散养黄羽肉鸡CAPN1、H-FABP基因表达的发育性变化及其肌内脂肪含量的研究

doi:10.16431/j.cnki.1671-7236.2019.05.019

Abstract

Abstract:

In order to investigate the relationship between CAPN1,H-FABP genes and the growth and intramuscular fat contentcontent (IMF) of Yellow-feathered broilers under the free-range feeding system,130 3-week-old health Yellow-feathered broiler hens with similar weight were selected as test material and randomly divided into two groups with cage rearing (control group) and free-range group (test group).A total of 360 samples of chest muscles,leg muscles and abdominal fat of Yellow-feathered broiler hens were collected,the CAPN1 and H-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content was determined by the method of Soxhlet extraction.The results showed that the CAPN1 and H-FABP mRNAs of Yellow-feathered broilers were expressed in different degrees in the chest muscles,leg muscles and abdominal fat.With the increase of age,the overall expression trend of CAPN1 in free-range group was increase-decrease-increase-decrease,and the expression level of CAPN1 mRNA in chest and abdominal fat were significantly higher in cage rearing than that in free-range during 3 to 6 w (P<0.05).The overall expression trend of H-FABP decreased with the increasing age,and the expression trends in the chest and leg muscles were the same and they were significantly higher in free-range than the cage rearing (P<0.05).The expression of H-FABP in abdominal fat was higher in cage rearing group (P>0.05).Compared to free-range chickens,cage rearing chickens grew faster,and the rate of abdominal fat and IMF content were higher also.The test results could provide some reference for the CAPN1 and H-FABP genes molecular breeding of Yellow-feathered chickens.

Key words: Yellow-feathered broiler; CAPN1 gene; H-FABP gene; intramuscular fat

CLC Number:

S813

ZHU Mengting, XIE Yifan, WANG Xiaolu, ZHAO Zongsheng. Developmental Changes of CAPN1 and H-FABP Genes Expression and Their Intramuscular Fat Content in Yellow Feather Broilers Under Free-range[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(5): 1413-1420.

