from yaks was sequenced and potential antimicrobial substances were excavated, which confirmed that the strain had acid and bile salt tolerance environmental adaptability and explored the genes related to antioxidant, immune regulation, bacteriocin-production and antimicrobial substances, providing a reliable basis for the strain as an antibiotic substitute or feed probiotic additive.

Key words: yak yogurt; Enterococcus faecium; genome sequencing; gene function annotation; bacteriocin

CLC Number:

S852.6

PU Sicheng, ZHENG Huibin, RAN Xuan, YANG Falong, TANG Cheng, LIU Yuanyuan, CHEN Dechun. Genome Sequencing and Antimicrobial Related Gene Analysis of Bacteriocin-producing Enterococcus faecium SWUN5732 from Yak[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4545-4556.

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Genome Sequencing and Antimicrobial Related Gene Analysis of Bacteriocin-producing Enterococcus faecium SWUN5732 from Yak

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