References

[1] 邹永新,肖智远.广东农村散养鸡健康养殖现状与环境保护[J].养殖与饲料,2017,8:105-108. ZHOU Y X,XIAO Z Y.Current status and environmental protection of healthy breeding of free-range chickens in rural Guangdong[J].Farming and Feed,2017,8:105-108.(in Chinese)
[2] 李如兰,戴四发.肉鸡皮肤黄色素沉积的研究进展[J].家禽科学,2008,11:40-43. LI R L,DAI S F.Advances in research on yellow pigmentation of broiler skin[J]. Poultry Science,2008,11:40-43.(in Chinese)
[3] LEE K H,LEESON S.Performance of broilers fed limited quantities of feed or nutrients during seven to fourteen days of age[J]. Journal of Poultry Science,2001,80(4):446-454.
[4] 牛竹叶,刘福柱,刘志芳,等.早期限饲对肉仔鸡生产性能与肥度的影响[J].西北农林科技大学学报,2001,29(4):21-23. NIU Z Y,LIU F Z,LIU Z F,et al.Effect of early feed restriction on performance and fertility of broilers[J].Journal of Northwest A & F University,2001,29(4):21-23.(in Chinese)
[5] HOEQUETTE J F,HOCQUETTE J F,GONDRET F,et al.Intramuseular fat content in meat-producing animals:Development genetic and nutritional control and identification of Putative markers[J].Animal,2010,4(2):303-319.
[6] 杨烨.优质鸡肌内脂肪代谢调控及其与肉质性状关系的研究[D].北京:中国农业科学院,2005. YANG Y.Studies on the metabolism of intramuscular fat in quality chicken and its relationships with meat quality traits[D].Beijing:Chinese Academy of Agricultural Sciences,2005.(in Chinese)
[7] DEVOL D L,MCKEITH P,BECHTEL P J,et al.Variation incomposition and palatability traits and relationships between muscle characteristics and palatability in a random sample of pork carcasses[J].Animal Science,1988,66:385-395.
[8] 陈继兰.鸡肉肌苷酸和肌内脂肪含量遗传规律及相关候选基因的研究[D].北京:中国农业大学,2004. CHEN J L.Studies on inheritance and candidate genes of inosine-5'- monophosphate and intramuscular fat contents in chicken meat[D].Beijing:China Agricultural University,2004.(in Chinese)
[9] 李建军.优质肉鸡风味特性研究[D].北京:中国农业科学院,2003. LI J J.Study on flavor characteristics of high quality broiler[D]. Beijing:Chinese Academy of Agricultural Sciences,2003.(in Chinese)
[10] GLITSEH K.Consumer percreptions of fresh meat quality:Cross-national comparison[J].British Food Journal,2000,102(3):177-194.
[11] 田璐,岳文斌,李姣,等.中国西门塔尔牛CAPN1基因SNPs及其与经济性状关联分析[J].畜牧兽医学报,2011,42(6):785-789. TIAN L,YUE W B,LI J,et al.SNPs of CAPN1 gene and their association with some economic traits of Chinese Simmental cattle[J].Journal of animal husbandry and veterinary,2011,42(6):785-789.(in Chinese)
[12] 李文娟,李宏宾,文杰,等.鸡H-FABP和A-FABP基因表达与肌内脂肪含量相关研究[J].畜牧兽医学报,2006,37(5):417-423. LI W J,LI H B,WEN J,et al.Association of the H-FABP and A-FABP genes expression with intramuscular fat content in chickens[J].Chinese Journal of Animal Husbandry and Veterinary Sciences,2006,37(5):417-423.(in Chinese)
[13] BOEKHOLT H,VAN DER GRINTEN P,SCHREURS V,et al.Effect of dietary energy restriction on retention of protein,fat and energy in broiler chickens[J].British Poultry Science,1994,35:603.
[14] KEMP C M,SENSKY P L,BARDSLEY R Q,et al.Tenderness——An enzymatic view[J].Meat Science,2010,84:248-256.
[15] GEESINK G H,KOOHMARAIE M.Ionic strength-induced inactivation of mu-calpain in postmortem muscle[J].Journal of Animal Science,2000,78:2336.
[16] KOOHMARAIE M,KENT M P,SHACKELFORD S D,et al.Meat tenderness and muscle growth:Is there any relationship?[J].Meat Science,2002,62:345-352.
[17] LI H F,WU G,ZHANG J,YANG N.Identification of the heart-type fatty acid-binding protein as a major gene for chicken fatty acid metabolism by Bayesian network analysis[J].Poultry Science,2010,89:1825.
[18] 肖雅婷,邹爱珍.3种不同类型鸡屠宰性能的测定与比较[J].畜牧与饲料科学,2015,36(4):34-36. XIAO Y T,ZOU A Z.Determination and comparison of slaughter performance of 3 different types of chickens[J].Animal Husbandry and Feed Science,2015,36(4):34-36.(in Chinese)
[19] 王玉玲,丁淑芬,宋岩,等.索氏抽提法测定粗脂肪含量方法的探讨[J].粮食加工,2014,39(5):76-77. WANG Y L,DING S F,SONG Y,et al.Discussion of Soxhlet fat content determination[J].Grain Processing,2014,39(5):76-77.(in Chinese)
[20] 邵勇钢,臧长江,曾伟丹,等.拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析[J].中国畜牧兽医,2017,44(1):141-147. SHAO Y G,ZANG C J,ZENG W D,et al.Polymorphism of A-FABP gene and its association analysis with production performance in male Baicheng fatty chicken[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):141-147.(in Chinese)
[21] 周志楠,毛海彭,丁秋芸,等.巨型玫瑰冠鸡H-FABP、A-FABP基因表达的发育性变化及其肌内脂肪含量研究[J].中国畜牧兽医,2018,45(5):1289-1296. ZHOU Z N,MAO H P,DING Q Y,et al.The developmental changes of H-FABP and A-FABP genes expression and intramuscular fat content of Rose cockscomb chicken[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1289-1296.(in Chinese)
[22] SMITH T P,CASAS E,REXROAD C E,et al.Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness[J].Journal of Animal Science,2000,78:2589-2594.
[23] DEEB N,L AMONT S J.Genetic architecture of growth and body composition in unique chicken population[J].Hered,2002,93:107-118.
[24] PINTO L F B,FERRAZ T B S,MEIRELLES F V,et al.Association of SNPs on CAPN1 and CAST genes with tenderness in Nellore cattle[J].Genetics Molecular Research,2010,9(3):1431-1442.
[25] PAGE B T,CASAS E,QUAASR L,et al.Association of markers in the bovine CAPN1 gene with meat tenderness in large crossbred populations that sample influential industry sires[J].Journal of Animal Science,2004,82(12):3474-3481.
[26] 张增荣.鸡CAPN1、CAPN2、CAPN3和CAST基因的克隆、表达及其在肉质中的遗传效应分析[D].雅安:四川农业大学,2009. ZHANG Z R.Molecule clone,expression and the genetic effects in meat quality of chicken calpain 1,2,3 and calpastatin genes[D].Ya'an:Sichuan Agricultural University,2009.(in Chinese)
[27] 肖蔹,张增荣,黄毅,等.山地乌骨鸡不同组织中CAPN1基因表达的发育性变化[J].四川畜牧兽医,2010,37(5):34-37. XIAO L,ZHANG Z R,HUANG Y,et al.Developmental changes of CAPN1 gene expression in tissues of mountain Black-bone chicken[J].Sichuan Livestock Veterinary,2010,37(5):34-37.(in Chinese)
[28] BONEN A,LUIKEN J F,LUI S,et al.Palmitate transport and fatty acid transporters in red and white muscular[J].Journal of Endocrinology,1998,275:E471-E478.
[29] BINAS B,HAN X X,EROL E,et al.A null mutation in H-FABP only partially inhibits skeletal muscule fatty acid metabolism[J].Journal of Endocrinology,2003,285:E481-E489.
[30] 王启贵,李宁,邓学梅,等.鸡脂肪酸结合蛋白基因的克隆和测序分析[J].遗传学报,2002,2:115-118. WANG Q G,LI N,DENG X M,et al.Cloning and sequencing analysis of chicken fatty acid binding protein gene[J].Journal of Genetics and Genomics,2002,2:115-118.(in Chinese)
[31] GERBENS F,DE KONING D J,HARDERS F L,et al.The effect of adipocyte and heart fatty acid binding protein gene on intramusclar fat and backfat content in Meishan crossbred pigs[J]. Journal of Animal Science,2000,78:552-559.
[32] CHEN C Y,HU H C,LEE B C,et al.Exercise training improves cardiac function in infarctedrabbits:Involvement of autophagic function and fatty acid utilization[J].European Journal of Heart Failure,2010,12(4):323-330.
[33] 谢莉.CAPN1、CAST、H-FABP基因的表达及其在不同饲养方式下对鸡肉品质性状的影响[D].雅安:四川农业大学,2012. XIE L.Study the regularity of CAPN1,CAST,H-FABP genes expression and their effects on poultry meat quality trait in different rearing systems[D].Ya'an:Sichuan Agricultural University,2012.(in Chinese)
[34] 屠云洁,苏一军,王克华,等.利用实时荧光定量RT-PCR检测鸡A-FABP和H-FABP基因的差异表达[J].中国畜牧杂志,2010,46(7):1-4. TU Y J,SUN Y J,WANG K H,et al.Detection of different expression of chicken A-FABP and H-FABP genes by Real-time fluorescene quantitative RT-PCR[J].Chinese Journal of Animal Science,2010,46(7):1-4.(in Chinese)
[35] 屠云洁.实时荧光定量RT-PCR检测不同鸡种肉品质相关功能基因A-FABP和H-FABP的表达[A].中国畜牧业协会禽业分会首届中国黄羽肉鸡行业发展大会会刊[C].2008. TU Y J.Relative expression of A-FABP and H-FABP gens in chicken measured by Real-time PCR[A].The first China Yellow Feather Broiler Industry Development Conference,China Animal Husbandry Association Poultry Industry Branch[C].2008.(in Chinese)
[36] 呼红梅,王继英,郭建凤,等.莱芜猪和杜洛克猪肌肉H-FABP基因表达量与肌内脂肪和脂肪酸含量关联分析[J].华北农学报,2010,25(4):64-68. HU H M,WANG J Y,GUO J F,et al.Correlation analysis between muscle H-FABP gene expression and intramuscular fat and fatty acid content in Laiwu pig and Duroc pig[J].North China Agricultural Journal,2010,25(4):64-68.(in Chinese)
[37] 庞卫军,孙世铎,李影,等.西部地区主要猪种和野猪H-FABP基因分子标记与IMF含量关系[J].遗传,2005,3:351-356. PANG W J,SUN S Z,LI L,et al.Relationship between molecular markers of H-FABP gene and IMF content in main pig breeds and wild pigs in Western China[J]. Genetic,2005,3:351-356.(in Chinese)
[38] 李平,燕志宏,陈伟,等.宗地花猪H-FABP基因mRNA的表达与肉质性状的相关研究[J].中国畜牧兽医,2015,42(3):644-649. LI P,YAN Z H,CHEN W,et al.Association analysis on H-FABP gene expression level and meat quality traits of Zongdi spotted pigs[J].China Animal Husbandry & Veterinariy Medicine,2015,42(3):644-649.(in Chinese)
[39] 杨烨,文杰,陈继兰,等.饲养方式对河田鸡脂肪代谢的影响[J].畜牧兽医学报,2008,8:1050-1055. YANG Y,WEN J,CHEN J L,et al.Effect of feeding methods on fat metabolism of Hetian chicken[J].Chinese Journal of Animal Husbandry and Veterinary Sciences,2008,8:1050-1055.(in Chinese)
[40] MILOSEVIC N,PERIC L,SUPIC B.Raising chickens on a free range system Ⅰ.Evaluation of carcass quality[J].Biotechnology in Animal Husbandry,2003,19(5-6):335-343.
[41] CHARTRIN P,METEAU K,JUIN H,et al.Effects of intramuscular fat levels on sensory characteristics of duck breast meat[J].Poultry Science,2006,85:914-922.
[42] RUIZ J A,GUERRERO L,ARNAU J,et al.Descriptive sensory analysis of meat from broilers fed dietscontaining vitamin E or beta-carotene as antioxidants and different supplemental fats[J].Poultry Science,2001,80:976-982
[43] 武书庚,齐广海.肉品风味的形成及其影响因素[J].中国畜牧杂志,2001,3:53-55. WU S G,QI G H.The formation of meat flavor and its influencing factors[J].Chinese Journal of Animal Science,2001,3:53-55.(in Chinese)

Developmental Changes of CAPN1 and H-FABP Genes Expression and Their Intramuscular Fat Content in Yellow Feather Broilers Under Free-range

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